vegfa Search Results


99
Thermo Fisher gene exp vegfa hs00900055 m1
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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93
Boster Bio human vegf vegfa elisa kit
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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95
Proteintech anti vegfa
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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96
Proteintech ip vegfa proteintech 19003-1-ap
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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90
OriGene vegf
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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90
OriGene shorthairpin rna shrna oligonucleotides targeting vegfa
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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90
Boster Bio bsa
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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93
St Johns Laboratory anti vegfa
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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93
Proteintech vegfa
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
Vegfa, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
OriGene anti ddk
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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90
OriGene hp202779
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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90
ProSci Incorporated rabbit polyclonal antibody
( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and <t>VEGFA</t> ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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Image Search Results


( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and VEGFA ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Biology

Article Title: Comparative Effects of Dexamethasone and ASC Secretome in an Ex Vivo Osteoarthritis Co-Culture Model

doi: 10.3390/biology15060493

Figure Lengend Snippet: ( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and VEGFA ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Subsequently, the expression levels of different genes were quantified through reverse transcription quantitative polymerase chain reaction (RT-qPCR) employing TaqMan technology using the following probes: PTGS2 (hs00153133_m1), MMP3 (hs00968305_m1), MMP13 (hs00 233992_m1), SOX9 (hs00165814_m1), COL2A1 (hs01060345_m1), IL-1β (Hs01555410_m1), TIMP1 (Hs99999139_m1), ACAN (hs00153936_m1), COL10A1 (hs00166657_m1), RUNX2 (hs00231692_m1), VEGFA (hs00900055_m1), FN1 (Hs01549976_m1), CTGF (Hs00170014_m1), and TBP (Hs00427620_m1) (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Gene Expression, Expressing, Western Blot, Membrane