vector pgem t Promega Search Results


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    Promega pgem t promega vector
    Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: <t>pGEM-T/EcoRI/XhoI;</t> 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.
    Pgem T Promega Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t promega vector/product/Promega
    Average 99 stars, based on 3411 article reviews
    Price from $9.99 to $1999.99
    pgem t promega vector - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Promega pgem t easy promega vector
    Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: <t>pGEM-T/EcoRI/XhoI;</t> 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.
    Pgem T Easy Promega Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy promega vector/product/Promega
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    pgem t easy promega vector - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: pGEM-T/EcoRI/XhoI; 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.

    Journal:

    Article Title: Cloning and molecular characterization of Δ12-fatty acid desaturase gene from Mortierella isabellina

    doi: 10.3748/wjg.v12.i21.3373

    Figure Lengend Snippet: Identification of the recombinant plasmid, pTMICL12 by restriction enzyme. 1: pGEM-T/EcoRI/XhoI; 2: DNA size marker λ DNA/BstII; 3: pTMICL12/EcoRI/XhoI; 4: PCR product of D12D DNA.

    Article Snippet: PCR product about 1.2 kb long was gel-purified by electrophoresis and cloned into pGEM-T vector.

    Techniques: Recombinant, Plasmid Preparation, Marker, Polymerase Chain Reaction

    Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector pGEM-T in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).

    Journal: Infection and Immunity

    Article Title: Molecular Characterization of Mycoplasma arthritidis Variable Surface Protein MAA2

    doi:

    Figure Lengend Snippet: Expression of recombinant MAA2 in E. coli . (A) E. coli cells containing the maa2 gene ligated into vector pGEM-T in both orientations with respect to the lacZ promoter were grown in the presence and absence of IPTG, lysed, electrophoresed by SDS-PAGE on 7.5% (wt/vol) gels, transferred to nitrocellulose, and stained with MAA2-specific MAb 7a. Lanes contain M. arthritidis 158p10p9 whole-cell lysate (lane 1), E. coli containing the cloned insert in the forward orientation with respect to the lacZ promoter, in the absence (lane 2) and presence (lane 3) of IPTG, E. coli containing the cloned insert in the reverse orientation with respect to the lacZ promoter, in the absence (lane 4) and presence (lane 5) of IPTG, and untransformed E. coli in the absence (lane 6) and presence (lane 7) of IPTG. (B) E. coli cells containing the maa2 gene from M. arthritidis 158p10p9 clonal variants in which expression of MAA2 was switched ON or OFF were grown in the presence or absence of IPTG, lysed, electrophoresed and immunoblotted as described above. Lanes contain E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the reverse orientation with respect to the lacZ promoter, in the absence (lane 1) and presence (lane 2) of IPTG, E. coli in which maa2 from an OFF variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 3) and presence (lane 4) of IPTG, E. coli in which maa2 from an ON variant was ligated into vector pGEM-T in the forward orientation with respect to the lacZ promoter, in the absence (lane 5) and presence (lane 6) of IPTG, and a whole-cell lysate of M. arthritidis strain 158p10p9 (lane 7).

    Article Snippet: For sequencing through the repeat regions of maa2 , sequences amplified by PCR from forward and reverse primers NT and HMPR were cloned into vector pGEM-T as described above, and a series of nested deletions was prepared by using the Erase-A-Base system (Promega).

    Techniques: Expressing, Recombinant, Plasmid Preparation, SDS Page, Staining, Clone Assay, Variant Assay

    Trans -acting factors specifically binding to the editing sites in the extracts of tobacco chloroplasts. ( A ) UV-crosslinking was performed with a respective RNA probe that was labeled with 32 P at +1 (C to be edited). Lanes 1, without competitor RNA; lanes 2, a 100-fold molar excess of unlabeled probe RNA was added as a competitor; lanes 3, a 100-fold molar excess of control RNA that was a 161 nt transcript of a pGEM-T vector was added as a competitor. Free indicates the bands of a free probe that migrated in front of the protein bands on SDS–PAGE. ( B ) Comparison of the electrophoretic mobilities of p95s binding to ndhB-9 (lane 1) and ndhF-1 (lane 2).

    Journal: Nucleic Acids Research

    Article Title: Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts

    doi: 10.1093/nar/gkm1026

    Figure Lengend Snippet: Trans -acting factors specifically binding to the editing sites in the extracts of tobacco chloroplasts. ( A ) UV-crosslinking was performed with a respective RNA probe that was labeled with 32 P at +1 (C to be edited). Lanes 1, without competitor RNA; lanes 2, a 100-fold molar excess of unlabeled probe RNA was added as a competitor; lanes 3, a 100-fold molar excess of control RNA that was a 161 nt transcript of a pGEM-T vector was added as a competitor. Free indicates the bands of a free probe that migrated in front of the protein bands on SDS–PAGE. ( B ) Comparison of the electrophoretic mobilities of p95s binding to ndhB-9 (lane 1) and ndhF-1 (lane 2).

    Article Snippet: The amplified fragments were cloned into a pGEM-T vector using the pGEM-T Vector System (Promega).

    Techniques: Binding Assay, Labeling, Plasmid Preparation, SDS Page