Journal: Cell & Bioscience
Article Title: Kv1.3 channel blocker (ImKTx88) maintains blood–brain barrier in experimental autoimmune encephalomyelitis
Figure Lengend Snippet: Effects of ImKTx88 on ICAM-1 and VCAM-1 expression. a Representative immunohistochemistry of ICAM-1 and VCAM-1 expression in the cerebellar white matter of EAE-induced and ImKTx88 treated rats. The scale bar represents 100 µm. Staining was quantified by mean of the integrated optical density (IOD) (5 random images per section and n = 3). b A representative western blot of cerebellum homogenates and quantification analysis revealed that the levels of ICAM-1 and VCAM-1 were significantly increased in EAE-induced rats, and ImKTx88 could decrease the levels of ICAM-1 and VCAM-1 (n = 5–12). GAPDH was used as an internal loading control. Data represent the mean ± SEM, * P
Article Snippet: Blots were incubated overnight with the primary antibodies as follows: occludin (1:1000, Abcam, Cambridge, MA, USA); ZO-1 (1:500, Invitrogen, Carlsbad, CA, USA); claudin-5 (1:500, Abclonal, Woburn, MA, USA); ICAM-1(1:1000, Abclonal, Woburn, MA, USA); VCAM-1 (1:1000, Abclonal, Woburn, MA, USA); Ang-1 (1:500, Abclonal, Woburn, MA, USA); Tie-2 (1:500, Abclonal, Woburn, MA, USA); GAPDH (1:5000, Abclonal, Woburn, MA, USA).
Techniques: Expressing, Immunohistochemistry, Staining, Western Blot