variable heavy (vh) sequences Search Results


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GenScript corporation variable region sequences of light chain antibody
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GenScript corporation hybridoma heavy and light chain variable sequences and imgt analysis
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Hybridoma Heavy And Light Chain Variable Sequences And Imgt Analysis, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AbbVie Inc sequences of the variable regions of the heavy and light chains
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Sequences Of The Variable Regions Of The Heavy And Light Chains, supplied by AbbVie Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation chikv fab cap101a.e8 variable heavy and light chain cdnas bearing human il-2 signal sequences
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Chikv Fab Cap101a.E8 Variable Heavy And Light Chain Cdnas Bearing Human Il 2 Signal Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProMab Inc sequencing of the variable heavy chain
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Sequencing Of The Variable Heavy Chain, supplied by ProMab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation antigen binding sites (the variable regions of light (vl) and heavy (vh) chains
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Antigen Binding Sites (The Variable Regions Of Light (Vl) And Heavy (Vh) Chains, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LakePharma heavy- and light-chain variable region sequences
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Heavy And Light Chain Variable Region Sequences, supplied by LakePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation gene coding sequences for vh and vl chain variable regions of humanized and chimeric jaa-f11
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Gene Coding Sequences For Vh And Vl Chain Variable Regions Of Humanized And Chimeric Jaa F11, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iRepertoire Inc amplification of ig heavy variable (vh) and light variable (vl) domains
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Amplification Of Ig Heavy Variable (Vh) And Light Variable (Vl) Domains, supplied by iRepertoire Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NextGen Sciences sequencing of the immunoglobulin heavy chain variable region (v h gene) repertoire
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Sequencing Of The Immunoglobulin Heavy Chain Variable Region (V H Gene) Repertoire, supplied by NextGen Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation nucleotide sequences encoding the heavy chain variable region and the light chain variable region
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Nucleotide Sequences Encoding The Heavy Chain Variable Region And The Light Chain Variable Region, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from hybridoma supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

Journal: Structure (London, England : 1993)

Article Title: Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability

doi: 10.1016/j.str.2022.06.004

Figure Lengend Snippet: Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from hybridoma supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also Figure S1 .

Article Snippet: Select hybridomas were sent to GenScript (Piscataway, NJ 08854, USA) for hybridoma heavy and light chain variable sequences and IMGT ( ) analysis.

Techniques: Sequencing, Binding Assay, Isolation, Enzyme-linked Immunosorbent Assay, Purification, Bioprocessing, Neutralization