Journal: ACS Omega

Article Title: Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin

doi: 10.1021/acsomega.3c08113

Figure Lengend Snippet: Microscopic characterization of nanofibrous mat SEM images of formulations of vancomycin nanofibers at magnifications of 5000x and corresponding size distribution histograms. (A) Formulation with chitosan in HFIP. (B) Formulation with chitosan and PVA in acetic acid solution (F2). (C) Formulation with chitosan and PEO in acetic acid solution (F4). Chitosan alone proved to be extremely difficult to spin due to its limited solubility (A), and for this reason, the addition of a copolymer such as PVA and PEO was explored to enhance the electrospinnability properties of chitosan (B,C).

Article Snippet: Vancomycin HCl was purchased from Tocris Bioscience (Minneapolis).

Techniques: Formulation, Solubility

Journal: eLife

Article Title: The gut microbiota is a transmissible determinant of skeletal maturation

doi: 10.7554/eLife.64237

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , Vancomycin , Selleckchem , Cat# S2575 , For Microbiota depletion.

Techniques: In Vivo, Isolation, Activation Assay, cDNA Synthesis, SYBR Green Assay, Software, Flow Cytometry

Journal: Pharmaceutics

Article Title: Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections

doi: 10.3390/pharmaceutics13122151

Figure Lengend Snippet: ( A ) Images of pure 3% alginate and of clindamycin, gentamicin, and vancomycin in 3% alginate at a concentration of 10 mg/mL (positioned left to right, respectively). ( B ) Images of pure 3% Laponite and of clindamycin, gentamicin, and vancomycin in 3% Laponite at a concentration of 10 mg/mL (positioned left to right, respectively). After adding 20 mg/mL antibiotic solution to 6% Laponite and stirring, a clear phase separation (after a few minutes at rest) of clindamycin and gentamicin was observed.

Article Snippet: Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH 2 O at a concentration of 20 mg/mL.

Techniques: Concentration Assay

Journal: Pharmaceutics

Article Title: Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections

doi: 10.3390/pharmaceutics13122151

Figure Lengend Snippet: Rheological characterization of core and shell biomaterial inks. Representative plots showing the shear thinning behaviour of core—3% alginate and 3% Laponite loaded with antibiotics ( A , C ) and of shell—ALG-MC and ALG-MC-LAP ( E ). Viscosity of core and shell biomaterial inks, measured at a constant shear rate of 10 s −1 over a period of 100 s ( B , D , F ), respectively.

Article Snippet: Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH 2 O at a concentration of 20 mg/mL.

Techniques: Shear, Viscosity

Journal: Pharmaceutics

Article Title: Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections

doi: 10.3390/pharmaceutics13122151

Figure Lengend Snippet: Spectrophotometric quantification of antibiotics. Standard curves ( A , C , E ) obtained by measuring the absorbance at 198 nm, 230 nm and Ninhydrin assay of defined concentrations of clindamycin, vancomycin and gentamicin in 0.9% NaCl solution, respectively. Analysis of the release solutions ( B , D , F ) obtained from antibiotic loaded and unloaded (control) ALG-MC scaffolds, using the respective method ( n = 5).

Article Snippet: Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH 2 O at a concentration of 20 mg/mL.

Techniques: Control

Journal: Pharmaceutics

Article Title: Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections

doi: 10.3390/pharmaceutics13122151

Figure Lengend Snippet: Adaptation of the agar diffusion test for quantification of antibiotics. A schematic diagram of an agar plate with sample carriers: Numbers in each sector indicate the concentration of antibiotic solution. Example images of the agar plates whose sample carriers were loaded with vancomycin, which led to formation of a clear area (ZOI) around the sample carriers ( A ). Standard curves of clindamycin, gentamicin and vancomycin obtained by plotting the area of ZOI developed on agar plates containing S. aureus ( B ) and S. epidermidis ( C ) vs. respective antibiotic concentration loaded on the sample carriers ( n = 3).

Article Snippet: Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH 2 O at a concentration of 20 mg/mL.

Techniques: Diffusion-based Assay, Concentration Assay

Journal: Pharmaceutics

Article Title: Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections

doi: 10.3390/pharmaceutics13122151

Figure Lengend Snippet: CEC of antibiotics from C/S scaffolds; impact of shell biomaterial ink composition. Cumulative CEC release of antibiotics loaded in ALG-MC and ALG-MC-LAP C/S scaffolds (printed with 200/840 C/S needles), quantified by agar diffusion assay (using S. aureus strain; A , C , E ) over a period of 7 d. Statistical analysis performed only on burst release, i.e., release of antibiotics at 2 h ( n = 3; * p < 0.05; *** p < 0.001). Representative images of ZOI formed on the agar plate when release solutions were added to sample carriers ( B , D , F ). A cropped image of ZOI of d 7 release sample of gentamicin was performed on a different agar plate and is added onto the ZOI of remaining samples, * in ( F ).

Article Snippet: Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH 2 O at a concentration of 20 mg/mL.

Techniques: Diffusion-based Assay

Journal: Pharmaceutics

Article Title: Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections

doi: 10.3390/pharmaceutics13122151

Figure Lengend Snippet: Release of vancomycin from C/S scaffolds: impact of shell thickness. Schematic diagram showing different coaxial needle combinations ( A ). Release of vancomycin from ALG-MC scaffolds having different shell thickness ( n = 5; ** p < 0.005) ( B ). Statistical analysis performed only on burst release, i.e., release of antibiotics at 2 h ( n = 3; ** p < 0.005). Representative images of ZOI formed in the presence of release solutions collected from scaffolds with different shell thickness after 2 h and 6 h, respectively ( C; release solutions obtained from three scaffolds at respective time point were loaded on the sample carrier, denoted by I, II, III).

Article Snippet: Respective antibiotic powders (Vancomycin HCl, Clindamycin HCl and Gentamicin sulphate, all provided by Heraeus Medical, Germany) were first dissolved in ddH 2 O at a concentration of 20 mg/mL.

Techniques: