Journal: Epigenetics

Article Title: DNA methylation profiling identifies a high effect genetic variant for lipoprotein(a) levels.

doi: 10.1080/15592294.2020.1739797

Figure Lengend Snippet: Figure 1. Lp(a) EWAS in the discovery cohort: (a) Manhattan and (b) QQ plot. Plasma Lp(a) EWAS (rank regression), adjusted for age, sex, and blood cell composition. Only one CpG site, cg17028067 (red circle), was significantly associated with Lp(a) after multiple testing correction (p < 4 × 10−8, Q < 0.05).

Article Snippet: Genotyping of rs76735376 by Sanger sequencing and TaqMan assay The 294-bp DNA region surrounding cg17028067 (−212/+81 bp; Supplementary Table 1) was amplified from the genomic DNA of 59 individuals that had cg17028067 β-values of <0.55 and 135 that had β-values of >0.74 using Platinum® Taq DNA Polymerase (Life Technologies, CA, USA) and the primers listed in Supplementary Table 1.

Techniques: Clinical Proteomics

Journal: Epigenetics

Article Title: DNA methylation profiling identifies a high effect genetic variant for lipoprotein(a) levels.

doi: 10.1080/15592294.2020.1739797

Figure Lengend Snippet: Figure 2. The relationship between plasma Lp(a), cg1702867 methylation, and rs76735376 genotype in the discovery cohort. (a) Note that the majority (80%) of those individuals with cg17028067 β values below 0.5 were heterozygotes for rs76735376. (b) Rs76735376 heterozygotes had significantly higher plasma Lp(a) levels than major allele homozygotes (p < 0.0001, Mann–Whitney U-test).

Article Snippet: Genotyping of rs76735376 by Sanger sequencing and TaqMan assay The 294-bp DNA region surrounding cg17028067 (−212/+81 bp; Supplementary Table 1) was amplified from the genomic DNA of 59 individuals that had cg17028067 β-values of <0.55 and 135 that had β-values of >0.74 using Platinum® Taq DNA Polymerase (Life Technologies, CA, USA) and the primers listed in Supplementary Table 1.

Techniques: Clinical Proteomics, Methylation, MANN-WHITNEY

Journal: Epigenetics

Article Title: DNA methylation profiling identifies a high effect genetic variant for lipoprotein(a) levels.

doi: 10.1080/15592294.2020.1739797

Figure Lengend Snippet: Figure 3. Box and Whisker plots of significant plasma Lp(a) SNP associations in a New Zealand cohort. (a) The SNP associated with the cg17028067 methylation site, rs76735376, compared with other New Zealand cohort top hit Lp(a) SNPs (b–g) that had been associated with Lp(a) in previous meta-GWAS reports [11,12]. (h) The methylation site-associated SNP was not in strong linkage disequilibrium with any of these SNPs. (i) SNP positions relative to the LPA gene (rs76735376 indicated by a red box).

Article Snippet: Genotyping of rs76735376 by Sanger sequencing and TaqMan assay The 294-bp DNA region surrounding cg17028067 (−212/+81 bp; Supplementary Table 1) was amplified from the genomic DNA of 59 individuals that had cg17028067 β-values of <0.55 and 135 that had β-values of >0.74 using Platinum® Taq DNA Polymerase (Life Technologies, CA, USA) and the primers listed in Supplementary Table 1.

Techniques: Whisker Assay, Clinical Proteomics, Methylation

Journal:

Article Title: Combining Nerve Fiber Layer to Optimize Glaucoma Diagnosis with Optical Coherence Tomography

doi: 10.1016/j.ophtha.2008.01.011

Figure Lengend Snippet: Sensitivity and Specificity Analyses for Retinal Nerve Fiber Layer (RNFL) Parameters Based on the Stratus Age-Adjusted Normative Reference

Article Snippet: Stratus age-adjusted normative reference cutoff values at 1 and 5 percentile points provided by Jennifer Lipyanik, senior clinical research scientist, Carl Zeiss Meditec.

Techniques: