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Image Search Results
Journal: Parasites & Vectors
Article Title: Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany
doi: 10.1186/s13071-018-3240-7
Figure Lengend Snippet: Workflow of all samples. DNA of ticks and animal blood samples was extracted and PCRs for Bartonella -specific genes ( 16S rDNA, 16S-23S ITS) were conducted (with subsequent Sanger-sequencing of the amplicons). 16S rDNA metagenomics was used for determination of the tick microbiome (confirmed by specific PCRs) revealing the presence of further pathogens. Serum of pets and, if available, of pet owners was analyzed for serological infection markers (antibodies) known to indicate previous infections in regard to the molecular findings from ticks
Article Snippet: The workflow is summarized in Fig. . 16S full length rRNA gene sequencing data were analyzed applying the EPI2ME platform with
Techniques: Sequencing, Infection
Journal: Parasites & Vectors
Article Title: Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany
doi: 10.1186/s13071-018-3240-7
Figure Lengend Snippet: Targets, primers and amplicon size for the PCR-testing from ticks and EDTA-blood
Article Snippet: The workflow is summarized in Fig. . 16S full length rRNA gene sequencing data were analyzed applying the EPI2ME platform with
Techniques: Amplification, Sequencing
Journal: Parasites & Vectors
Article Title: Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany
doi: 10.1186/s13071-018-3240-7
Figure Lengend Snippet: Schematic overview of microbiome bioinformatic analysis workflow. The hypervariable V4 region of 16S rDNA from tick samples was sequenced and split by barcode with Illumina MiSeq. Resulting paired-end reads were joined and the primer region was removed. Reads were filtered by amplicon length and aligned to SILVA as the reference database. After removal of chimeras, reads were clustered into operational taxonomic units (OTU) and taxonomically classified. Finally, an OTU-table was created and results were visualized
Article Snippet: The workflow is summarized in Fig. . 16S full length rRNA gene sequencing data were analyzed applying the EPI2ME platform with
Techniques: Amplification