Journal: iScience

Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging

doi: 10.1016/j.isci.2022.105779

Figure Lengend Snippet:

Article Snippet: 3xFlag-HaloTag-EZH2 HDR - pDY053 , Youmans et al. , Addgene: 171108.

Techniques: Virus, Recombinant, Western Blot, Software, Modification, DNA Extraction

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A , B The expression of ANXA7 and SRI in different cancers or specific cancer subtypes was analyzed through TIMER2. The expression of ANXA7 and SRI in HCC are higher ( n = 371) than normal liver tissues ( n = 50) (*** p < 0.001). C , D The expression of ANXA7 is higher in HCC ( n = 369) than in normal liver tissues ( n = 160) from the TCGA database. The expression of SRI is also higher in HCC ( n = 369) than in normal liver tissues ( n = 160) from the TCGA database ( * p < 0.05). E – G The expression of ANXA7 and SRI were higher in Huh-7/Hep3B cells than in normal human hepatocytes HL-7702 by qRT-PCR and western blotting. H Representative images of ANXA7 and SRI expression in normal human liver tissues and HCC tissues. I Quantification of the average means optical density for ANXA7 and SRI in normal liver tissues and HCC tissues (** p < 0.01, * p < 0.05). J ANXA7 expression level p ositively correlated with SRI expression in HCC cells ( r 2 = 0.882, p < 0.01). K ANXA7 expression level positively correlated with SRI expression in HCC tissues ( r 2 = 0.707, p < 0.01).

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A There may be a potential interaction between ANXA7 and SRI, which was analyzed by the STRING database. B co-IP assay showed the interaction between ANXA7 and SRI in Huh-7/Hep3B cells. C Cellular immunofluorescence proved that ANXA7 and SRI are colocalized in the cytoplasm and cell membrane in Huh-7/Hep3B cells.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Co-Immunoprecipitation Assay, Immunofluorescence, Membrane

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A – D Upregulated ANXA7 increased SRI expression and downregulated ANXA7 decreased SRI expression at the mRNA level in Huh-7 and Hep3B cells (** p < 0.01,* p < 0.05). E – H Upregulated ANXA7 increased SRI expression at the protein level and promoted EMT, whereas downregulated ANXA7 decreased SRI expression at the protein level and inhibited EMT in Huh-7 and Hep3B cells (** p < 0.01,* p < 0.05). I – J IF was used to detect E-cadherin and cytokeratin. K , L IF assay was used to detect the N-cadherin and Vimentin in Huh-7/Hep3B cells.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A , B Images of tumor xenografts and tumor weight (** p < 0.01). C , D The qRT-PCR analysis of ANXA7 and SRI in tumor xenografts (** p < 0.01). E , F Western blotting to detect ANXA7, SRI, E-cadherin, Cytokeratin, N-cadherin, and Vimentin in tumor xenografts (** p < 0.01, * p < 0.05). G , H Immunohistochemical analysis of ANXA7, SRI and EMT indicators in tumor xenografts (** p < 0.01, * p < 0.05).

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemical staining

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A – D When SRI is upregulated, the expression of ANXA7 increased significantly, while when SRI is downregulated, the expression of ANXA7 also decreased in Huh-7 and Hep3B cells by qRT-PCR (** p < 0.01, * p < 0.05). E – H When SRI is upregulated, the expression of ANXA7 increased, and promoted EMT, whereas when SRI is downregulated, the expression of ANXA7 also decreased, and inhibited EMT in Huh-7 and Hep3B cells by western blotting (** p < 0.01, * p < 0.05). I , J IF was used to detect the E-cadherin and Cytokeratin after upregulating and downregulating SRI. K , L IF was used to detect the N-cadherin and Vimentin in Huh-7/Hep3B cells after upregulating and downregulating SRI.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: Interaction between ANXA7 and SRI affects EMT progress and contributes to the aggressiveness in HCC.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques:

Journal: Scientific Reports

Article Title: RETRACTED ARTICLE: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma

doi: 10.1038/s41598-017-10365-3

Figure Lengend Snippet: Sorcin promotes HCC cell invasion and metastasis in vitro and in vivo . (A&B) Sorcin promoted HCC cell migration and invasion in vitro . Transwell migration ( A ) and invasion ( B ) assays of HepG2 cells with Sorcin overexpression (HepG2 Sorcin ) or control (HepG2 Vector ), and HCCLM3 cells with Sorcin knockdown (HCCLM3 shSorcin ) or control (HCCLM3 shcontrol ). ( C , D ) Sorcin promoted HCC cell growth in vivo . ( C ) Left column: Linear graphs were used to analyze the rate of tumor growth in each group. Middle column: Representative subcutaneous tumors from HepG2 Sorcin and HCCLM3 shSorcin cells and their control cells were shown in the middle panel. Right column: Tumor volumes of each group were shown and compared in the bar charts. ( D ) Left and middle column: Representative pictures of orthotopic liver exnograft tumors from each indicated groups were shown. Right column: Tumor volumes of each group were shown and compared in the bar charts. ( E ) Representative pictures of intrahepatic ( E1 ) and lung metastasis ( E2) . original magnification: left, 100×; right, 400×. ( F ) The number of metastatic nodules in each mice liver ( F1 ) or lung ( F2 ) was calculated and compared. *** P < 0.001. Error bars, SD.

Article Snippet: The lentiviral vectors (LV) encoding short hairpin RNAs (shRNAs) for Sorcin knockdown, and LV encoding Sorcin gene were purchased from OriGene Technologies Incorporation (Rockville, MD).

Techniques: In Vitro, In Vivo, Migration, Over Expression, Control, Plasmid Preparation, Knockdown

Journal: Scientific Reports

Article Title: RETRACTED ARTICLE: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma

doi: 10.1038/s41598-017-10365-3

Figure Lengend Snippet: Sorcin Enhances Metastasis by Activating ERK Signaling via Facilitating EMT. ( A ) Representative phase contrast images for cell morphology showed that Sorcin affected the HCC cell morphology ( A1 ) and F-actin immunofluorescence staining was used to analyze the cytoskeleton in HepG2 Sorcin , HCCLM3 shSorcin and their control cells ( A2 ). ( B ) Immunofluorescent (IF) showed the relative expression of E-cadherin (red), Vimentin (green) in HepG2 Sorcin with Sorcin overexpression or HCCLM3 shSorcin cells with Sorcin knockdown, their corresponding control cells. ( C ) Western blot analysis of E-cadherin and vimentin expressions in HepG2 Sorcin , HCCLM3 shSorcin and their control cells with/without U0126 treatment. ( D ) Representative IHC images of Sorcin, p-ERK, E-cadherin and Vimentin expression in HCC tissues. Magnification: 100×.

Article Snippet: The lentiviral vectors (LV) encoding short hairpin RNAs (shRNAs) for Sorcin knockdown, and LV encoding Sorcin gene were purchased from OriGene Technologies Incorporation (Rockville, MD).

Techniques: Immunofluorescence, Staining, Control, Expressing, Over Expression, Knockdown, Western Blot