v12 Search Results


stata version 12  (STATA Corporation)
99
STATA Corporation stata version 12
Stata Version 12, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/10__1080_slash_19439342__2014__974200-150-5-5?v=STATA+Corporation
Average 99 stars, based on 1 article reviews
stata version 12 - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
epifluorescence stereomicroscope  (Carl Zeiss)
96
Carl Zeiss epifluorescence stereomicroscope
Epifluorescence Stereomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pm40393452-248-17-21?v=Carl+Zeiss
Average 96 stars, based on 1 article reviews
epifluorescence stereomicroscope - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
pbabe puro h ras v12  (Addgene inc)
94
Addgene inc pbabe puro h ras v12
Pbabe Puro H Ras V12, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/bio_rxiv__64898__2026__01__13__699211-236-18-22?v=Addgene+inc
Average 94 stars, based on 1 article reviews
pbabe puro h ras v12 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
fluorescence dissection stereomicroscope  (Carl Zeiss)
94
Carl Zeiss fluorescence dissection stereomicroscope
Fluorescence Dissection Stereomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pmc07213980-294-25-28?v=Carl+Zeiss
Average 94 stars, based on 1 article reviews
fluorescence dissection stereomicroscope - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
stereo discovery v12 microscope  (Carl Zeiss)
96
Carl Zeiss stereo discovery v12 microscope
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
Stereo Discovery V12 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pmc09523645-55-6-5?v=Carl+Zeiss
Average 96 stars, based on 1 article reviews
stereo discovery v12 microscope - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
stereomicroscope  (Carl Zeiss)
96
Carl Zeiss stereomicroscope
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
Stereomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pmc12432336-78-5-6?v=Carl+Zeiss
Average 96 stars, based on 1 article reviews
stereomicroscope - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
pwzl h rasv12  (Addgene inc)
92
Addgene inc pwzl h rasv12
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
Pwzl H Rasv12, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pmc09565228__pnas__2208255119__sapp-48-30-31?v=Addgene+inc
Average 92 stars, based on 1 article reviews
pwzl h rasv12 - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
ras ires gfp expression vector  (Addgene inc)
93
Addgene inc ras ires gfp expression vector
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
Ras Ires Gfp Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pmc06246920-451-0-6?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ras ires gfp expression vector - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
g12v mutant kras  (Addgene inc)
93
Addgene inc g12v mutant kras
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
G12v Mutant Kras, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/us09938582-869-3-6?v=Addgene+inc
Average 93 stars, based on 1 article reviews
g12v mutant kras - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
kras g12v  (Addgene inc)
93
Addgene inc kras g12v
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
Kras G12v, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pmc05854482__NIHMS949230___supplement___3-349-4-6?v=Addgene+inc
Average 93 stars, based on 1 article reviews
kras g12v - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
p babe hras v  (Addgene inc)
93
Addgene inc p babe hras v
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
P Babe Hras V, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/bio_rxiv__2025__10__30__685565-224-35-40?v=Addgene+inc
Average 93 stars, based on 1 article reviews
p babe hras v - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
paul melançon  (Addgene inc)
91
Addgene inc paul melançon
a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO <t>Discovery.V12</t> microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.
Paul Melançon, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v12/pm37289133-348-21-29?v=Addgene+inc
Average 91 stars, based on 1 article reviews
paul melançon - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier

Image Search Results


a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO Discovery.V12 microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.

Journal: Communications Biology

Article Title: SARS-CoV-2 Nsp6 damages Drosophila heart and mouse cardiomyocytes through MGA/MAX complex-mediated increased glycolysis

doi: 10.1038/s42003-022-03986-6

Figure Lengend Snippet: a Schematic representation of the genetic screen to identify individual SARS-CoV-2 genes with cardiac pathology. Fly images taken using a ZEISS SteREO Discovery.V12 microscope. Scale bar = 0.5 mm. b Quantitation of mortality rate prior to eclosion for each individually expressed SARS-CoV-2 gene from the crosses in a . Mortality was calculated as (CyO wing − straight wing)/CyO wing × 100. n = ~200 flies (across 4 vials). c Graph displaying the lifespan for adult flies carrying SARS-CoV-2 Nsp6 , Orf6 , Orf7a or Nsp3 transgene expression. w 1118 is a wild-type control. d The adult heart phenotype induced by 4X Hand -Gal4, cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. Cardiac actin myofibers were visualized by phalloidin staining. Dotted lines delineate the outline of the heart tube. Arrow points to missing cardiac myofibers. w 1118 is a wild-type control. Scale bars (both) = 40 μm. e Quantitation of adult heart cardiac myofibrillar density relative to control. SARS-CoV-2 Nsp6 (4X Hand -Gal4) flies, and w 1118 is a wild-type control. P value = 5.3E−05. n = 6 flies per genotype. f Schematic representation of heart diastolic and systolic diameter, and the heart period by optical coherence tomography (OCT). Scale bars (top two panels) = 20 μm; (bottom) = 30 μm. g Drosophila heartbeat video images from flies induced by 4X Hand - Gal4 cardioblast-specific overexpression (OE) of the UAS- SARS-CoV-2 Nsp6 transgene. w 1118 is a wild-type control. Scale bars (both) = 25 μm. h Quantitation of adult heart diastolic diameter. n = 10 flies per genotype. P value = 8.3E−08. i Quantitation of heart period, i.e., indication of heart rate. n = 10 flies per genotype. P value = 4.0E−08. Statistical significance (*) defined as P < 0.05 (Student’s t test); data shown as mean ± SD.

Article Snippet: Fly images taken using a ZEISS SteREO Discovery.V12 microscope.

Techniques: Microscopy, Quantitation Assay, Expressing, Over Expression, Staining, Tomography