Journal: International Journal of Molecular Sciences

Article Title: Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv

doi: 10.3390/ijms22073547

Figure Lengend Snippet: ( A ) 3D model of gp41 (PDB ID 2X7RC and 2MF1A , brown) and scFv from trastuzumab (PDB ID 3AUVA , blue) (inset: distance between Cα of the C-terminal of scFv and ASP12 of the MPER of gp41); ( B ) new sequence-based protein structure model. The transmembrane region (TM) is identified by the grey region.

Article Snippet: Taken all together, our main aim was the design and production of a biomimetic vector containing the scFv of trastuzumab, a well-known anti-HER2 antibody, as a targeting motif fused with HIV viral protein gp41.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv

doi: 10.3390/ijms22073547

Figure Lengend Snippet: Constructed DNA plasmid encoding the scFv-HER2_gp41 sequence.

Article Snippet: Taken all together, our main aim was the design and production of a biomimetic vector containing the scFv of trastuzumab, a well-known anti-HER2 antibody, as a targeting motif fused with HIV viral protein gp41.

Techniques: Construct, Plasmid Preparation, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv

doi: 10.3390/ijms22073547

Figure Lengend Snippet: Western blot analysis. Lane 1: protein ladder, lane 2: negative control (transfection without a plasmid), lane 3: scFv-HER2_gp41 protein (transfection with plasmid X1665). Western blot detection was performed using an HRP-conjugated anti-HA polyclonal antibody (1:2500). Visualization of bands was carried out using the ECL ® reagent.

Article Snippet: Taken all together, our main aim was the design and production of a biomimetic vector containing the scFv of trastuzumab, a well-known anti-HER2 antibody, as a targeting motif fused with HIV viral protein gp41.

Techniques: Western Blot, Negative Control, Transfection, Plasmid Preparation