v.25 Search Results


rab25 shrna lentiviral particles  (Santa Cruz Biotechnology)
86
Santa Cruz Biotechnology rab25 shrna lentiviral particles
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Rab25 Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v%2E25/pmc04012721-191-18-22?v=Santa+Cruz+Biotechnology
Average 86 stars, based on 1 article reviews
rab25 shrna lentiviral particles - by Bioz Stars, 2026-08
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αv mapple plasmid  (Addgene inc)
93
Addgene inc αv mapple plasmid
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
αv Mapple Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zymo spintm columns  (Zymo Research)
95
Zymo Research zymo spintm columns
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Zymo Spintm Columns, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glutaraldehyde  (Thermo Fisher)
93
Thermo Fisher glutaraldehyde
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Glutaraldehyde, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glutaraldehyde - by Bioz Stars, 2026-08
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αv integrin memerald  (Addgene inc)
93
Addgene inc αv integrin memerald
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
αv Integrin Memerald, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pps cfp2 α v integrin  (Addgene inc)
85
Addgene inc pps cfp2 α v integrin
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Pps Cfp2 α V Integrin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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meos2 alpha v integrin n 25  (Addgene inc)
86
Addgene inc meos2 alpha v integrin n 25
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Meos2 Alpha V Integrin N 25, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/v%2E25/bio_rxiv__158618-76-8-6?v=Addgene+inc
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meos2 alpha v integrin n 25 - by Bioz Stars, 2026-08
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chloramphenicol  (Chem Impex International)
95
Chem Impex International chloramphenicol
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Chloramphenicol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minisog alpha v integrin 25  (Addgene inc)
92
Addgene inc minisog alpha v integrin 25
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Minisog Alpha V Integrin 25, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vancomycin  (Chem Impex International)
95
Chem Impex International vancomycin
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Vancomycin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glycerol  (Chem Impex International)
95
Chem Impex International glycerol
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
Glycerol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single crystalline si 100 nanomembranes  (Norcada Inc)
95
Norcada Inc single crystalline si 100 nanomembranes
(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of <t>Rab25</t> expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.
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Image Search Results


(A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of Rab25 expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.

Journal: Oncotarget

Article Title: EGFR endocytosis is a novel therapeutic target in lung cancer with wild-type EGFR

doi:

Figure Lengend Snippet: (A) Difference in endosomal localization of EGF-induced EGFR between H358 and H1703 cells was profiled by immunofuorescence staining with the indicated antibodies. The immunofluorescence images were obtained using a fluorescence microscope at a magnification of 400×. Each white arrow indicates co-localization (yellow). (B) Effect of Rab25 expression on EGFR endocytosis was examined in siRab25 transfected H358 cells by flow cytometry. After siRab25 or scrambled (Scr) transfection, the cells were incubated with gefitinib (10 μM) and the percentage of internalized Alexa Fluor 488-conjugated EGF (500 ng/ml) was analyzed. Each bar represents mean values acquired from three experiments with standard error (SE). * p < 0.05. (C) Correlation between Rab25 expression and gefitinib response was determined by comparative analysis. Expression of Rab25 mRNA was evaluated in eight lung cancer cell lines with wtEGFR using quantitative RT-PCR. Relative Rab25 expression values were normalized to values of GAPDH. IC 50 of each cell was obtained from MTT assay results. The P value represents statistical comparisons between Rab25 expression and gefitinib response. Red: gefitinib-sensitive cell lines. Blue: gefitinib-insensitive cell lines.

Article Snippet: After replacing the culture medium with a Polybrene/media mixture, the cells were incubated with shScrambled control (Scr) and Rab25 shRNA lentiviral particles (Santa Cruz Biotechnology, Santa Cruz, CA) overnight.

Techniques: Staining, Immunofluorescence, Fluorescence, Microscopy, Expressing, Transfection, Flow Cytometry, Incubation, Quantitative RT-PCR, MTT Assay

After siRab25 or scrambled (Scr) transfection, H358 cells were treated with the indicated concentrations of gefitinib. (A) Changed gefitinib response based on Rab25 expression status was monitored by light microscopy. Cell images were captured at a magnification of 100×. Cell viability (B) and IC 50 (C) in siRab25 or Scr transfected cells were determined after gefitinib treatment using MTT assay. Each data point represents mean results of six independent determinations with standard error. * p < 0.05. (D) Redistributed percentages of the cell cycle caused by Rab25 expression status were analyzed after gefitinib treatment using flow cytometry. Data represents mean results acquired from three independent experiments.

Journal: Oncotarget

Article Title: EGFR endocytosis is a novel therapeutic target in lung cancer with wild-type EGFR

doi:

Figure Lengend Snippet: After siRab25 or scrambled (Scr) transfection, H358 cells were treated with the indicated concentrations of gefitinib. (A) Changed gefitinib response based on Rab25 expression status was monitored by light microscopy. Cell images were captured at a magnification of 100×. Cell viability (B) and IC 50 (C) in siRab25 or Scr transfected cells were determined after gefitinib treatment using MTT assay. Each data point represents mean results of six independent determinations with standard error. * p < 0.05. (D) Redistributed percentages of the cell cycle caused by Rab25 expression status were analyzed after gefitinib treatment using flow cytometry. Data represents mean results acquired from three independent experiments.

Article Snippet: After replacing the culture medium with a Polybrene/media mixture, the cells were incubated with shScrambled control (Scr) and Rab25 shRNA lentiviral particles (Santa Cruz Biotechnology, Santa Cruz, CA) overnight.

Techniques: Transfection, Expressing, Light Microscopy, MTT Assay, Flow Cytometry