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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Effects of CANX and DHA on <t>ATP6V0B</t> and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.
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Image Search Results


Effects of CANX and DHA on ATP6V0B and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.

Journal: Oncology Letters

Article Title: Dihydroartemisinin inhibits ATP6 activity, reduces energy metabolism of hepatocellular carcinoma cells, promotes apoptosis and inhibits metastasis via CANX

doi: 10.3892/ol.2024.14607

Figure Lengend Snippet: Effects of CANX and DHA on ATP6V0B and ATP6V1F expression. (A) Expression of (B) CANX, (C) ATP6V0B and (D) ATP6V1F in HuH-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. (E) Expression of (F) CANX, (G) ATP6V0B and (H) ATP6V1F in Li-7 cells, detected by western blotting after CANX knockout, DHA or CANX overexpression. **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.

Article Snippet: The primary antibodies used were CANX (1:5,000; cat. no. 66903-1-Ig; Proteintech Group, Inc.), ATP6V0B (1:1,000; cat. no. 27671-1-AP; Proteintech Group, Inc.) and ATP6V1F (1:1,000; ab190789; Abcam), β-actin (1:2,000; cat. no. 20536-1-AP; Proteintech Group, Inc.).

Techniques: Expressing, Western Blot, Knock-Out, Over Expression, Negative Control, Small Interfering RNA