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uv illuminator illumatool  (Lightools Research)
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Lightools Research uv illuminator illumatool
HCC accessibility to lectin and binding specificity to CSG peptide and TNFα-CSG. (A) Lycopersicon Esculentum (Tomato) Lectin DyLight ® 594 was injected i.v. in mice bearing ALB-Tag HCC or RIP1-Tag5 tumors. Representative micrographs on indicated tissues show lectin-painted vessels (red) in tumors (T) stained with anti-SV40 Large T antigen antibody (green). (B) Mice bearing ALB-Tag HCC were i.v. injected with 0.1 mmol of FAM-CSG, and tissues were collected after 1 h of circulation. Photographic image of collected tissues under bright light (left) and UV <t>illuminator</t> (right) are shown. Peptide binding is shown in tumors (green) but not in normal tissues. The kidney is the clearance organ. (C) HCC tumors from ALB-Tag mice and normal liver from C3H mice were co-stained for laminin and nidogen-1. Representative micrographs show FAM-CSG binding (green) and laminin or nidogen-1 expression (red). Co-localisation is indicated in yellow. Scale bar 100 µm. (D) Quantitative analysis of nidogen-1 staining per field of each tumor or normal liver, as shown in panel (C) and mean ± SEM (****P < 0.0001 by Student’s t -test). (E) Analysis of FITC-TNFα-CSG and FITC-TNFα binding in vivo detected with anti-FITC antibody (green) and nuclear staining (DAPI) in indicated tissues. Scale bars: 25 μm.
Uv Illuminator Illumatool, supplied by Lightools Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uv+illuminator+illumatool/uv+illuminator+illumatool/pmc08902243-47-17-20
Average 90 stars, based on 1 article reviews
uv illuminator illumatool - by Bioz Stars, 2026-11
90/100 stars
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HCC accessibility to lectin and binding specificity to CSG peptide and TNFα-CSG. (A) Lycopersicon Esculentum (Tomato) Lectin DyLight ® 594 was injected i.v. in mice bearing ALB-Tag HCC or RIP1-Tag5 tumors. Representative micrographs on indicated tissues show lectin-painted vessels (red) in tumors (T) stained with anti-SV40 Large T antigen antibody (green). (B) Mice bearing ALB-Tag HCC were i.v. injected with 0.1 mmol of FAM-CSG, and tissues were collected after 1 h of circulation. Photographic image of collected tissues under bright light (left) and UV illuminator (right) are shown. Peptide binding is shown in tumors (green) but not in normal tissues. The kidney is the clearance organ. (C) HCC tumors from ALB-Tag mice and normal liver from C3H mice were co-stained for laminin and nidogen-1. Representative micrographs show FAM-CSG binding (green) and laminin or nidogen-1 expression (red). Co-localisation is indicated in yellow. Scale bar 100 µm. (D) Quantitative analysis of nidogen-1 staining per field of each tumor or normal liver, as shown in panel (C) and mean ± SEM (****P < 0.0001 by Student’s t -test). (E) Analysis of FITC-TNFα-CSG and FITC-TNFα binding in vivo detected with anti-FITC antibody (green) and nuclear staining (DAPI) in indicated tissues. Scale bars: 25 μm.

Journal: Frontiers in Oncology

Article Title: ECM Depletion Is Required to Improve the Intratumoral Uptake of Iron Oxide Nanoparticles in Poorly Perfused Hepatocellular Carcinoma

doi: 10.3389/fonc.2022.837234

Figure Lengend Snippet: HCC accessibility to lectin and binding specificity to CSG peptide and TNFα-CSG. (A) Lycopersicon Esculentum (Tomato) Lectin DyLight ® 594 was injected i.v. in mice bearing ALB-Tag HCC or RIP1-Tag5 tumors. Representative micrographs on indicated tissues show lectin-painted vessels (red) in tumors (T) stained with anti-SV40 Large T antigen antibody (green). (B) Mice bearing ALB-Tag HCC were i.v. injected with 0.1 mmol of FAM-CSG, and tissues were collected after 1 h of circulation. Photographic image of collected tissues under bright light (left) and UV illuminator (right) are shown. Peptide binding is shown in tumors (green) but not in normal tissues. The kidney is the clearance organ. (C) HCC tumors from ALB-Tag mice and normal liver from C3H mice were co-stained for laminin and nidogen-1. Representative micrographs show FAM-CSG binding (green) and laminin or nidogen-1 expression (red). Co-localisation is indicated in yellow. Scale bar 100 µm. (D) Quantitative analysis of nidogen-1 staining per field of each tumor or normal liver, as shown in panel (C) and mean ± SEM (****P < 0.0001 by Student’s t -test). (E) Analysis of FITC-TNFα-CSG and FITC-TNFα binding in vivo detected with anti-FITC antibody (green) and nuclear staining (DAPI) in indicated tissues. Scale bars: 25 μm.

Article Snippet: Tissues, including tumor, heart, liver, spleen, kidney, vertebrae, lung and pancreas were excised and imaged under a UV illuminator (Illumatool, Lightools Research, CA, USA) for assessment of green fluorescence intensity.

Techniques: Binding Assay, Injection, Staining, Expressing, In Vivo