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  • 92
    Carl Zeiss upright microscope
    Morphotypes and abundant phylotypes from a Baltic corrosive community. a Scanning electron microscopy (SEM) image of a spore-forming curved rod resembling a Sporomusa sporulating cell. b SEM micrograph of tetrads of cocci resembling Methanosarcina and their usual cocci aggregates. SEM was performed at the end of transfer #5 (ca. 3 months). c <t>Epifluorescence</t> micrographs of DAPI-stained cells detached from Fe 0 -granules by shaking and sonication during day 18 of transfer #10. We observed two morphotypes: a banana-shaped rod and diplococci. d The diplococci were Methanosarcina as identified by a specific probe for in situ hybridization (Cy3/red-MX821). e Baltic- Methanosarcina sometimes also formed tetrads and could never be visualized as single cells in these Fe 0 -dependent cultures. f Epifluorescence micrograph of DAPI-stained cells detached from Fe 0 -granules by vigorous shaking during day 60 of transfer #10. Only two morphotypes were observed—banana-shaped rods and cocci joined in large aggregates. g Methanosarcina formed cocci aggregates which could be detected by their natural F 420 -autofluorescence. No other morphotypes of methanogens could be detected. h and i Methanosarcina - aggregates with various morphologies and compactness. j Group-specific qPCR to determine the abundance of Methanosarcina and Sporomusa after 18 and 60 days of incubation on Fe 0
    Upright Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 5462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Carl Zeiss
    Average 92 stars, based on 5462 article reviews
    Price from $9.99 to $1999.99
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    92/100 stars
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    93
    Olympus upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 9261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Olympus
    Average 93 stars, based on 9261 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
    93/100 stars
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    92
    Scientifica upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by Scientifica, used in various techniques. Bioz Stars score: 92/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Scientifica
    Average 92 stars, based on 274 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
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    93
    Leica Microsystems upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 93/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Leica Microsystems
    Average 93 stars, based on 248 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
    93/100 stars
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    91
    Leica Biosystems upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Leica Biosystems
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
    91/100 stars
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    90
    WITec upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by WITec, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/WITec
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
    90/100 stars
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    92
    Helmut Hund GmbH upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by Helmut Hund GmbH, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Helmut Hund GmbH
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
    92/100 stars
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    90
    Motic Group upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Microscope, supplied by Motic Group, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Motic Group
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
    90/100 stars
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    92
    Scientifica upright epi fluorescence upright microscope
    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus <t>BX51</t> upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.
    Upright Epi Fluorescence Upright Microscope, supplied by Scientifica, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright epi fluorescence upright microscope/product/Scientifica
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    upright epi fluorescence upright microscope - by Bioz Stars, 2020-05
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    92
    Nikon upright microscope
    The effects of fluoroacetate (FA) on ganglionic cell density and glial expression of key proteins. A–C and A′–C′ : representative <t>epifluorescence</t> images of S100β immunoreactivity (green, A and A′ ), glial fibrillary acidic protein (GFAP) immunoreactivity (magenta, B and B′ ), and overlay ( C and C′ ) in myenteric ganglia from control whole mounts ( A , B , and C ) and whole mounts exposed to FA ( A′ , B′ , and C′ ). Note that S100β immunoreactivity decreases in glia exposed to FA (green, A′ ) and that GFAP immunoreactivity increases in glia exposed to FA (magenta, B′ ). D : quantification of ganglionic S100β, GFAP, and connexin-43 (Cx43) immunoreactivity, expressed as the fluorescence density [arbitrary fluorescence units per square micrometer (au μm −2 )], and the packing density of myenteric glial and neurons [number of S100β + glia and HuC/D + neurons per square millimeter (mm −2 )] expressed as the percentage of buffer control levels. Scale bar ( C′ ) = 10 μm and applies to A–C and A′–C′ . E : quantification of glial cells coexpressing S100β and GFAP. Measurements were obtained by sampling ganglia from n = 4 animals. * P
    Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 92/100, based on 3293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope/product/Nikon
    Average 92 stars, based on 3293 article reviews
    Price from $9.99 to $1999.99
    upright microscope - by Bioz Stars, 2020-05
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    90
    Nikon 80i upright microscope
    The effects of fluoroacetate (FA) on ganglionic cell density and glial expression of key proteins. A–C and A′–C′ : representative <t>epifluorescence</t> images of S100β immunoreactivity (green, A and A′ ), glial fibrillary acidic protein (GFAP) immunoreactivity (magenta, B and B′ ), and overlay ( C and C′ ) in myenteric ganglia from control whole mounts ( A , B , and C ) and whole mounts exposed to FA ( A′ , B′ , and C′ ). Note that S100β immunoreactivity decreases in glia exposed to FA (green, A′ ) and that GFAP immunoreactivity increases in glia exposed to FA (magenta, B′ ). D : quantification of ganglionic S100β, GFAP, and connexin-43 (Cx43) immunoreactivity, expressed as the fluorescence density [arbitrary fluorescence units per square micrometer (au μm −2 )], and the packing density of myenteric glial and neurons [number of S100β + glia and HuC/D + neurons per square millimeter (mm −2 )] expressed as the percentage of buffer control levels. Scale bar ( C′ ) = 10 μm and applies to A–C and A′–C′ . E : quantification of glial cells coexpressing S100β and GFAP. Measurements were obtained by sampling ganglia from n = 4 animals. * P
    80i Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/80i upright microscope/product/Nikon
    Average 90 stars, based on 538 article reviews
    Price from $9.99 to $1999.99
    80i upright microscope - by Bioz Stars, 2020-05
    90/100 stars
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    85
    Carl Zeiss upright axioplan microscope
    The effects of fluoroacetate (FA) on ganglionic cell density and glial expression of key proteins. A–C and A′–C′ : representative <t>epifluorescence</t> images of S100β immunoreactivity (green, A and A′ ), glial fibrillary acidic protein (GFAP) immunoreactivity (magenta, B and B′ ), and overlay ( C and C′ ) in myenteric ganglia from control whole mounts ( A , B , and C ) and whole mounts exposed to FA ( A′ , B′ , and C′ ). Note that S100β immunoreactivity decreases in glia exposed to FA (green, A′ ) and that GFAP immunoreactivity increases in glia exposed to FA (magenta, B′ ). D : quantification of ganglionic S100β, GFAP, and connexin-43 (Cx43) immunoreactivity, expressed as the fluorescence density [arbitrary fluorescence units per square micrometer (au μm −2 )], and the packing density of myenteric glial and neurons [number of S100β + glia and HuC/D + neurons per square millimeter (mm −2 )] expressed as the percentage of buffer control levels. Scale bar ( C′ ) = 10 μm and applies to A–C and A′–C′ . E : quantification of glial cells coexpressing S100β and GFAP. Measurements were obtained by sampling ganglia from n = 4 animals. * P
    Upright Axioplan Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright axioplan microscope/product/Carl Zeiss
    Average 85 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    upright axioplan microscope - by Bioz Stars, 2020-05
    85/100 stars
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    90
    Carl Zeiss axioexaminer upright microscope
    The effects of fluoroacetate (FA) on ganglionic cell density and glial expression of key proteins. A–C and A′–C′ : representative <t>epifluorescence</t> images of S100β immunoreactivity (green, A and A′ ), glial fibrillary acidic protein (GFAP) immunoreactivity (magenta, B and B′ ), and overlay ( C and C′ ) in myenteric ganglia from control whole mounts ( A , B , and C ) and whole mounts exposed to FA ( A′ , B′ , and C′ ). Note that S100β immunoreactivity decreases in glia exposed to FA (green, A′ ) and that GFAP immunoreactivity increases in glia exposed to FA (magenta, B′ ). D : quantification of ganglionic S100β, GFAP, and connexin-43 (Cx43) immunoreactivity, expressed as the fluorescence density [arbitrary fluorescence units per square micrometer (au μm −2 )], and the packing density of myenteric glial and neurons [number of S100β + glia and HuC/D + neurons per square millimeter (mm −2 )] expressed as the percentage of buffer control levels. Scale bar ( C′ ) = 10 μm and applies to A–C and A′–C′ . E : quantification of glial cells coexpressing S100β and GFAP. Measurements were obtained by sampling ganglia from n = 4 animals. * P
    Axioexaminer Upright Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axioexaminer upright microscope/product/Carl Zeiss
    Average 90 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    axioexaminer upright microscope - by Bioz Stars, 2020-05
    90/100 stars
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    Image Search Results


    Morphotypes and abundant phylotypes from a Baltic corrosive community. a Scanning electron microscopy (SEM) image of a spore-forming curved rod resembling a Sporomusa sporulating cell. b SEM micrograph of tetrads of cocci resembling Methanosarcina and their usual cocci aggregates. SEM was performed at the end of transfer #5 (ca. 3 months). c Epifluorescence micrographs of DAPI-stained cells detached from Fe 0 -granules by shaking and sonication during day 18 of transfer #10. We observed two morphotypes: a banana-shaped rod and diplococci. d The diplococci were Methanosarcina as identified by a specific probe for in situ hybridization (Cy3/red-MX821). e Baltic- Methanosarcina sometimes also formed tetrads and could never be visualized as single cells in these Fe 0 -dependent cultures. f Epifluorescence micrograph of DAPI-stained cells detached from Fe 0 -granules by vigorous shaking during day 60 of transfer #10. Only two morphotypes were observed—banana-shaped rods and cocci joined in large aggregates. g Methanosarcina formed cocci aggregates which could be detected by their natural F 420 -autofluorescence. No other morphotypes of methanogens could be detected. h and i Methanosarcina - aggregates with various morphologies and compactness. j Group-specific qPCR to determine the abundance of Methanosarcina and Sporomusa after 18 and 60 days of incubation on Fe 0

    Journal: The ISME Journal

    Article Title: Baltic Sea methanogens compete with acetogens for electrons from metallic iron

    doi: 10.1038/s41396-019-0490-0

    Figure Lengend Snippet: Morphotypes and abundant phylotypes from a Baltic corrosive community. a Scanning electron microscopy (SEM) image of a spore-forming curved rod resembling a Sporomusa sporulating cell. b SEM micrograph of tetrads of cocci resembling Methanosarcina and their usual cocci aggregates. SEM was performed at the end of transfer #5 (ca. 3 months). c Epifluorescence micrographs of DAPI-stained cells detached from Fe 0 -granules by shaking and sonication during day 18 of transfer #10. We observed two morphotypes: a banana-shaped rod and diplococci. d The diplococci were Methanosarcina as identified by a specific probe for in situ hybridization (Cy3/red-MX821). e Baltic- Methanosarcina sometimes also formed tetrads and could never be visualized as single cells in these Fe 0 -dependent cultures. f Epifluorescence micrograph of DAPI-stained cells detached from Fe 0 -granules by vigorous shaking during day 60 of transfer #10. Only two morphotypes were observed—banana-shaped rods and cocci joined in large aggregates. g Methanosarcina formed cocci aggregates which could be detected by their natural F 420 -autofluorescence. No other morphotypes of methanogens could be detected. h and i Methanosarcina - aggregates with various morphologies and compactness. j Group-specific qPCR to determine the abundance of Methanosarcina and Sporomusa after 18 and 60 days of incubation on Fe 0

    Article Snippet: To visualize cells, we used an upright epifluorescence microscope from Zeiss (Axioscope A1) equipped with a Cy3 (excitation 549 nm, emission 562 nm), a DAPI (excitation 359 nm, emission 461 nm), and an F420 -filter set (excitation 420 nm, emission 480 nm).

    Techniques: Electron Microscopy, Staining, Sonication, In Situ Hybridization, Real-time Polymerase Chain Reaction, Incubation

    The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus BX51 upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.

    Journal: PLoS ONE

    Article Title: Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers

    doi: 10.1371/journal.pone.0013917

    Figure Lengend Snippet: The laser-scanning, higher-harmonic optical microscope system. The system was adapted from an Olympus FV300 scanning unit combined with an Olympus BX51 upright microscope. The atrial tissue was placed on the translation stage without fixation and stain. BS, beam splitter; CF, color filter; DM, dichroic mirror; PMT, photo-multiplier tube.

    Article Snippet: Laser-scanning, higher-harmonic optical microscopy of atrial tissue This system was adapted from an Olympus FV300 scanning unit combined with an Olympus BX51 upright microscope.

    Techniques: Microscopy, Staining

    The effects of fluoroacetate (FA) on ganglionic cell density and glial expression of key proteins. A–C and A′–C′ : representative epifluorescence images of S100β immunoreactivity (green, A and A′ ), glial fibrillary acidic protein (GFAP) immunoreactivity (magenta, B and B′ ), and overlay ( C and C′ ) in myenteric ganglia from control whole mounts ( A , B , and C ) and whole mounts exposed to FA ( A′ , B′ , and C′ ). Note that S100β immunoreactivity decreases in glia exposed to FA (green, A′ ) and that GFAP immunoreactivity increases in glia exposed to FA (magenta, B′ ). D : quantification of ganglionic S100β, GFAP, and connexin-43 (Cx43) immunoreactivity, expressed as the fluorescence density [arbitrary fluorescence units per square micrometer (au μm −2 )], and the packing density of myenteric glial and neurons [number of S100β + glia and HuC/D + neurons per square millimeter (mm −2 )] expressed as the percentage of buffer control levels. Scale bar ( C′ ) = 10 μm and applies to A–C and A′–C′ . E : quantification of glial cells coexpressing S100β and GFAP. Measurements were obtained by sampling ganglia from n = 4 animals. * P

    Journal: Journal of Neurophysiology

    Article Title: The acute inhibition of enteric glial metabolism with fluoroacetate alters calcium signaling, hemichannel function, and the expression of key proteins

    doi: 10.1152/jn.00507.2016

    Figure Lengend Snippet: The effects of fluoroacetate (FA) on ganglionic cell density and glial expression of key proteins. A–C and A′–C′ : representative epifluorescence images of S100β immunoreactivity (green, A and A′ ), glial fibrillary acidic protein (GFAP) immunoreactivity (magenta, B and B′ ), and overlay ( C and C′ ) in myenteric ganglia from control whole mounts ( A , B , and C ) and whole mounts exposed to FA ( A′ , B′ , and C′ ). Note that S100β immunoreactivity decreases in glia exposed to FA (green, A′ ) and that GFAP immunoreactivity increases in glia exposed to FA (magenta, B′ ). D : quantification of ganglionic S100β, GFAP, and connexin-43 (Cx43) immunoreactivity, expressed as the fluorescence density [arbitrary fluorescence units per square micrometer (au μm −2 )], and the packing density of myenteric glial and neurons [number of S100β + glia and HuC/D + neurons per square millimeter (mm −2 )] expressed as the percentage of buffer control levels. Scale bar ( C′ ) = 10 μm and applies to A–C and A′–C′ . E : quantification of glial cells coexpressing S100β and GFAP. Measurements were obtained by sampling ganglia from n = 4 animals. * P

    Article Snippet: Images were acquired through the 40× (0.75 numerical aperture, PlanFluor) objective of an upright epifluorescence microscope (Eclipse Ni; Nikon, Melville, NY) with a Retiga 2000R camera (QImaging, Surrey, BC, Canada) controlled by QCapture Pro 7.0 (QImaging).

    Techniques: Expressing, Fluorescence, Sampling

    Sox10-PC::G5-tdT transgenic mice are an effective model to study glial [Ca 2+ ] i responses specifically in the enteric nervous system. A : model of our experimental paradigm comparing Fluo-4 and GCaMP5G imaging in the colonic myenteric plexus. Note that Fluo-4 loads both enteric neurons and glia, whereas GCaMP5G expression is isolated to enteric glia in our genetic model. B : representative epifluorescence images showing the specific expression of tdT (red, left ) in S100β-immunoreactive enteric glia (grayscale, middle ) in the colonic myenteric plexus of Sox10-PC::G5-tdT mice (overlay, right ). C : representative still images from a Ca 2+ imaging experiment showing GCaMP5G fluorescence (grayscale, middle 2 ) in enteric glia (identified by tdT fluorescence; red, left ) in a myenteric ganglion from a Sox10-PC::G5-tdT mouse. GCaMP5G fluorescence is low at rest (baseline) and increases robustly when glial [Ca 2+ ] i responses are stimulated by ADP (100 μM; peak). Note that [Ca 2+ ] i responses are limited to tdT-positive glial cells (overlay, right ). D : representative traces of [Ca 2+ ] i levels in enteric glia (black traces) within a myenteric ganglion (averaged response of all glia within ganglion, overlaid in green) from Sox10-PC::G5-tdT mice exposed to an ADP dose-response curve (10 µM, 100 µM, and 1 mM, subsequently). ΔF/F, change in fluorescence. E : representative traces comparing the mean [Ca 2+ ] i responses of myenteric glia with ADP recorded using GCaMP5G fluorescence of n = 20 glia from a single myenteric ganglion from Sox10-PC :: G5-tdT mice (magenta) or traditional Fluo-4 loading of n = 17 glia from a single myenteric ganglion from background control animals (green). F : GCaMP5G fluorescence reports an average peak [Ca 2+ ] i response that is comparable with those reported by Fluo-4. Peak responses are the average of all glia within a myenteric ganglion exposed to ADP of n = 6–7 ganglia from at least 3 mice. Scale bars = 10 μM.

    Journal: Journal of Neurophysiology

    Article Title: The acute inhibition of enteric glial metabolism with fluoroacetate alters calcium signaling, hemichannel function, and the expression of key proteins

    doi: 10.1152/jn.00507.2016

    Figure Lengend Snippet: Sox10-PC::G5-tdT transgenic mice are an effective model to study glial [Ca 2+ ] i responses specifically in the enteric nervous system. A : model of our experimental paradigm comparing Fluo-4 and GCaMP5G imaging in the colonic myenteric plexus. Note that Fluo-4 loads both enteric neurons and glia, whereas GCaMP5G expression is isolated to enteric glia in our genetic model. B : representative epifluorescence images showing the specific expression of tdT (red, left ) in S100β-immunoreactive enteric glia (grayscale, middle ) in the colonic myenteric plexus of Sox10-PC::G5-tdT mice (overlay, right ). C : representative still images from a Ca 2+ imaging experiment showing GCaMP5G fluorescence (grayscale, middle 2 ) in enteric glia (identified by tdT fluorescence; red, left ) in a myenteric ganglion from a Sox10-PC::G5-tdT mouse. GCaMP5G fluorescence is low at rest (baseline) and increases robustly when glial [Ca 2+ ] i responses are stimulated by ADP (100 μM; peak). Note that [Ca 2+ ] i responses are limited to tdT-positive glial cells (overlay, right ). D : representative traces of [Ca 2+ ] i levels in enteric glia (black traces) within a myenteric ganglion (averaged response of all glia within ganglion, overlaid in green) from Sox10-PC::G5-tdT mice exposed to an ADP dose-response curve (10 µM, 100 µM, and 1 mM, subsequently). ΔF/F, change in fluorescence. E : representative traces comparing the mean [Ca 2+ ] i responses of myenteric glia with ADP recorded using GCaMP5G fluorescence of n = 20 glia from a single myenteric ganglion from Sox10-PC :: G5-tdT mice (magenta) or traditional Fluo-4 loading of n = 17 glia from a single myenteric ganglion from background control animals (green). F : GCaMP5G fluorescence reports an average peak [Ca 2+ ] i response that is comparable with those reported by Fluo-4. Peak responses are the average of all glia within a myenteric ganglion exposed to ADP of n = 6–7 ganglia from at least 3 mice. Scale bars = 10 μM.

    Article Snippet: Images were acquired through the 40× (0.75 numerical aperture, PlanFluor) objective of an upright epifluorescence microscope (Eclipse Ni; Nikon, Melville, NY) with a Retiga 2000R camera (QImaging, Surrey, BC, Canada) controlled by QCapture Pro 7.0 (QImaging).

    Techniques: Transgenic Assay, Mouse Assay, Imaging, Expressing, Isolation, Fluorescence

    The effect of fluoroacetate (FA) on hemichannel-dependent dye uptake by myenteric glia in the mouse colon. A and B : representative epifluorescence images showing ethidium bromide (EtBr) fluorescence in whole-mount preparations of the myenteric plexus from the mouse colon exposed to buffer ( A ) or ADP ( B ). Glial cells within myenteric ganglia (outlined by dashed lines) normally display a low amount of dye uptake ( A ) that increases robustly when stimulated with ADP ( B ). C : quantification of the effects of FA, the connexin-43 mimetic peptide 43Gap26, the Ca 2+ chelator EGTA, and ADP on mean glial cell EtBr fluorescence in whole-mount preparations of the mouse myenteric plexus. Scale bar ( B ) = 10 μm and applies to A and B . Measurements are representative of n = 225 glia to 373 glial cells from at least 3 mice. **** P

    Journal: Journal of Neurophysiology

    Article Title: The acute inhibition of enteric glial metabolism with fluoroacetate alters calcium signaling, hemichannel function, and the expression of key proteins

    doi: 10.1152/jn.00507.2016

    Figure Lengend Snippet: The effect of fluoroacetate (FA) on hemichannel-dependent dye uptake by myenteric glia in the mouse colon. A and B : representative epifluorescence images showing ethidium bromide (EtBr) fluorescence in whole-mount preparations of the myenteric plexus from the mouse colon exposed to buffer ( A ) or ADP ( B ). Glial cells within myenteric ganglia (outlined by dashed lines) normally display a low amount of dye uptake ( A ) that increases robustly when stimulated with ADP ( B ). C : quantification of the effects of FA, the connexin-43 mimetic peptide 43Gap26, the Ca 2+ chelator EGTA, and ADP on mean glial cell EtBr fluorescence in whole-mount preparations of the mouse myenteric plexus. Scale bar ( B ) = 10 μm and applies to A and B . Measurements are representative of n = 225 glia to 373 glial cells from at least 3 mice. **** P

    Article Snippet: Images were acquired through the 40× (0.75 numerical aperture, PlanFluor) objective of an upright epifluorescence microscope (Eclipse Ni; Nikon, Melville, NY) with a Retiga 2000R camera (QImaging, Surrey, BC, Canada) controlled by QCapture Pro 7.0 (QImaging).

    Techniques: Fluorescence, Mouse Assay