Journal: Cell Death & Disease

Article Title: Chromatin complexes subunit BAP18 promotes triple-negative breast cancer progression through transcriptional activation of oncogene S100A9

doi: 10.1038/s41419-022-04785-x

Figure Lengend Snippet: A Cellular lysates from 11 TNBC cell lines and human mammary epithelial cell line MCF10A were subjected to immunoblotting analysis with the indicated antibodies. B Ectopic expression of BAP18 in SUM159PT and Hs578T cells by lentiviral infections. The expression status of BAP18 in these cell lines was validated by immunoblotting. C – E SUM159PT and Hs578T cells stably expressing pCDH and Flag-BAP18 were subjected to CCK-8 assays ( C ) and colony formation assays ( D – E ). Representative images of survival colonies and corresponding quantitative results are shown in D and E , respectively. F , G SUM159PT and Hs578T cells stably expressing pCDH and Flag-BAP18 were subjected to Transwell migration and Matrigel invasion assays. Representative images of migrated and invaded cells and corresponding quantitative results are shown in F and G , respectively. * p < 0.05; *** p < 0.001.

Article Snippet: For transwell migration and invasion assays, cells at a density of 4 × 10 4 per well without FBS were seeded into upper transwell chamber (Corning Falcon, #353097) for cell migration assays and upper Matrigel invasion chamber (Corning BioCoat, #354480) for cell invasion assays.

Techniques: Western Blot, Expressing, Stable Transfection, CCK-8 Assay, Migration

Journal: Cell Death & Disease

Article Title: Chromatin complexes subunit BAP18 promotes triple-negative breast cancer progression through transcriptional activation of oncogene S100A9

doi: 10.1038/s41419-022-04785-x

Figure Lengend Snippet: A Knockdown of endogenous BAP18 in MDA-MB-231 and LM2-4175 cells by lentiviral infections with two independent shRNAs targeting BAP18 (shBAP18 #1 and #3). The expression status of BAP18 in these cell lines was validated by immunoblotting. B – D MDA-MB-231 and LM2-4175 cells stably expressing shNC and shBAP18 were subjected to CCK-8 assays ( B ) and colony formation assays ( C , D ). Representative images of survival colonies and corresponding quantitative results are shown in C and D , respectively. E – G MDA-MB-231 and LM2-4175 cells stably expressing shNC and shBAP18 were subjected to Transwell migration and Matrigel invasion assays. Representative images of migrated and invaded cells ( E ) and corresponding quantitative results ( F , G ) are shown, respectively. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: For transwell migration and invasion assays, cells at a density of 4 × 10 4 per well without FBS were seeded into upper transwell chamber (Corning Falcon, #353097) for cell migration assays and upper Matrigel invasion chamber (Corning BioCoat, #354480) for cell invasion assays.

Techniques: Knockdown, Expressing, Western Blot, Stable Transfection, CCK-8 Assay, Migration