Journal: OncoTargets and therapy

Article Title: miR-495 inhibits proliferation, migration, and invasion and induces apoptosis via inhibiting PBX3 in melanoma cells

doi: 10.2147/OTT.S152362

Figure Lengend Snippet: miR-495 inhibits melanoma cell proliferation, migration, invasion, and colony formation in vitro. ( A ) A375 and MeWo cells were transfected with miR-495 mimics, negative control (miR-NC), negative control inhibitor (NC inhibitor), or miR-495 inhibitor for 24 hours, then the expression of miR-495 was quantified by qRT-PCR. ( B ) miR-495 mimics, miR-NC, NC inhibitor or miR-495 inhibitor-transfected A375, and MeWo cells were subjected to MTT assay. ( C ) miR-495 mimics, miR-NC, NC inhibitor or miR-495 inhibitor-transfected A375, and MeWo cells were subjected to wound healing assay. ( D ) miR-495 mimics, miR-NC, NC inhibitor or miR-495 inhibitor-transfected A375, and MeWo cells were subjected to transwell assay. ( E ) miR-495 mimics, miR-NC, NC inhibitor or miR-495 inhibitor-transfected A375, and MeWo cells were subjected to colony formation assay. Data are presented as mean ± SD (n=3). All experiments were performed three times, and representative images are presented. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: About 1×10 5 cells were plated on the upper Transwell chambers (Costar; Corning Incorporated, Corning, NY, USA) with Matrigel (BD Bioscience, San Jose, CA, USA).

Techniques: Migration, In Vitro, Transfection, Negative Control, Expressing, Quantitative RT-PCR, MTT Assay, Wound Healing Assay, Transwell Assay, Colony Assay

Journal: OncoTargets and therapy

Article Title: miR-495 inhibits proliferation, migration, and invasion and induces apoptosis via inhibiting PBX3 in melanoma cells

doi: 10.2147/OTT.S152362

Figure Lengend Snippet: Silencing of pre-B-cell leukemia transcription factor 3 (PBX3)-inhibited cell proliferation, migration, and invasion in melanoma cells. ( A ) A375 and MeWo cells were transfected with negative control siRNA (si-NC) or siRNA against PBX3 (si-PBX3) for 24 hours, the mRNA expression levels of PBX3 in both the cells were examined by RT-qPCR and ( B ) the protein levels of PBX3 in both the cells were examined by Western blot. ( C ) A375 and MeWo cells were transfected with si-NC or si-PBX3 for indicated time, and cell proliferation was analyzed by MTT assay. ( D ) A375 and MeWo cells were transfected with si-NC or by transwell assay. ( E ) Cell migration was analyzed by si-PBX3 for 24 hours, and cell invasion was analyzed by wound healing assay. Data are presented as mean ± SD (n=3). All experiments were performed three times, and representative images are presented. * P <0.05, ** P <0.01, *** P <0.001. Abbreviation: NC, negative control.

Article Snippet: About 1×10 5 cells were plated on the upper Transwell chambers (Costar; Corning Incorporated, Corning, NY, USA) with Matrigel (BD Bioscience, San Jose, CA, USA).

Techniques: Migration, Transfection, Negative Control, Expressing, Quantitative RT-PCR, Western Blot, MTT Assay, Transwell Assay, Wound Healing Assay

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Overexpressed MPS-1 contributes to endometrioma development through the NF-κB signaling pathway

doi: 10.1186/s12958-021-00796-z

Figure Lengend Snippet: MPS-1 regulates proliferation, migration, invasion, apoptosis and cell cycle of EcESCs. A Transfection efficiency of MPS-1 siRNA was detected by qRT-PCR. B A representative western blotting image of MPS-1 after EcESCs were transfected with MPS-1 siRNA for 72 h (n = 2). C Based on the results of western blotting, knockdown efficiency of MPS-1 siRNA was analyzed by Image J software. D The effects of MPS-1 siRNA on proliferation were evaluated by CCK-8 assay. E , G The representative images of EcESCs apoptosis and cell cycle were depicted by flow cytometry after MPS-1 siRNA transfection for 72 h. F , H The apoptosis analysis and cell cycle analysis were based on the results of flow cytometry. I , K Transwell assays were used to determine the effects of MPS-1 siRNA on migration and invasion. The scale bar is 50 μm. J , L Cell count analysis of Transwell migration and invasion. NC, si-MPS-1 EcESCs transfected with negative control siRNA or MPS-1 siRNA (n = 4). Data are presented as mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

Article Snippet: After 48 h of transfection, 5 × 10 4 EcESCs in 200 μL DMEM/F12 were seeded onto the upper transwell chamber (BD Falcon, USA).

Techniques: Migration, Transfection, Quantitative RT-PCR, Western Blot, Knockdown, Software, CCK-8 Assay, Flow Cytometry, Cell Cycle Assay, Cell Counting, Negative Control