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  • 99
    Agilent technologies uplc
    Uplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uplc/product/Agilent technologies
    Average 99 stars, based on 160 article reviews
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    uplc - by Bioz Stars, 2020-03
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    96
    Millipore uplc
    Uplc, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 32 article reviews
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    99
    Waters Corporation uplc
    <t>UPLC-Q-TOF-MS</t> total ion chromatogram (TIC) of purified <t>PPP</t> and the ion chromatograms of representative peaks. ( A ) Total ion chromatographic profiling of purified PPP; ( B ) MS/MS spectrum of peak 1; ( C ) MS/MS spectrum of peak 8; ( D ) MS/MS spectrum of peak 9; ( E ) MS/MS spectrum of peak 13; ( F ) MS/MS spectrum of peak 22. Mass spectral data positive mode of phenolics.
    Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 2200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uplc/product/Waters Corporation
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    99
    Shimadzu Corporation uplc
    Kinetic analysis of the reaction of HOSCN with <t>Fmoc–SeMet</t> and isolated purified GPx by competition kinetics ( A ) Linear analysis (see the text and [ 23 ] for derivation of equation) for competitive reaction between Fmoc–SeMet (5 μM) and SeMet (0–50 μM) with HOSCN. Results are means±S.E.M. ( B ) <t>UPLC</t> (with fluorescence detection) was used to monitor the formation of the selenoxide of Fmoc–SeMet (retention time, 1.65 min) following reaction of Fmoc–SeMet (5 μM) with HOSCN (1 μM) in 0.1 M phosphate buffer (pH 7.4) at 22°C. Increasing concentrations of GPx (up to 3.9 μM; tetramer concentrations for each trace shown) were included in the reaction mixture to assess the relative rate constants for GPx compared with Fmoc–SeMet. a.u., absorbance units. ( C ) Linear analysis (as above) for the competitive kinetic data for GPx with HOSCN. Individual data points for each replicate are shown. Y, yield.
    Uplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uplc/product/Shimadzu Corporation
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    99
    Thermo Fisher uplc
    Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or <t>UPLC-ToF–MS</t> high resolution mass
    Uplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Dikma Technologies uplc
    Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or <t>UPLC-ToF–MS</t> high resolution mass
    Uplc, supplied by Dikma Technologies, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Fisher Scientific uplc
    Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or <t>UPLC-ToF–MS</t> high resolution mass
    Uplc, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Shimadzu Corporation uplc qqq ms the uplc system
    Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or <t>UPLC-ToF–MS</t> high resolution mass
    Uplc Qqq Ms The Uplc System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 87/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uplc qqq ms the uplc system/product/Shimadzu Corporation
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    99
    Agilent technologies 1290 uplc
    Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or <t>UPLC-ToF–MS</t> high resolution mass
    1290 Uplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation acquity uplc
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Acquity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Waters Corporation aquitiy uplc
    <t>RP-UPLC-DAD</t> profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a <t>ACQUITY</t> Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.
    Aquitiy Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation nanoacquity uplc
    Schematic outline for MS and LC-MS/MS experiments. (A) Direct infusion experiment using ESI-QTOF Micro MS. (B) NanoLC-MS/MS using <t>nanoACQUITY</t> <t>UPLC</t> coupled with QTOF Ultima API MS.
    Nanoacquity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nanoaquity uplc
    Schematic outline for MS and LC-MS/MS experiments. (A) Direct infusion experiment using ESI-QTOF Micro MS. (B) NanoLC-MS/MS using <t>nanoACQUITY</t> <t>UPLC</t> coupled with QTOF Ultima API MS.
    Nanoaquity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation rp uplc
    Figure 2. Electrophoretic mobility and <t>RP-UPLC</t> analysis of tHA-BC. <t>Coomassie-stained</t> SDS-PAGE of ( A ) HAC1 and ( B ) tHA-BC. Molecular weight markers (lane 1), HAC1 monomer (lanes 2–4) or tHA-BC (lanes 5–8) at various loads. tHA-BC
    Rp Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation uplc analysis
    <t>UPLC</t> chromatograms, and UV spectra of GA, MG, and <t>PGG</t> as the reference standards ( A1 and A2 ) and as principles in MSKE ( B1 and B2 ).
    Uplc Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation uplc column
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Biosolve uplc grade
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc Grade, supplied by Biosolve, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation uplc ms
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies uplc pump
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc Pump, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation uplc qtofms
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc Qtofms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Shimadzu Corporation uplc system
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Waters Corporation uplc tqd
    Characterization of the main anthocyanins in Brunfelsia flower, using <t>UPLC-QTOF-MS/MS.</t> (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.
    Uplc Tqd, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Waters Corporation uplc tqs
    Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) value of quantitative data obtained by <t>UPLC-TQS.</t> Only the first leaves were used.
    Uplc Tqs, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation acquitytm uplc
    Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) value of quantitative data obtained by <t>UPLC-TQS.</t> Only the first leaves were used.
    Acquitytm Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation aquity uplc
    Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) value of quantitative data obtained by <t>UPLC-TQS.</t> Only the first leaves were used.
    Aquity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Shimadzu Corporation nextera uplc
    Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) value of quantitative data obtained by <t>UPLC-TQS.</t> Only the first leaves were used.
    Nextera Uplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation uplc system
    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. <t>Glycoforms</t> were separated by <t>UPLC-FLR</t> system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show
    Uplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Waters Corporation uplc tofms
    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. <t>Glycoforms</t> were separated by <t>UPLC-FLR</t> system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show
    Uplc Tofms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Waters Corporation masstrak uplc
    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. <t>Glycoforms</t> were separated by <t>UPLC-FLR</t> system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show
    Masstrak Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Waters Corporation nanoaccuity uplc
    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. <t>Glycoforms</t> were separated by <t>UPLC-FLR</t> system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show
    Nanoaccuity Uplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Shimadzu Corporation nexera uplc
    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. <t>Glycoforms</t> were separated by <t>UPLC-FLR</t> system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show
    Nexera Uplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 87/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co uplc analysis
    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. <t>Glycoforms</t> were separated by <t>UPLC-FLR</t> system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show
    Uplc Analysis, supplied by Merck & Co, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    UPLC-Q-TOF-MS total ion chromatogram (TIC) of purified PPP and the ion chromatograms of representative peaks. ( A ) Total ion chromatographic profiling of purified PPP; ( B ) MS/MS spectrum of peak 1; ( C ) MS/MS spectrum of peak 8; ( D ) MS/MS spectrum of peak 9; ( E ) MS/MS spectrum of peak 13; ( F ) MS/MS spectrum of peak 22. Mass spectral data positive mode of phenolics.

    Journal: Molecules

    Article Title: Optimization of Purification, Identification and Evaluation of the inVitro Antitumor Activity of Polyphenols from Pinus Koraiensis Pinecones

    doi: 10.3390/molecules200610450

    Figure Lengend Snippet: UPLC-Q-TOF-MS total ion chromatogram (TIC) of purified PPP and the ion chromatograms of representative peaks. ( A ) Total ion chromatographic profiling of purified PPP; ( B ) MS/MS spectrum of peak 1; ( C ) MS/MS spectrum of peak 8; ( D ) MS/MS spectrum of peak 9; ( E ) MS/MS spectrum of peak 13; ( F ) MS/MS spectrum of peak 22. Mass spectral data positive mode of phenolics.

    Article Snippet: Identification of Purified PPP by UPLC-Q-TOF-MS The purified PPP was analyzed using a UPLC (ACQUITY™, Waters Technologies, Milford, MA, USA) equipped with a HSS T3 column (2.1 × 100 mm, 1.8 μm, waters).

    Techniques: Mass Spectrometry, Purification

    Typical UPLC chromatograms of plant samples using DAD at 203 nm.

    Journal: BMC Biochemistry

    Article Title: Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS

    doi: 10.1186/1471-2091-15-19

    Figure Lengend Snippet: Typical UPLC chromatograms of plant samples using DAD at 203 nm.

    Article Snippet: UPLC–DAD–MS instrumentation and conditions A Waters Acquity™ ultra performance liquid chromatography (UPLC) system (Waters Corp., Milford, USA) with photodiode array detection (PAD), was hyphenated to a Bruker MicrOTOFQ system by an electrospray ionization (ESI) interface (Bruker Daltonics, Bremen, Germany) for chromatographic and mass spectrometric (MS) analysis.

    Techniques:

    UPLC chromatogram of four marker compounds in BSE. (A) UPLC chromatogram of commercial standard compounds. (B) UPLC chromatogram of four marker compounds in BSE. The chromatograms were obtained at 277 nm (a, b, and c) and 280 nm (d). Baicalin (a), baicalein (b), wogonin (c), and berberine (d). BSE: Bojesodok-eum.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Bojesodok-eum, a Herbal Prescription, Ameliorates Acute Inflammation in Association with the Inhibition of NF- κB-Mediated Nitric Oxide and ProInflammatory Cytokine Production

    doi: 10.1155/2012/457370

    Figure Lengend Snippet: UPLC chromatogram of four marker compounds in BSE. (A) UPLC chromatogram of commercial standard compounds. (B) UPLC chromatogram of four marker compounds in BSE. The chromatograms were obtained at 277 nm (a, b, and c) and 280 nm (d). Baicalin (a), baicalein (b), wogonin (c), and berberine (d). BSE: Bojesodok-eum.

    Article Snippet: Profiling the Chemical Contents of BSE by UPLC The UPLC (ultraperformance liquid chromatography) system (Waters, USA), which was equipped with a pump Waters ACQUITY ultraperformance LC system (USA) and a Waters ACQUITY photodiode array detector (PDA), was used for analysis.

    Techniques: Marker

    Kinetic analysis of the reaction of HOSCN with Fmoc–SeMet and isolated purified GPx by competition kinetics ( A ) Linear analysis (see the text and [ 23 ] for derivation of equation) for competitive reaction between Fmoc–SeMet (5 μM) and SeMet (0–50 μM) with HOSCN. Results are means±S.E.M. ( B ) UPLC (with fluorescence detection) was used to monitor the formation of the selenoxide of Fmoc–SeMet (retention time, 1.65 min) following reaction of Fmoc–SeMet (5 μM) with HOSCN (1 μM) in 0.1 M phosphate buffer (pH 7.4) at 22°C. Increasing concentrations of GPx (up to 3.9 μM; tetramer concentrations for each trace shown) were included in the reaction mixture to assess the relative rate constants for GPx compared with Fmoc–SeMet. a.u., absorbance units. ( C ) Linear analysis (as above) for the competitive kinetic data for GPx with HOSCN. Individual data points for each replicate are shown. Y, yield.

    Journal: Biochemical Journal

    Article Title: Selenium-containing amino acids are targets for myeloperoxidase-derived hypothiocyanous acid: determination of absolute rate constants and implications for biological damage

    doi: 10.1042/BJ20101762

    Figure Lengend Snippet: Kinetic analysis of the reaction of HOSCN with Fmoc–SeMet and isolated purified GPx by competition kinetics ( A ) Linear analysis (see the text and [ 23 ] for derivation of equation) for competitive reaction between Fmoc–SeMet (5 μM) and SeMet (0–50 μM) with HOSCN. Results are means±S.E.M. ( B ) UPLC (with fluorescence detection) was used to monitor the formation of the selenoxide of Fmoc–SeMet (retention time, 1.65 min) following reaction of Fmoc–SeMet (5 μM) with HOSCN (1 μM) in 0.1 M phosphate buffer (pH 7.4) at 22°C. Increasing concentrations of GPx (up to 3.9 μM; tetramer concentrations for each trace shown) were included in the reaction mixture to assess the relative rate constants for GPx compared with Fmoc–SeMet. a.u., absorbance units. ( C ) Linear analysis (as above) for the competitive kinetic data for GPx with HOSCN. Individual data points for each replicate are shown. Y, yield.

    Article Snippet: Oxidation of Fmoc–SeMet to the corresponding selenoxide [Fmoc–MetSeO (methionine selenoxide)] was monitored by UPLC (ultra-performance liquid chromatography) (Shimadzu Nexera system) with fluorescence detection (RF-20Axs detector; λex 265 nm; λem 310 nm).

    Techniques: Isolation, Purification, Fluorescence, Relative Rate

    Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or UPLC-ToF–MS high resolution mass

    Journal: Metabolomics

    Article Title: Metabolomics-guided analysis of isocoumarin production by Streptomyces species MBT76 and biotransformation of flavonoids and phenylpropanoids

    doi: 10.1007/s11306-016-1025-6

    Figure Lengend Snippet: Biotransformation of genistein by S treptomyces sp. MBT76. Products were identified on the basis of NMR and/or UPLC-ToF–MS high resolution mass

    Article Snippet: UPLC-ToF–MS analysis UPLC-ToF–MS analyses were performed on an UPLC system (Ultimate 3000, ThermoScientific, Germany) coupled to an ESI-llQ-ToF spectrometer (micrOToF-QII, Bruker Daltonics, Germany) in the positive mode.

    Techniques: Nuclear Magnetic Resonance, Mass Spectrometry

    LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

    Journal: Nature Methods

    Article Title: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

    doi: 10.1038/s41592-019-0457-0

    Figure Lengend Snippet: LC-MS of protein standard mixture prepared following Protocol 5a and separated on a Dionex UPLC with a Thermo Orbitrap Elite system using PLRP-S stationary phase. Final concentrations of each protein loaded onto the column were 0.14 pmol ubiquitin, 0.49 pmol trypsinogen, 1.09 pmol myoglobin and 0.64 pmol carbonic anhydrase (top). Summary of S/N values calculated for each protein on all instrumentation platforms using the given SOP (bottom) including Dionex Ultimate 3000–Thermo Orbitrap Elite, Waters Acquity–Xevo G2-S QTOF, Waters nanoAcquity–Bruker Impact II QTOF, Waters nanoAcquity–Bruker SolariX FT-ICR, Dionex Ultimate 3000–Thermo Fusion Lumos, and Dionex Ultimate 3000–Thermo QE-HF. As described, S/N calculations differ per manufacturer and do not reflect absolute performance.

    Article Snippet: Samples were prepared following the given SOP and separated on a Dionex UPLC coupled to (a.) a Thermo Orbitrap Elite (monolithic stationary phase, (b.) a Thermo Orbitrap Fusion Lumos (PLRP-S stationary phase), (c.) a Thermo Orbitrap QE-HF (PLRP-S stationary phase).

    Techniques: Liquid Chromatography with Mass Spectroscopy

    RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.

    Journal: Mediators of Inflammation

    Article Title: Protective Effect of Baccharis trimera Extract on Acute Hepatic Injury in a Model of Inflammation Induced by Acetaminophen

    doi: 10.1155/2014/196598

    Figure Lengend Snippet: RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera . Conditions: CHS130 100 RP-18 column (1.7 μ m, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.

    Article Snippet: The ESI-MS/MS analyses were additionally performed in an UPLC Acquity (Waters) with helium as the collision gas, and the collision energy was set at 30 eV.

    Techniques: Mass Spectrometry, Flow Cytometry

    Schematic outline for MS and LC-MS/MS experiments. (A) Direct infusion experiment using ESI-QTOF Micro MS. (B) NanoLC-MS/MS using nanoACQUITY UPLC coupled with QTOF Ultima API MS.

    Journal: Electrophoresis

    Article Title: Mass spectrometry-based proteomics of oxidative stress: Identification of 4-hydroxy-2-nonenal (HNE) adducts of amino acids using lysozyme and bovine serum albumin as model proteins

    doi: 10.1002/elps.201600134

    Figure Lengend Snippet: Schematic outline for MS and LC-MS/MS experiments. (A) Direct infusion experiment using ESI-QTOF Micro MS. (B) NanoLC-MS/MS using nanoACQUITY UPLC coupled with QTOF Ultima API MS.

    Article Snippet: HNE-treated lysozyme and BSA, as well as their untreated (control) counterparts, were subjected to SDS-PAGE, then stained with Coomassie Blue; the gel bands were excised, subjected to in-gel trypsin digestion, and the resultant peptides extracted and purified (C18 Ziptip™; Millipore, Billerica, MA), following by nanoLC-MS/MS analysis using a nanoACQUITY® UPLC coupled with a QTOF Ultima API mass spectrometer (Waters, Milford, MA), as previously described [ ]. shows a schematic outline of the direct infusion experiment ( ) and the nanoLC-MS/MS experiment , respectively.

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Figure 2. Electrophoretic mobility and RP-UPLC analysis of tHA-BC. Coomassie-stained SDS-PAGE of ( A ) HAC1 and ( B ) tHA-BC. Molecular weight markers (lane 1), HAC1 monomer (lanes 2–4) or tHA-BC (lanes 5–8) at various loads. tHA-BC

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge

    doi: 10.4161/hv.23234

    Figure Lengend Snippet: Figure 2. Electrophoretic mobility and RP-UPLC analysis of tHA-BC. Coomassie-stained SDS-PAGE of ( A ) HAC1 and ( B ) tHA-BC. Molecular weight markers (lane 1), HAC1 monomer (lanes 2–4) or tHA-BC (lanes 5–8) at various loads. tHA-BC

    Article Snippet: Purity of the purified tHA-BC protein was determined by separation on a 10% SDS-PAGE gel followed by staining with Coomassie Gel Code blue (Pierce, Rochford, IL), as well as by RP-UPLC using a Waters Acquity system (Waters, Milford, MA) with a BEH C4 column and water/acetonitrile (both containing 0.1% trifluoroacetic acid) mobile phases.

    Techniques: Staining, SDS Page, Molecular Weight

    UPLC chromatograms, and UV spectra of GA, MG, and PGG as the reference standards ( A1 and A2 ) and as principles in MSKE ( B1 and B2 ).

    Journal: Molecules

    Article Title: Factors Influencing Oral Bioavailability of Thai Mango Seed Kernel Extract and Its Key Phenolic Principles

    doi: 10.3390/molecules201219759

    Figure Lengend Snippet: UPLC chromatograms, and UV spectra of GA, MG, and PGG as the reference standards ( A1 and A2 ) and as principles in MSKE ( B1 and B2 ).

    Article Snippet: UPLC Analysis of GA, MG and PGG A UPLC was performed using an AcquityTM system (Waters, Milford, MA, USA) equipped with a diode-arrayed detector and Empower software.

    Techniques:

    Characterization of the main anthocyanins in Brunfelsia flower, using UPLC-QTOF-MS/MS. (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.

    Journal: Journal of Experimental Botany

    Article Title: Metabolic networking in Brunfelsia calycina petals after flower opening

    doi: 10.1093/jxb/erq008

    Figure Lengend Snippet: Characterization of the main anthocyanins in Brunfelsia flower, using UPLC-QTOF-MS/MS. (A) A UV chromatogram at 530 nm of the different anthocyanins in Brunfelsia flower petals at D0. (B) The main anthocyanins detected in Brunfelsia flowers.

    Article Snippet: Mass spectral analyses were carried out by the ultraperformance liquid chromatography–quadrupole time of flight (UPLC-QTOF) instrument (Waters Premier QTOF, Milford, MA, USA), with the UPLC column connected on-line to a UV detector (measuring at 530 nm; Waters Acquity), and then to the MS detector equipped with an electrospray ion (ESI) source (performed in ESI-positive mode).

    Techniques: Mass Spectrometry

    Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) value of quantitative data obtained by UPLC-TQS. Only the first leaves were used.

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops

    doi: 10.1098/rspb.2015.0964

    Figure Lengend Snippet: Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) value of quantitative data obtained by UPLC-TQS. Only the first leaves were used.

    Article Snippet: Quantitative data for metabolite accumulation were obtained by UPLC-TQS (Waters, Tokyo, Japan) as described previously [ ].

    Techniques:

    Metabolite profiling of the seeds and leaves of Bd21, Bd3-1 and Chinese Spring wheat at different growth stages. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) values of quantitative data, which were obtained by UPLC-TQS. Only the first leaves were used as leaf samples.

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops

    doi: 10.1098/rspb.2015.0964

    Figure Lengend Snippet: Metabolite profiling of the seeds and leaves of Bd21, Bd3-1 and Chinese Spring wheat at different growth stages. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log 2 ) values of quantitative data, which were obtained by UPLC-TQS. Only the first leaves were used as leaf samples.

    Article Snippet: Quantitative data for metabolite accumulation were obtained by UPLC-TQS (Waters, Tokyo, Japan) as described previously [ ].

    Techniques:

    N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. Glycoforms were separated by UPLC-FLR system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show

    Journal: mAbs

    Article Title: Surrogate target cells expressing surface anti-idiotype antibody for the clinical evaluation of an internalizing CD22-specific antibody

    doi: 10.4161/19420862.2014.985519

    Figure Lengend Snippet: N-linked glycan analysis of SM06 produced from SP2/0 and CHO. Glycan release was performed by PNGase. Glycoforms were separated by UPLC-FLR system equipped with a BEH Glycan column and were verified by Q-TOF mass spectrometry. Inserted pie charts show

    Article Snippet: The labeled glycoforms (2 μL) were separated by an UPLC system equipped with a BEH Glycan column (bead size: 1.7 μm, dimensions: 2.1 mm × 150 mm; Waters Corp., Milford, MA) thermostated at 60°C.

    Techniques: Produced, Mass Spectrometry