Journal: BioMed Research International

Article Title: Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated

doi: 10.1155/2017/6972030

Figure Lengend Snippet: Representative 2D-DIGE gel image of differentially expressed proteins of fetal ovaries at day 55 and day 90 of gestation. The proteins extracted from the fetal ovaries at day 55 and day 90 of gestation samples were labelled with cy3 and cy5, respectively. An internal standard protein sample (a mixture of fetal ovaries at day 55 and day 90 of gestation samples) was labelled with the Cy2 dye. The number in the figure corresponds to the number shown in .

Article Snippet: The 2D DIGE gel images were analyzed by the Image Master 2D platinum 7.0 software (GE Healthcare Life Sciences, NJ, USA) and the protein abundance changes for spot picking detection were calculated using cy3/cy2 and cy5/cy2 differential in-gel analysis ratios.

Techniques:

Journal: Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology

Article Title: Coronary microvascular dysfunction, left ventricular remodeling, and clinical outcomes in aortic stenosis

doi: 10.1007/s12350-019-01706-y

Figure Lengend Snippet: PET myocardial blood flow, myocardial flow reserve, and echocardiographic measures of myocardial structure and function in control patients and patients with aortic sclerosis or stenosis

Article Snippet: Conventional 2D echocardiography (Siemens, Tarrytown, NY) and speckle tracking 2D echocardiography analysis (TomTec Imaging Systems, Germany) were performed offline.

Techniques: Control

Journal:

Article Title: Transcription-dependent recombination and the role of fork collision in yeast rDNA

doi: 10.1101/gad.1085403

Figure Lengend Snippet: Analysis of low-copy-rDNA strains. (A) Southern hybridization analysis of rDNA copy numbers. DNA was digested with BglII and subjected to electrophoresis followed by Southern analysis using the rDNA probe (Fig. 1). A single-copy gene, MCM2, was used as an internal control for normalization. (B) Quantitation of the intensities of the bands. NOY408-1b (wild-type strain), NOY408-1bf (fob1), TAK300 (fob1; low-copy rDNA strain), TAK301 (fob1 pol1; low-copy rDNA strain). (C) Collision between the transcription and the replication machineries analyzed by 2D gel analysis. DNA was prepared from the strains indicated, digested with BglII and SphI, and subjected to 2D agarose gel electrophoresis followed by Southern hybridization using the rDNA probe (see Fig. 1). A spot indicated by an arrowhead shows accumulation of Y-shaped DNA molecules at the RFB site (panel a). The slowdown region (SDR) is located between two arrows (panel c). The numbers in parentheses are copy numbers of rDNA in each strain. (Panel a) NOY408-1b (wild-type strain). (Panel b) NOY408-1bf (fob1). (Panel c) TAK300 (fob1; low-copy rDNA strain). (Panel d) TAK301 (fob1 pol1; low-copy rDNA strain). (Panel e) TAK301, complemented by a plasmid-borne RPA135 gene (fob1 POLI; low-copy rDNA strain).

Article Snippet: Replication fork blocking and slowdown activities were analyzed using 2D gel electrophoresis as described previously ( Brewer and Fangman 1987 ).

Techniques: Hybridization, Electrophoresis, Control, Quantitation Assay, Two-Dimensional Gel Electrophoresis, Agarose Gel Electrophoresis, Plasmid Preparation