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Advanced Biotechnologies Inc
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Autogen-Bioclear ltd
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Biotest AG
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Promega
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AMBIS TECHNOLOGIES PVT LTD
ul44 protein ![]() Ul44 Protein, supplied by AMBIS TECHNOLOGIES PVT LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/ul44/ul44+protein/pm09367818-157-5-25 Average 90 stars, based on 1 article reviews
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MultiSynTech gmbh
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ToRCH is an acronym for a group of infections that can cause congenital abnormalities and it has become an important part of prenatal care to recognize these infections in the first trimester of pregnancy. For
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Cytomegalovirus (CMV) pp52 (UL44) Recombinant
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Myc DDK tagged ORF clone of viral ORF for envelope glycoprotein C Human herpesvirus 1 codon optimized for human cell expression NP 044646
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The E.coli derived 51 kDa recombinant protein contains the CMV Pp52 (UL44) immunodominant regions, 202-434 amino acids. Recombinant CMV-Pp52 is fused to a 26 kDa GST tag
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Image Search Results
Journal: PLoS ONE
Article Title: The Chromatin Remodeling Factor SMARCB1 Forms a Complex with Human Cytomegalovirus Proteins UL114 and UL44
doi: 10.1371/journal.pone.0034119
Figure Lengend Snippet: ( A ) SMARCB1 co-localizes with UL44 in HCMV-infected fibroblast cells harvested at 24, 48, and 72 hpi. ( B ) Co-localization of SMARCB1 and other essential factors of the SWI/SNF complex; BRG-1, BAF155, BAF170, in HCMV infected fibroblast cells harvested at 48 hpi. The cells were fixed and subjected to double-staining for UL44 (mouse Mab-UL44) and SMARCB1 (rabbit Pab-SMARCB1) and SMARCB1 (rabbit Pab-SMARCB1) and either BRG-1 (mouse Mab-BRG-1), BAF155 (mouse Mab BAF155), BAF170 (mouse Mab BAF170) for immunofluorescence microscopy. Secondary antibodies were used for staining UL44, BRG-1, BAF155, BAF170 in green (anti-mouse 488) and SMARCB1 in red (anti-rabbit 594), and the cells were further visualized by confocal microscopy. Co-localization was visualized by a merge of the two microscopic determinations, and counterstaining of the nuclei was achieved by the use of DAPI.
Article Snippet: Primary antibodies used were mouse anti-GST ((3D4), sc-57753, Santa Cruz Biotechnology), goat anti-lamin A/C antibody (N-18, Santa Cruz Biotechnology), rabbit anti-histone H1 antibody (FL-219, Santa Cruz Biotechnology), mouse anti-UL57 ((anti-cytomegalovirus ICP8, CH167), ab-53493 Abcam), rabbit anti-UL114 (polyclonal ascites/serum raised against a synthetic peptide comprising amino-acids 11–28+cys of UL114),
Techniques: Infection, Double Staining, Immunofluorescence, Microscopy, Staining, Confocal Microscopy
Journal: PLoS ONE
Article Title: The Chromatin Remodeling Factor SMARCB1 Forms a Complex with Human Cytomegalovirus Proteins UL114 and UL44
doi: 10.1371/journal.pone.0034119
Figure Lengend Snippet: ( A ) The expression of UL114, SMARCB1 and UL44 in nuclear extracts (20 µg) from mock and HCMV-infected fibroblast cells was analyzed at immediate –early (12 hpi), early (24 hpi) and late (48 and 72 hpi) times of infection by western blot. The western blots were analyzed by Thyphoon scanning. GAPDH was used as a loading control. ( B ) The expression of BRG1, BAF155 and BAF 170 in nuclear extracts (40 µg) from mock and HCMV-infected fibroblast cells was analyzed at late (72 hpi) time of infection by western blot. The western blots were analyzed by Thyphoon scanning. GAPDH was used as a loading control.
Article Snippet: Primary antibodies used were mouse anti-GST ((3D4), sc-57753, Santa Cruz Biotechnology), goat anti-lamin A/C antibody (N-18, Santa Cruz Biotechnology), rabbit anti-histone H1 antibody (FL-219, Santa Cruz Biotechnology), mouse anti-UL57 ((anti-cytomegalovirus ICP8, CH167), ab-53493 Abcam), rabbit anti-UL114 (polyclonal ascites/serum raised against a synthetic peptide comprising amino-acids 11–28+cys of UL114),
Techniques: Expressing, Infection, Western Blot
Journal: PLoS ONE
Article Title: The Chromatin Remodeling Factor SMARCB1 Forms a Complex with Human Cytomegalovirus Proteins UL114 and UL44
doi: 10.1371/journal.pone.0034119
Figure Lengend Snippet: ( A ) Co-immunoprecipitation of endogenous SMARCB1 and UL44 in HCMV-infected fibroblast cells. Equal numbers of mock infected and HCMV infected fibroblast cells were lysed at 72 hpi, and the extracts were immunoprecipitated with anti-SMARCB1. Co-immunoprecipitated proteins were resolved electrophoretically and subjected to immunoblot analysis with anti-UL44 and anti-SMARCB1. Lane 1: Mock lysate immunoprecipitated with anti-SMARCB1. Lane 2: HCMV lysate immunoprecipitated with anti-SMARCB1. Lane 3: HCMV lysate immunoprecipitated with no antibody. Lanes 4 and 5, SMARCB1 and UL44 input in extracts (7 µg, 1% of the total in Lane 4 and 35 µg, 5% of the total in Lane 5). IgGHc: IgG heavy chain. ( B ) In vitro pull-down assay of GST-SMARCB1 and His 6 -UL44. Purified GST-SMARCB1 or GST incubated with purified His 6 -UL44 immobilized on magnetic His-tag Dynabeads. Samples were analyzed by SDS-PAGE and Coomassie blue staining. Lane 1: GST-SMARCB1+Dynabeads His-tag. Lane 2: His 6 -UL44+Dynabeads His-tag. Lane 3: GST-SMARCB1+His 6 -UL44+Dynabeads His-tag. Lane 4: His 6 -UL44 (2 µg, 10% of input). Lane 5: GST-SMARCB1 (2 µg, 10% of input). Lane 6: GST+His 6 -UL44+Dynabeads His-tag. Lane 7: GST (2 µg, 10% of input). Note that spontaneous cleavage of GST occurred in the GST-SMARCB1 protein sample (Lane 5). ( …… ) indicates that samples were run on the same gel.
Article Snippet: Primary antibodies used were mouse anti-GST ((3D4), sc-57753, Santa Cruz Biotechnology), goat anti-lamin A/C antibody (N-18, Santa Cruz Biotechnology), rabbit anti-histone H1 antibody (FL-219, Santa Cruz Biotechnology), mouse anti-UL57 ((anti-cytomegalovirus ICP8, CH167), ab-53493 Abcam), rabbit anti-UL114 (polyclonal ascites/serum raised against a synthetic peptide comprising amino-acids 11–28+cys of UL114),
Techniques: Immunoprecipitation, Infection, Western Blot, In Vitro, Pull Down Assay, Purification, Incubation, SDS Page, Staining