Journal: eLife

Article Title: Cellular tolerance at the µ-opioid receptor is phosphorylation dependent

doi: 10.7554/eLife.34989

Figure Lengend Snippet: ( A ) from a MOR knockout animal where the alpha-2-adrenoceptor agonist, UK14304 (3 µM) and OFQ both activate potassium currents whereas ME (30 µM) had no effect. ( B ) from a neuron where the wild type MOR (exWT) was expressed in the MOR knockout animal. In this recording ME (1 µM), morphine (10 µM) as well as UK14304 (3 µM) all caused outward currents.

Article Snippet: Naloxone was purchased from Abcam (Cambridge, MA), MK-801, from Hello Bio (Princeton, NJ), UK14304 tartrate and idazoxan (Ida) from Tocris (Bio-Techne Corp. Minneapolis, MN).

Techniques: Knock-Out

Journal: eLife

Article Title: Cellular tolerance at the µ-opioid receptor is phosphorylation dependent

doi: 10.7554/eLife.34989

Figure Lengend Snippet: Examples of recording with intracellular electrodes from three neurons. Application of ME (30 µM) had no effect on the firing rate however both UK14304 (3 µM, A ) and OFQ (1 µM, B ) inhibited firing and caused a hyperpolarization of the membrane potential. ( C ) application of muscarine (10 µM) increased the firing rate of the LC neuron.

Article Snippet: Naloxone was purchased from Abcam (Cambridge, MA), MK-801, from Hello Bio (Princeton, NJ), UK14304 tartrate and idazoxan (Ida) from Tocris (Bio-Techne Corp. Minneapolis, MN).

Techniques: Membrane