Journal: Cell reports

Article Title: TDP-43 chronic deficiency leads to dysregulation of transposable elements and gene expression by affecting R-loop and 5hmC crosstalk

doi: 10.1016/j.celrep.2023.113662

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fragmented gDNA was treated with 25 U of β-glucosyltransferase (β-GT, NEB, #M0357L) in the presence of 100 μM UDP-6-N 3 -Glu (Jena Bioscience, #CLK-076) to selectively label 5hmC into N 3 -5-gmC.

Techniques:

Journal: Archives of biochemistry and biophysics

Article Title: UGDH promotes 5-fluorouracil resistance in colorectal cancer via the ROS-activated PI3K/AKT-EEF1A2-PRDX1 pathway.

doi: 10.1016/j.abb.2025.110445

Figure Lengend Snippet: Fig. 7. ROS activate the PI3K/AKT pathway, regulating the UGDH-EEF1A2-PRDX1 axis to promote chemoresistance. (A) KEGG pathway enrichment of DEGs in UGDH-knockdown SW620 cells (n = 3 replicates). (B) Western blot of PI3K/AKT pathway proteins in HCT8 cells ± LY294002 (10 μM, 48 h, n = 3). (C) PI3K/AKT phosphorylation in HCT8 control and shUGDH cells ± 5-FU (n = 3). (D) UGDH and PI3K/AKT activation in HCT8 cells treated with H2O2 (0–200 μM, 24 h, n = 3). (E) Time-dependent UGDH activation in HCT8 cells treated with 15 μM 5-FU (0–96 h). (n = 3). (F) Western blot of PI3K/AKT pathway proteins in HCT8 cells ± LY294002 (10 μM, n = 3)/± NAC (750 μМ, n = 3). Data are mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: The primary antibodies used were against UGDH (Proteintech), EEF1A2 (Proteintech), PRDX1 (Proteintech), PI3K (CST), phospho-PI3K (CST), Akt (CST), phospho-AKT (Ser473) (CST), FLAG (Proteintech), β-ACTIN (Proteintech), GAPDH (Beyotime), cleaved caspase 3 (CST), and cleaved PARP (CST).

Techniques: Knockdown, Western Blot, Phospho-proteomics, Control, Activation Assay