Journal:

Article Title: Gene Expression in Asthmatic Airway Smooth Muscle

doi: 10.1513/pats.200705-059VS

Figure Lengend Snippet: GENE EXPRESSION STUDIES USING CULTURED AIRWAY SMOOTH MUSCLE CELLS

Article Snippet: More recently, microarrays have been applied to study asthma therapy by investigating the effects of β-agonists on gene expression in cultured airway smooth muscle cells ( 29 ). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Author ( Reference No. ) Source of Cells Exposures Method of Gene Expression Profiling Findings Lee and coworkers ( 28 ) Commercially available human airway smooth muscle cells IL-13 (100 ng/ml) Affymetrix Genechip HuGeneFL arrays coding for ∼6,500 human genes 204 Genes induced two-fold, including smooth muscle MHC, phospholipase A2, diacyglycerol kinase δ, and IL-13Rα1 Syed and coworkers ( 30 ) 3 Lung transplant donors IL-13 (10–100 ng/ml) Research Genetics and Incyte Genomics cDNA microarrays coding for 8,159 genes A large number of genes induced 1.5-fold or by FDR analyses (total no. not given), including VCAM1, IL-13Rα2, Tenascin C, and histamine receptor H1 IL-13R130Q * Jarai and coworkers ( 27 ) 2 Patients undergoing lung resection IL-13 † Affymetrix HG-U95Av2 microarrays coding for ∼12,500 genes H1 receptor and tenascin were highly induced by IL-13 TGF-β † IL-1β † McGraw and coworkers ( 29 ) Wild-type and transgenic mice overexpressing β 2 -adrenergic receptor None University of Cincinnati Genomic and Microarray Laboratory array coding for 8,734 cDNAs 319 Genes induced 164 Repressed (including phospholamban) Open in a separate window Definition of abbreviations: FDR = false discovery rate; IL-13Rα1 = IL-13 receptor α1; MHC = myosin heavy chain; RT-PCR = reverse transcription–polymerase chain reaction; TGF = transforming growth factor; VCAM = vascular cell adhesion molecule.

Techniques: Expressing, Cell Culture, Transgenic Assay, Microarray