Journal: Cancer research
Article Title: Melanoma Proteoglycan Modifies Gene Expression to Stimulate Tumor Cell Motility, Growth and Epithelial to Mesenchymal Transition
Figure Lengend Snippet: Erk 1,2 mediates enhanced c-Met expression through MITF (a) WM1341D and WM1552C/MCSP transfected cells were incubated with 2.4 μM U0126 or control compound U0124 overnight in serum free medium. Lysates were evaluated by western blot using the indicated antibodies. (b) WM1552C/MCSP cells were transfected with MITF specific or control siRNA for 48 hours and assayed for expression of C-Met, MITF, and pErk 1, 2 by western blot. (c) WM1552C/MCSP cells were transfected with c-Met specific or control siRNA for 48 hours and assayed for expression of C-Met, MITF and pErk 1,2 and MCSP by western blot.
Article Snippet: Other antibodies and reagents were purchased from the indicated companies: Anti-tubulin from Oncogene Research Products (Pasadena, CA); anti-phospho p44/42 MAPK (pErk 1,2), anti-p44/42 MAPK (Erk1,2), anti-c-Met and phospho c-Met (pc-Met), Mek inhibitor U0126 and control compound U0124 from Cell Signaling Technology, Inc (Boston, MA); anti-GST from Abcam, Inc (Boston, MA); anti-E-Cadherin from BD Transduction Labs (Lexington, KY); anti-MITF from Santa Cruz Biotech.
Techniques: Expressing, Transfection, Incubation, Western Blot