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Image Search Results
Journal: bioRxiv
Article Title: An implantable biohybrid nerve model towards synaptic deep brain stimulation
doi: 10.1101/2024.05.31.596665
Figure Lengend Snippet: a: The implantable biohybrid nerve model with a PDMS based nerve channel. b: Magnification of the biohybrid axon guidance structure that is implanted on the cortex. The flexible PDMS nerve channel can be implanted into the brain to target the visual thalamus. c: Biohybrid implant in which the PDMS channel has been replaced with a GelSH-GelNB hydrogel conduit. d: Schematic of the biohybrid implant. The microfluidic axon guidance structure is aligned on top of the Pt-based microelectrode array and seeded with neural spheroids. The implantable nerve channel is made of PDMS with an axon outlet at the end or consists of a GelSH-GelNB based hydrogel conduit vertically fabricated on top of the axon outlet. Neural spheroids are seeded into each of the 16 seeding wells to grow axons towards the nerve channel. e: The biohybrid implant is flipped around and implanted onto the cortical surface of a craniotomized mouse brain. The PDMS or hydrogel nerve channel is directed towards the lateral geniculate nucleus (LGN). f: The implant is fixed onto the mouse head using a glass window and dental cement. A headbar enables subsequent mounting on a 2-Photon microscope for imaging. During stimulation experiments the implant is connected through an omnetics connector.
Article Snippet: Axon growth in the biohybrid implant was assessed in vivo using a
Techniques: Microelectrode Array, Microscopy, Imaging