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two-color microarray platform  (Asuragen Inc)
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Asuragen Inc two-color microarray platform
Three groups of three mice received binocular HOKS for 24 h in the P→A direction with respect to the right eye. Total RNA was extracted from the left and right flocculi of each mouse and combined for each group. The RNA from each group was analyzed on a miRNA <t>microarray,</t> and subsequently combined for analysis. A. Each data point in the Volcano plot represents four variables: 1) The fold change for each miRNA is represented on abscissa (right floc./left floc), 2) Statistical probability transcription difference (p-value) of a miRNA between the left and right flocculi is represented on left ordinate, 3) Average copy number (color coded) is represented on right ordinate and 4) Frequency of occurrence a miRNA relative to all other sampled miRNAs is represented as data point diameter. B. Twelve miRNAs were screened using three criteria: 1) A fold change >2, 2) A p-value <0.005, and 3) An average copy number >256. Error bars indicate standard error of the mean. Histogram color indicates copy number according to the right ordinate in A.
Two Color Microarray Platform, supplied by Asuragen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Three groups of three mice received binocular HOKS for 24 h in the P→A direction with respect to the right eye. Total RNA was extracted from the left and right flocculi of each mouse and combined for each group. The RNA from each group was analyzed on a miRNA microarray, and subsequently combined for analysis. A. Each data point in the Volcano plot represents four variables: 1) The fold change for each miRNA is represented on abscissa (right floc./left floc), 2) Statistical probability transcription difference (p-value) of a miRNA between the left and right flocculi is represented on left ordinate, 3) Average copy number (color coded) is represented on right ordinate and 4) Frequency of occurrence a miRNA relative to all other sampled miRNAs is represented as data point diameter. B. Twelve miRNAs were screened using three criteria: 1) A fold change >2, 2) A p-value <0.005, and 3) An average copy number >256. Error bars indicate standard error of the mean. Histogram color indicates copy number according to the right ordinate in A.

Journal:

Article Title: CLIMBING FIBERS INDUCE microRNA TRANSCRIPTION IN CEREBELLAR PURKINJE CELLS

doi: 10.1016/j.neuroscience.2010.09.039

Figure Lengend Snippet: Three groups of three mice received binocular HOKS for 24 h in the P→A direction with respect to the right eye. Total RNA was extracted from the left and right flocculi of each mouse and combined for each group. The RNA from each group was analyzed on a miRNA microarray, and subsequently combined for analysis. A. Each data point in the Volcano plot represents four variables: 1) The fold change for each miRNA is represented on abscissa (right floc./left floc), 2) Statistical probability transcription difference (p-value) of a miRNA between the left and right flocculi is represented on left ordinate, 3) Average copy number (color coded) is represented on right ordinate and 4) Frequency of occurrence a miRNA relative to all other sampled miRNAs is represented as data point diameter. B. Twelve miRNAs were screened using three criteria: 1) A fold change >2, 2) A p-value <0.005, and 3) An average copy number >256. Error bars indicate standard error of the mean. Histogram color indicates copy number according to the right ordinate in A.

Article Snippet: miRNA expression profiling of mouse flocculus was performed using a two-color microarray platform (Asuragen Technical Services, Austin, TX).

Techniques: Microarray