Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: The sequence of TW1 antibody and the identification of the variable regions CDR1, CDR2, and CDR3 of the heavy chain and CDR1, CDR2, and CDR3 of the light chain.

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Sequencing

Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: Epitope mapping of TW1. The epitope mapping was performed with dot blot that shows the immunoreactivity of TW1 antibody with human PrP sequences: PrP 90-108; PrP94-123 and PrP23-231. Other PrP sequences were not recognized by the TW1 antibody.

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Dot Blot

Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: Passive immunotherapy with the TW1 antibody improves cognitive tasks in the hTau/PS1 Alzheimer’s disease (AD) mice model. Panel (A) shows working memory improvement using a short-term memory test (Novel Object Recognition Test). The bars depict the percentage of the amount of time spent with the novel or old object. Mice treated with TW1 spent more time with the novel object compared to the old object (two-tailed t -test, ** p = 0.0019). Vehicle treated mice showed no significant difference in spent with the novel object. Panels (B–D) shows results on the Closed field Symmetrical Maze comparing TW1 treated mice vs. vehicle-treated mice: (B) Day 1 two-tailed t -test *** p < 0.0001; (C) Day 2 two-tailed t -test ** p = 0.0015; (D) Day 3 two-tailed t -test *** p = 0.0002). ns: not significant.

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Two Tailed Test

Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: PHF1 Immunohistochemistry. TW1 passive immunotherapy reduces tau histopathology in hTau/PS1 mice model with PHF-1 PHF1 immunostaining. (A–C) Immunized animals had 38% less PHF-1 stained tau pathology in the motor cortex representing a trend for reduction (* p = 0.0448; one-tailed t -test). (D–F) TW1 treated mice showed a 50% reduction of PHF-1 immunoreactivity in the dentate gyrus (* p = 0.02; two-tailed t -test). (G–I) TW1 treated mice showed a 60% reduction of PHF-1 immunoreactivity in the piriform cortex (* p = 0.01; two-tailed t -test). Scale bar = 100 μm.

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Immunohistochemistry, Histopathology, Immunostaining, Staining, One-tailed Test, Two Tailed Test

Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: CP13 Immunohistochemistry. With CP13 immunostaining, no significant differences were observed in the motor cortex (A–C) and the dentate gyrus (D–F) . TW1 treated mice compared to vehicle controls showed reduced CP13 immunostaining in the piriform cortex (* p = 0.02; two-tailed t -test; G–I ). Scale bar = 100 μm.

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Immunohistochemistry, Immunostaining, Two Tailed Test

Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: No significant differences observed with GFAP and Iba-1 Immunostaining. The semi-quantitative analysis of GFAP immunoreactivity showed no significant differences comparing TW1 and vehicle-treated controls in the motor cortex (A–C) or in the dentate gyrus (D–F) . The semi-quantitative analysis of Iba-1 immunoreactivity showed no significant differences comparing TW1 and vehicle-treated controls in the motor cortex (G–I) or in the dentate gyrus (J–L) . Scale bar = 100 μm.

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Immunostaining

Journal: Frontiers in Aging Neuroscience

Article Title: Passive Immunization With a Novel Monoclonal Anti-PrP Antibody TW1 in an Alzheimer’s Mouse Model With Tau Pathology

doi: 10.3389/fnagi.2021.640677

Figure Lengend Snippet: TW1 passive immunotherapy reduces biochemical tau pathology in the hTau/PS1 mice model. Densitometric analysis of the blots from the soluble fraction indicates that an equal amount of tau protein was used in treated vs. control animals (A,D) . PHF-1 staining shows a decrease of pathological tau in treated animals compared to the controls (* p = 0.03; two-tailed t -test; B ). A significant reduction was also observed in the ratio of PHF1/Tubilin (*** p = 0.0006; two-tailed t -test; C ). With CP13 antibody immunoblotting a reduction of tau pathology in treated animals vs. controls is observed (*** p = 0.0007; two-tailed t -test; E ). A comparable effect was observed in the ratio CP13/Tubulin (** p = 0.0014; two-tailed t -test; F ).

Article Snippet: In brief, the TW1 mAb was purified from concentrated tissue-culture supernatant produced in Integra CL bioreactor flasks (Integra Biosciences, Chur, Switzerland).

Techniques: Staining, Two Tailed Test, Western Blot