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Image Search Results
Journal: The AAPS Journal
Article Title: Phosphorothioate Oligonucleotide Quantification by ?-Liquid Chromatography-Mass Spectrometry
doi: 10.1208/s12248-012-9381-2
Figure Lengend Snippet: Impact of the addition of ascorbic acid a to the mobile phase and b to the sample solvent on the detectability of oligonucleotides. Column, continuous styrene–divinylbenzene copolymer, 50 × 0.20 mm i.d.; mobile phase, a 25 mM CycHDMAA, pH 8.4, 0–570 μM ascorbic acid, b 25 mM CycHDMAA, pH 8.4, 100 % ACN, 0–570 μM ascorbic acid; linear gradient, 5 % B for 3 min, 5–95 % B in 7 min; flow rate, 2 μl/min; temperature, 70 °C; scan mode, MS; sample, PTO, 2 pmol, or (dT)24, 5 pmol, with 0–2.5 mM ascorbic acid
Article Snippet: The following oligonucleotides were purchased from
Techniques:
Journal: The AAPS Journal
Article Title: Phosphorothioate Oligonucleotide Quantification by ?-Liquid Chromatography-Mass Spectrometry
doi: 10.1208/s12248-012-9381-2
Figure Lengend Snippet: Reconstructed ion chromatograms of the PTO obtained with a SIM, b full scan MS/MS, and c SRM. Column, continuous styrene–divinylbenzene copolymer, 50 × 0.20 mm i.d.; mobile phase, (A) 25 mM CycHDMAA, pH 8.4, 100 μM ascorbic acid, (B) 25 mM CycHDMAA, pH 8.4, 100 % ACN, 100 μM ascorbic acid; linear gradient, 5 % B for 3 min, 5–95 % B in 7 min; flow rate, 2 μl/min; temperature, 70 °C; scan modes, a SIM, b MS/MS, c SRM; sample, PTO, 250 fmol, and (dT)24, 8 pmol
Article Snippet: The following oligonucleotides were purchased from
Techniques: Tandem Mass Spectroscopy
Journal: The AAPS Journal
Article Title: Phosphorothioate Oligonucleotide Quantification by ?-Liquid Chromatography-Mass Spectrometry
doi: 10.1208/s12248-012-9381-2
Figure Lengend Snippet: Reconstructed ion chromatograms of two plasma samples processed after the addition of 4 μM (dT)24 as well as a 1,000 nM or b 50 nM PTO, respectively. Column, continuous styrene–divinylbenzene copolymer, 50 × 0.20 mm i.d.; mobile phase, (A) 10 mM CycHDMAA, pH 8.4, 100 μM ascorbic acid, (B) 10 mM CycHDMAA, pH 8.4, 100 % ACN, 100 μM ascorbic acid; linear gradient, 5 % B for 3 min, 5–95 % B in 7 min; flow rate, 2 μl/min; temperature, 70 °C; scan mode, MS/MS; sample, processed plasma samples
Article Snippet: The following oligonucleotides were purchased from
Techniques: Tandem Mass Spectroscopy
Journal: Microbiology Spectrum
Article Title: Robust and sensitive amplicon-based whole-genome sequencing assay of respiratory syncytial virus subtype A and B
doi: 10.1128/spectrum.03067-23
Figure Lengend Snippet: RSV-AB/hMPV/IC multiplex real-time RT-PCR oligonucleotide sequences and final reaction concentrations
Article Snippet:
Techniques: Multiplex Assay, Quantitative RT-PCR, Sequencing, Concentration Assay, Amplification