Journal: Oncogene

Article Title: Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant

doi: 10.1038/s41388-019-1047-4

Figure Lengend Snippet: BMP7 is confined to differentiated CRC cells. a Immunofluorescence analysis of BMP7 (green color) and LGR5 (red color) on peritumoral mucosa and colon cancer paraffin-embedded tissues performed on CSC#8. One representative tumor from twenty different tumors examined is shown. Nuclei were counterstained by Toto-3 (blue color). White arrowheads indicate LGR5 + cells at the base of colon crypt. The scale bar represents 100 µm. b Immunohistochemical analysis of BMP7 on CRC TMAs in lack, low, medium, and high staining intensity (red color). Nuclei were counterstained by aqueous hematoxylin (blue color). The scale bar represents 100 µm. c Association of BMP7 expression with score medium/high and the pathological grading in CRC TMAs provided by TRISTAR technology group. d Immunohistochemical analysis of BMP7 (red color) in paraffin-embedded sections of colon adenomas and adenocarcinoma (COAD). Nuclei were counterstained by aqueous hematoxylin (blue color). The scale bar represents 100 µm. e Immunofluorescence analysis of BMP7 (green color) in CRC sphere cells and their differentiated progeny SDACs. One representative of fifteen different CR-CSC lines (CSC#1–3, 5–7, 10,11, 14–16, 18, 25, 33, and 40) is shown. Nuclei were counterstained by Toto-3 (blue color). The scale bars represent 20 µm. f Representative flow cytometry analysis of CD133 in CRC sphere cells and its relative isotype-matched control (IMC) (upper panels) performed on CSC#4, 8, and 23–26. Immunofluorescence analysis of BMP7 (green color) in CD133 + and CD133 − enriched CRC sphere cell subpopulations (lower panels). Nuclei were counterstained by Toto-3 (blue color). The scale bars represent 20 µm. g CD44v6 expression profiles of cells as described in f (upper panels). Expression of BMP7 (green color) in CD44v6 + and CD44v6 − enriched CRC sphere cell subpopulations assessed by immunofluorescence analysis (lower panels). Nuclei were counterstained by Toto-3 (blue color). The scale bars represent 20 µm. h Flow cytometry analysis of BMP7 (green histograms) in enriched CD44v6 − /CD133 − , CD44v6 − /CD133 + , and CD44v6 + /CD133 + CRC subpopulations performed as shown in f . Dotted line histograms indicate the relative IMC

Article Snippet: The scale bar represents 100 μm. c Association of BMP7 expression with score medium/high and the pathological grading in CRC TMAs provided by TRISTAR technology group. d Immunohistochemical analysis of BMP7 (red color) in paraffin-embedded sections of colon adenomas and adenocarcinoma (COAD).

Techniques: Immunofluorescence, Immunohistochemical staining, Staining, Expressing, Flow Cytometry, Control

Journal: Emerging Infectious Diseases

Article Title: Streptococcus gallolyticus and Bacterial Endocarditis in Swine, United States, 2015–2020

doi: 10.3201/eid2801.210998

Figure Lengend Snippet: Lesions associated with swine vegetative endocarditis, United States, 2015–2020. A) Macroscopic findings of vegetative growth on the left atrioventricular heart valve leaflets. B) Histopathologic findings of inflammation characterized by necrotic leukocytes (N), fibrin (F), mineralization (M), and myriad bacterial colonization (yellow outline) along the surface of the heart valve (hematoxylin and eosin staining); original magnification ×40. Higher magnification image (inset) shows cocci bacteria in clusters and long chains; original magnification ×1,000. C) Streptococcus gallolyticus directly detected (red) on the surface of the heart valve by RNA in situ hybridization with a probe targeting the helix-hairpin helix domain–containing protein, ComEC/Rec2, and DNA pol III subunit delta genes specific to S. gallolyticus ; original magnification ×40. Higher magnification image (inset) shows the bacteria labeled by the in situ hybridization probe; original magnification ×1,000.

Article Snippet: For selected cases from which pure growth of S. gallolyticus was isolated from the heart valves, we performed bacterial biochemical analyses, sequenced 16S ribosomal RNA, and localized S. gallolyticus within lesions via RNA in situ hybridization (ISH) (RNAscope; TriStar Technology LLC, https://tristargroup.us ).

Techniques: Staining, Bacteria, RNA In Situ Hybridization, Labeling, In Situ Hybridization

Journal: Translational Oncology

Article Title: Prognostic Significance and Functional Role of CEP57 in Prostate Cancer 1

doi: 10.1016/j.tranon.2015.11.004

Figure Lengend Snippet: High CEP57 expression is associated with more favorable BCR-free survival in two cohorts of prostate cancer patients. (A) Examples of immunohistochemical staining for CEP57 in two prostate cancer specimens with low or high protein expression, respectively. Scale bar = 250 μm. (B, C) Kaplan-Meier curves of BCR-free survival using two TMAs either obtained commercially (TMA I) or collected at the University of Bonn School of Medicine (TMA II). The log-rank test was used to calculate P values.

Article Snippet: Two tissue microarrays (TMAs) were used in this study: TMA I was obtained commercially (TriStar Technology Group, Washington, DC), and TMA II was constructed by the Institute of Pathology of the University of Bonn.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: PLoS ONE

Article Title: Upregulation of annexin A1 protein expression in the intratumoral vasculature of human non–small-cell lung carcinoma and rodent tumor models

doi: 10.1371/journal.pone.0234268

Figure Lengend Snippet: Vascular anxA1 staining in TMAs of NSCLC and normal tissues. Vascular anxA1 staining was positive in 30/80 NSCLC TMA samples, with 54/80 samples demonstrating neoplastic staining. Vascular staining in normal tissues was negative with exceptions of kidney medulla (2/2 positive vascular staining) fundus (3/3 positive vascular staining).

Article Snippet: Multitype human normal organ tissue microarrays (TMAs) (Tristar Technology Group, Rockville, MD) and NSCLC TMAs (US Biomax, Derwood, MD) were used in accordance with the ethical principles of the Declaration of Helsinki and in compliance with all national and local regulatory guidelines.

Techniques: Staining