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  • 99
    Thermo Fisher tris edta buffer te
    Tris Edta Buffer Te, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta buffer te/product/Thermo Fisher
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    tris edta buffer te - by Bioz Stars, 2020-08
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    99
    Millipore trisedta buffer
    Ethanol Precipitation Dissociates the Catalytic Subunit from the Regulatory Subunits of PP2A. The effect of ethanol precipitation on the molecular form of PP2A was determined following size exclusion chromatography in <t>Tris–EDTA</t> buffer containing 10 mM β -mercaptoethanol and 100 mM NaCl on a Sephacryl 300HR column of the crude S100 fraction (3.0 mg protein) and S100 fraction following ethanol precipitation, extraction, and ammonium sulfate precipitation to form the EtOH-AS65 fraction (0.15 mg protein). PP2Ac in the fractions was determined by measuring the phosphatase activity toward a phosphothreonine peptide. Phosphatase activity is reported as nmol Pi produced during a 10 min incubation period per 225 μL of the column fractions. γ -Globulin (158 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa) were employed as molecular weight markers
    Trisedta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    trisedta buffer - by Bioz Stars, 2020-08
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    92
    Thermo Fisher tris edta buffer
    Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: <t>Tris-EDTA)</t> fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  
    Tris Edta Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta buffer/product/Thermo Fisher
    Average 92 stars, based on 788 article reviews
    Price from $9.99 to $1999.99
    tris edta buffer - by Bioz Stars, 2020-08
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    99
    Thermo Fisher tbe buffer
    Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, <t>0.5×</t> <t>TBE,</t> 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .
    Tbe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbe buffer/product/Thermo Fisher
    Average 99 stars, based on 2279 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2020-08
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    93
    Thermo Fisher buffer
    Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, <t>0.5×</t> <t>TBE,</t> 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .
    Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 29794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer/product/Thermo Fisher
    Average 93 stars, based on 29794 article reviews
    Price from $9.99 to $1999.99
    buffer - by Bioz Stars, 2020-08
    93/100 stars
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    Image Search Results


    Ethanol Precipitation Dissociates the Catalytic Subunit from the Regulatory Subunits of PP2A. The effect of ethanol precipitation on the molecular form of PP2A was determined following size exclusion chromatography in Tris–EDTA buffer containing 10 mM β -mercaptoethanol and 100 mM NaCl on a Sephacryl 300HR column of the crude S100 fraction (3.0 mg protein) and S100 fraction following ethanol precipitation, extraction, and ammonium sulfate precipitation to form the EtOH-AS65 fraction (0.15 mg protein). PP2Ac in the fractions was determined by measuring the phosphatase activity toward a phosphothreonine peptide. Phosphatase activity is reported as nmol Pi produced during a 10 min incubation period per 225 μL of the column fractions. γ -Globulin (158 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa) were employed as molecular weight markers

    Journal: Neurochemical research

    Article Title: Phenylarsine Oxide Binding Reveals Redox-Active and Potential Regulatory Vicinal Thiols on the Catalytic Subunit of Protein Phosphatase 2A

    doi: 10.1007/s11064-010-0310-4

    Figure Lengend Snippet: Ethanol Precipitation Dissociates the Catalytic Subunit from the Regulatory Subunits of PP2A. The effect of ethanol precipitation on the molecular form of PP2A was determined following size exclusion chromatography in Tris–EDTA buffer containing 10 mM β -mercaptoethanol and 100 mM NaCl on a Sephacryl 300HR column of the crude S100 fraction (3.0 mg protein) and S100 fraction following ethanol precipitation, extraction, and ammonium sulfate precipitation to form the EtOH-AS65 fraction (0.15 mg protein). PP2Ac in the fractions was determined by measuring the phosphatase activity toward a phosphothreonine peptide. Phosphatase activity is reported as nmol Pi produced during a 10 min incubation period per 225 μL of the column fractions. γ -Globulin (158 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa) were employed as molecular weight markers

    Article Snippet: For each S100 fraction prepared, one whole brain was partially thawed and homogenized in a glass-Teflon homogenizer in 15 mL of Tris–EDTA buffer (50 mM Tris, 1 mM EDTA, 1 mM benzamidine, pH 7.4) to which was added 50 μL of mammalian protease inhibitor cocktail (Sigma Chemical) per brain.

    Techniques: Ethanol Precipitation, Size-exclusion Chromatography, Activity Assay, Produced, Incubation, Molecular Weight

    Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: Tris-EDTA) fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  

    Journal: Toxicological Sciences

    Article Title: Heavy Metal Neurotoxicants Induce ALS-Linked TDP-43 Pathology

    doi: 10.1093/toxsci/kfy267

    Figure Lengend Snippet: Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: Tris-EDTA) fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  

    Article Snippet: A total of ∼100 mg of frozen cortical tissue was lysed by bead mill (Thermo) homogenization in Tris-EDTA buffer (10 mM Tris pH8; 1 mM EDTA; 1 mM PMSF; 1× HALT PIC, and PhosSTOP [Roche]).

    Techniques: Mouse Assay, Solubility, Molecular Weight, Two Tailed Test

    Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, 0.5× TBE, 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .

    Journal: Nature

    Article Title: ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference

    doi: 10.1038/nature23671

    Figure Lengend Snippet: Characterization of barcoded 601 (BC-601) DNA a , BC-601 DNA prepared for all 115 nucleosome library members as described in Methods (Barcoded 601 (BC-601) DNA preparation). Ligation products are 192 bp in size and were visualized by polyacrylamide gel electrophoresis (5% acrylamide, 0.5× TBE, 200 V, 40 min) and staining with SYBR Safe DNA gel stain. A faint band corresponding to unligated 601 DNA (601) is slightly visible in certain cases. b .

    Article Snippet: Quenched assay samples were directly run on a 5% TBE gel in 0.5× TBE buffer for 40 min at 200 V. Nucleosomes were visualized by staining with SYBR Gold Nucleic Acid Gel Stain (ThermoFisher Scientific).

    Techniques: Ligation, Polyacrylamide Gel Electrophoresis, Staining