tris-edta buffer Search Results


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  • 99
    Thermo Fisher tbe buffer
    Tbe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbe buffer/product/Thermo Fisher
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    99
    Millipore edta
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 35690 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    edta - by Bioz Stars, 2021-01
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    99
    Millipore tris edta buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Edta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tris edta buffer - by Bioz Stars, 2021-01
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    99
    Thermo Fisher tae buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tae Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tae buffer - by Bioz Stars, 2021-01
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    99
    Thermo Fisher tris edta buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Edta Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tris edta buffer - by Bioz Stars, 2021-01
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    92
    Bio-Rad tris borate edta buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Borate Edta Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1068 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tris borate edta buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Borate Edta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies tris edta buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Edta Buffer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 571 article reviews
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    99
    Millipore tris acetate edta buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Acetate Edta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher tris edta te buffer
    Soluble rCTD is a dimer in equilibrium and the <t>CTD</t> cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM <t>Tris,</t> 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.
    Tris Edta Te Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher tris acetate edta tae buffer
    Electropherogram of PCR fragments in 1.5% agarose gel in a <t>tris-acetate-EDTA</t> buffer. Scale to the left: the corresponding number of base pairs. CRP expression was detected in: HepG2—human cell line of hepatocellular carcinoma; HepG2-IL6—interleukin-6-stimulated HepG2 cells; GM-CSF, LPS-stimulated monocytes—granulocyte-macrophage colony-stimulating factor and lipopolysaccharide-stimulated peripheral blood monocytes; b.p.—base pairs.
    Tris Acetate Edta Tae Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris edta buffer solution
    Electropherogram of PCR fragments in 1.5% agarose gel in a <t>tris-acetate-EDTA</t> buffer. Scale to the left: the corresponding number of base pairs. CRP expression was detected in: HepG2—human cell line of hepatocellular carcinoma; HepG2-IL6—interleukin-6-stimulated HepG2 cells; GM-CSF, LPS-stimulated monocytes—granulocyte-macrophage colony-stimulating factor and lipopolysaccharide-stimulated peripheral blood monocytes; b.p.—base pairs.
    Tris Edta Buffer Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 611 article reviews
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    tris edta buffer solution - by Bioz Stars, 2021-01
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    96
    Bio-Rad tae buffer
    Electropherogram of PCR fragments in 1.5% agarose gel in a <t>tris-acetate-EDTA</t> buffer. Scale to the left: the corresponding number of base pairs. CRP expression was detected in: HepG2—human cell line of hepatocellular carcinoma; HepG2-IL6—interleukin-6-stimulated HepG2 cells; GM-CSF, LPS-stimulated monocytes—granulocyte-macrophage colony-stimulating factor and lipopolysaccharide-stimulated peripheral blood monocytes; b.p.—base pairs.
    Tae Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1874 article reviews
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    tae buffer - by Bioz Stars, 2021-01
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    99
    Abcam tris edta buffer
    Electropherogram of PCR fragments in 1.5% agarose gel in a <t>tris-acetate-EDTA</t> buffer. Scale to the left: the corresponding number of base pairs. CRP expression was detected in: HepG2—human cell line of hepatocellular carcinoma; HepG2-IL6—interleukin-6-stimulated HepG2 cells; GM-CSF, LPS-stimulated monocytes—granulocyte-macrophage colony-stimulating factor and lipopolysaccharide-stimulated peripheral blood monocytes; b.p.—base pairs.
    Tris Edta Buffer, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 119 article reviews
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    tris edta buffer - by Bioz Stars, 2021-01
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    N/A
    Tris EDTA Buffer Liquid is a 100X concentrate that when diluted to 1X contains 10 mM Tris 1 mM EDTA pH 8 0 Based on nuclease studies from the 1980
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    N/A
    suitable for gel electrophoresis after dilution to working concentration 0 8 M Tris phosphate pH approx 8 0 0 02 M EDTA
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    N/A
    10X Tris Phosphate EDTA TPE 0 9 M Tris Phosphate 0 01 M EDTA pH 8 0 MB 021
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    N/A
    10X Tris Borate EDTA TBE 0 9 M Tris Borate 0 01 M EDTA pH 8 3 MB 019
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    Image Search Results


    Soluble rCTD is a dimer in equilibrium and the CTD cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM Tris, 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.

    Journal: Scientific Reports

    Article Title: The outer-membrane export signal of Porphyromonas gingivalis type IX secretion system (T9SS) is a conserved C-terminal β-sandwich domain

    doi: 10.1038/srep23123

    Figure Lengend Snippet: Soluble rCTD is a dimer in equilibrium and the CTD cleaved off natively expressed proRgpB spontaneously dimerizes. ( A ) rCTD at 1, 0.5 and 0.1 mg ml −1 was treated with glutaraldehyde and analysed by SDS-PAGE. ( B ) Recombinant CTD (rCTD) (red), proRgpB662iXa (black), and proRgpB662iXa preincubated with fXa (blue) were subjected to size exclusion chromatography on a Superdex 75 10/300 GL column equilibrated with 50 mM Tris, 150 mM NaCl, 2.5 mM CaCl 2 , 0.02% NaN 3 pH 7.5 ( C ) Indicated fractions of resolved proteins were analysed by Western blot using anti-rCTD antibodies to reveal the CTD content in each analysed fraction.

    Article Snippet: Limited proteolysis investigations Different rIgSF-CTD variants were incubated with tested proteases in 20 mM Tris, 50 mM NaCl, 5 mM CaCl2 , pH 7.6 (bovine factor Xa (Sigma-Aldrich, St. Louis, MO, USA)), 25 mM Tris, 1 mM EDTA, 0.02% NaN3 , pH 8.0 (Lys-C endopeptidase and V8 protease (Promega, Fitchburg, WI, USA)) or 0.1 M Tris, 150 mM NaCl, 5 mM EDTA, 0.05% Tween 20, 0.02% NaN3 , pH 7.5 (porcine pancreatic elastase (Promega)) and human neutrophil elastase (BioCentrum, Krakow, Poland)) for up to 72 h at different weight ratios.

    Techniques: SDS Page, Recombinant, Size-exclusion Chromatography, Western Blot

    Electropherogram of PCR fragments in 1.5% agarose gel in a tris-acetate-EDTA buffer. Scale to the left: the corresponding number of base pairs. CRP expression was detected in: HepG2—human cell line of hepatocellular carcinoma; HepG2-IL6—interleukin-6-stimulated HepG2 cells; GM-CSF, LPS-stimulated monocytes—granulocyte-macrophage colony-stimulating factor and lipopolysaccharide-stimulated peripheral blood monocytes; b.p.—base pairs.

    Journal: Biomedicines

    Article Title: CRP Is Transported by Monocytes and Monocyte-Derived Exosomes in the Blood of Patients with Coronary Artery Disease

    doi: 10.3390/biomedicines8100435

    Figure Lengend Snippet: Electropherogram of PCR fragments in 1.5% agarose gel in a tris-acetate-EDTA buffer. Scale to the left: the corresponding number of base pairs. CRP expression was detected in: HepG2—human cell line of hepatocellular carcinoma; HepG2-IL6—interleukin-6-stimulated HepG2 cells; GM-CSF, LPS-stimulated monocytes—granulocyte-macrophage colony-stimulating factor and lipopolysaccharide-stimulated peripheral blood monocytes; b.p.—base pairs.

    Article Snippet: After the initial denaturation stage (950, 10 min), 40 amplification cycles were performed for all primer pairs with annealing and elongation at 600 °C for 60 s. The specificity of amplification was analyzed by melting the products after completion of the PCR, as well as by electrophoresis of amplicons in 1.5% agarose gel in a tris-acetate-EDTA (TAE) buffer (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Expressing