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Synaptic Systems
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Proteintech
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Biorbyt
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Synaptic Systems
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Synaptic Systems
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Atlas Antibodies
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EUROIMMUN
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Shanghai GenePharma
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Shanghai Genechem Ltd
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TRIM46 GFP tagged Human tripartite motif containing 46 TRIM46 transcript variant 8
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Rabbit polyclonal antibody against TRIM46 conjugated to FITC Isotype Note: IgG Host Note: Rabbit Conjugation Note: FITC Reactivity Note: Human, Mouse, Rat Application Note: IF/ICC
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Image Search Results
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A) TRIM46 immunostaining in wildtype adult sciatic nerve, ventral root, and dorsal root. (B) Immunostaining for Caspr (blue), AnkG (green), and TRIM46 (red) in wildtype proximal sciatic nerve. (C) TRIM46 immunostaining in wildtype corpus callosum. (D-E) Immunostaining for Caspr (blue), AnkG (green), and TRIM46 (red) in (D) dorsal root from adult Ank3 F/F and Adv-Cre;Ank3 F/F mice and (E) DRG from adult wildtype and Trim46 -/- mice. Scale bars: 10 μm.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques: Immunostaining
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A) Design of knockout allele. (B) Images of 1-month old wildtype and Trim46 -/- mice. (C) PCR of genomic DNA. (D) Pie chart of genotypes obtained from crossing Trim46 +/- mice (n=275). (E) Sanger sequencing of genomic DNA at the start and end of exon 3. (F-M) Validation of TRIM46 knockout by immunostaining (F-I) and immunoblotting (J-M) using four different TRIM46 antibodies: two monoclonal rabbit antibodies (#1: F, J; #2: G, K), a polyclonal guinea pig antibody (H, L), and a monoclonal guinea pig antibody (I, M). (F-I) Immunostaining of TRIM46 (green) and NeuN (magenta, shown only in the merged images) in adult cortex. (J-M) Immunoblotting of 20 μg (J, K, M) or 30 μg (L) crude synaptosomal fraction of adult brain. Scale bars: (B) 1 cm, (F-I) 10 μm.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques: Knock-Out, Sequencing, Biomarker Discovery, Immunostaining, Western Blot
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A-B) Epitope mapping of the four TRIM46 antibodies used in , summarized in (A) with representative images in (B) (red: Myc tag on TRIM46 construct, green: TRIM46 antibody). (A) Also indicates microtubule localization of each construct. (C) Immunostaining showing subcellular distribution of various TRIM46 constructs. Scale bars: 50 μm.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques: Construct, Immunostaining
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A) Immunostaining of NeuN (blue), TRIM46 (green), and AnkG (red) in adult cortex. (B-D) Quantification of AIS in adult cortex of (B) percentage of neurons with an AnkG+ AIS (n=3 mice, N=211-422 neurons per mouse), (C) fluorescence intensity of AnkG at the AIS, and (D) AIS length (based on AnkG staining) (N=38 WT and 26 Trim46 -/- AIS from n=1 mouse for C-D). (B) was analyzed with one-way ANOVA (p=0.7886) with Tukey’s multiple comparisons test (adjusted p-values shown on graph). (C-D) were analyzed with unpaired t-tests. (E) Immunostaining of AnkG and βIV-spectrin in cortex at postnatal day 0 (n=2). Error bars represent SEM. Scale bars: 10 μm.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques: Immunostaining, Fluorescence, Staining
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A) Latency to fall on accelerating rotarod at 3-5 months of age (males, n=8; females, n=10 WT, 7 Trim46 +/- , 8 Trim46 -/- ). The first point for Trim46 +/- males lacks an error bar because the data were too close together to display one. Two-way ANOVA: for males, p genotype <0.0001, p trial =0.0001, p genotype*trial =0.0531; for females, p genotype <0.0001, p trial <0.0001, p genotype*trial =0.6068. (B) Body weight of the mice tested on rotarod and wire hang. (C) Average latency to fall on wire hang test at 3-5 months of age. (D, E) Gait analysis at 3-4 months of age. (B, C, E) were analyzed with two-way ANOVA (p-values: (B) p genotype =0.1367, (C) p genotype =0.3058, (E) p genotype =0.0466) with Tukey’s multiple comparisons test (adjusted p-values shown on graphs). Error bars represent SEM.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques:
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A) Motor neurons in Ank3 F/F and ChAT-Cre;Ank3 F/F spinal cord immunostained for NeuN (blue), AnkG (green), and TRIM46 (red). (B) Examples of TRIM46 immunostaining in ChAT-Cre;Ank3 F/F spinal cord. (C) Quantification of the length of proximal axon immunopositive for TRIM46 ( Ank3 F/F mean=23.52 μm, ChAT-Cre;Ank3 F/F mean= 94.58 μm, N=26-52 axons per mouse, n=3 mice). Analyzed with unpaired t-test. (D) Confocal microscopy of TRIM46 in Ank3 F/F and ChAT-Cre;Ank3 F/F spinal cord. Error bars represent SEM. Scale bars: (A, B) 30 μm, (C) 10 μm.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques: Immunostaining, Confocal Microscopy
Journal: bioRxiv
Article Title: TRIM46 is not required for axon specification or axon initial segment formation in vivo
doi: 10.1101/2024.05.23.595556
Figure Lengend Snippet: (A) Immunostaining for TRIM46 (red), AnkG (green), and MAP2 (blue) in cultured rat hippocampal neurons transduced with Cas9 and control or TRIM46 gRNA at DIV 0 and fixed at DIV 21. (B-C) Quantification from cultured rat cortical and hippocampal neurons transduced with Cas9 and control or TRIM46 gRNA between DIV 0-DIV 5 and fixed at DIV 17-DIV 21 (n=4, N=195-266 neurons pooled from 2-4 coverslips per replicate). (B) Percentage of neurons with a TRIM46-positive AIS in control (mean: 98.25%) and TRIM46 (mean: 6.5%) gRNA neurons. Analyzed with unpaired t-test. (C) Percentage of neurons with an AnkG-positive AIS in control (mean: 97.25%) and TRIM46 (mean: 93.5%) gRNA cultures. On the right, only TRIM46-negative neurons in the TRIM46 gRNA cultures are included (mean: 93.25%; N=193-250 neurons per replicate). Analyzed with ordinary one-way ANOVA (P=0.0501) with Dunnett’s multiple comparisons test (adjusted p-values shown on graph). (D) Immunostaining for TRIM46 (red), AnkG (green), and MAP2 (blue, shown only in the merged images) in cultured rat cortical neurons that were transduced with Cas9 and TRIM46 gRNA at DIV 1 and fixed at DIV 21. The neurons on the right were treated with Triton X-100 before fixation. Each field of view includes both TRIM46-negative (arrows) and TRIM46-positive (arrowheads) neurons for comparison purposes. (E) Immunostaining for TRIM46 (red), AnkG (green), and MAP2 (blue, shown only in the merged images) in cultured rat hippocampal neurons that were transduced with Cas9 and AnkG gRNA at DIV 0 and fixed at DIV 21. The neurons on the right were treated with Triton X-100 before fixation. Each field of view includes both AnkG-negative (arrows) and AnkG-positive (arrowheads) neurons for comparison purposes.
Article Snippet: Rasband, Baylor College of Medicine, Texas, USA Cat# Anti-Caspr, RRID:AB_2572297, 1:250-1:500), CUX1 (Santa Cruz Biotechnology Cat# sc-13024, RRID:AB_2261231, 1:100), Na v 1.6 (Alomone Labs Cat# ASC-009, RRID:AB_2040202, 1:500), and NDEL1 (Thermo Fisher Scientific Cat# PA5-53669, RRID:AB_2644530, 1:200), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308, RRID:AB_2924929, 1:1000),
Techniques: Immunostaining, Cell Culture, Transduction, Control, Comparison
Journal: Cell Reports
Article Title: MAP7D2 Localizes to the Proximal Axon and Locally Promotes Kinesin-1-Mediated Cargo Transport into the Axon
doi: 10.1016/j.celrep.2019.01.084
Figure Lengend Snippet:
Article Snippet: The following antibodies were used for the immunofluorescence staining: mouse anti-AnkG (Neuromab, 75-146);
Techniques: Recombinant, CRISPR, Knock-Out, shRNA, Sequencing, Software