Journal: International Journal of Molecular Sciences

Article Title: Transglutaminase 2 Facilitates Murine Wound Healing in a Strain-Dependent Manner

doi: 10.3390/ijms241411475

Figure Lengend Snippet: mRNA abundance of wound-related genes was increased to the same levels in 129 TG2 −/− and 129 TG2 +/+ mice on day 2 post-wounding. cDNA generated from total RNA isolated from unwounded skin samples ( n = 3 per genotype) or skin samples on day 2 post-wounding ( n = 3 per genotype) was examined in triplicate for the abundance of mRNAs for ( A ) TLR pathway genes Ticam1 (encoding TICAM1), Myd88 (encoding MyD88), Nfkb1 (encoding NF-κB subunit 1), Nfkbib (encoding I-κBβ), ( B ) early inflammatory cytokines Il6 (encoding IL6), Ifng (encoding IFN-γ) and Tnf (encoding TNF-α), and growth factors Egf (encoding EGF), Fgf2 (encoding basic fibroblast growth factor) and Tgfb2 (encoding TGFβ2), ( C ) ECM-related genes Fndc4 (encoding Fibronectin Type III Domain-Containing Protein 4), Fndc3a (encoding the wound-related fibronectin extra-domain A splice variant), Col1a1 (encoding collagen type I α1 chain), Col1a2 (encoding collagen type I α2 chain), Col3a1 (encoding collagen type III α1 chain), Sdc4 (encoding syndecan-4), ( D ) TG family members Tgm1 (encoding TG1), Tgm2 (encoding TG2), Tgm3 (encoding TG3), Tgm4 (encoding TG4), Tgm5 (encoding TG5), Tgm6 (encoding TG6), Tgm7 (encoding TG7), f13a (encoding F13A), normalized to Hprt (encoding hypoxanthine phosphoribosyltransferase 1) as the reference gene (expression level unaffected by the experimental treatment). *, p < 0.05; **, p < 0.01; ***, p < 0.001 for two tailed Student’s t test between respective different treatment groups of the same genotype. ###, p < 0.001 for two tailed Student’s t test between different genotypes in the same respective treatment group.

Article Snippet: Quantitative PCR was performed in triplicate to examine the expression levels of Tgm1 (assay ID: Mm00498375_m1*), Tgm2 (Mm00436987_m1*), Tgm3 (Mm00436999_m1*), F13a1 (Mm00472334_m1*), Tgm4 (Mm00626039_m1*), Tgm5 (Mm00551325_m1*), Tgm6 (Mm00624922_m1*), Tgm7 (Mm03990491_m1*), Il6 (Mm00446190_m1*), Ifng (Mm00497611_m1*), Tnf (Mm00443258_m1*), Egf (Mm00438696_m1*), Fgf2 (Mm00433287_m1*), Tgfb2 (Mm00436955_m1*), Ticam1 (Mm00844508_s1*), Myd88 (Mm00440338_m1*), Nfkb1 (Mm00476361_m1*), Nfkbib (Mm00456849_m1*), Fndc4 (Mm00480765_m1*), Fndc3a (Mm01232694_m1*), Col1a1 (Mm00801666_g1*), Col1a2 (Mm00483888_m1*), Col3a1 (Mm01254476_m1*), Sdc4 (Mm00488527_m1*), with Hprt (Mm00446968_m1*) as the most suitable reference gene of four reference genes ( Hprt , GAPDH , 18S rRNA and β-2-microglubin ( B2M )) tested (expression unaffected by the experimental treatment).

Techniques: Generated, Isolation, Variant Assay, Gene Expression, Two Tailed Test

Journal: Molecules

Article Title: Hepatoprotective Effects of Kaempferol-3-O-α-l-Arabinopyranosyl-7-O-α-l-Rhamnopyranoside on d-Galactosamine and Lipopolysaccharide Caused Hepatic Failure in Mice

doi: 10.3390/molecules22101755

Figure Lengend Snippet: Figure 7. The expression of NF-κB and TLR4 signaling pathway-related proteins, such as NF-Kb (A); IκBα (B); p65 (C); TLR4 (D); TRAF6 (E); MyD88 (F); and TRIF (G); were detected using a Western blotting assay in the liver tissues of each group 6 h after D-GalN/LPS administration. The data represented show the mean ± SD. Data of IκBα, p65, and NF-κB are normalized to that of Lamin B, and that of TLR4, TRAF6, MyD88, and TRIF are normalized to β-actin. * p < 0.05, ** p < 0.01 compared to the FHF model group, and # p < 0.05, ## p < 0.01 compared to the control group.

Article Snippet: After blocking in 5% (w/v) non-fat dry milk in PBST for 1 h, the membranes were incubated with primary antibodies for detecting cytochrome C, Bcl-2, Bax, caspase-3, caspase-8, caspase-9, TLR4, TRIF, TAF6, MyD88, p65, NF-κB, and Iκ-Bα (Cell Signaling Technology, Danvers, MA,USA) overnight at 4 °C.

Techniques: Expressing, Western Blot, Control