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Image Search Results
Journal: Scientific Reports
Article Title: De novo deoxyribonucleotide biosynthesis regulates cell growth and tumor progression in small-cell lung carcinoma
doi: 10.1038/s41598-021-92948-9
Figure Lengend Snippet: RRM1 expression and sensitivity to RRM1 inhibitors. A and B , mRNA expression of RNR-related genes in DMS 273 ( A ) and NCI-H1048 ( B ) cells. RRM1 (red) , RRM2 (blue), and RRM2B (green) levels were normalized to glyceraldehyde 3‐phosphate dehydrogenase ( GAPDH ) expression. Data represent the mean ± standard deviation (SD; n = 3). ( C – F ), WST-8 assay with the RRM1 inhibitors COH29 ( C ), triapine ( D ), hydroxyurea ( E ), and gemcitabine ( F ). Cells were treated with the indicated inhibitors for 3 days and their viability was assessed using the WST-8 assay. Red circle: DMS 273, blue square: H1048. Data represent the mean ± SD ( n = 6–10).
Article Snippet: DMS 273 and NCI-H1048 cells were seeded in 96-well cell culture plates containing RPMI 1640 with various concentrations of the following inhibitors: COH29,
Techniques: Expressing, Standard Deviation