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  • 90
    Alomone Labs triacsin c
    ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM <t>triacsin</t> <t>C</t> (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).
    Triacsin C, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
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    86
    Enzo Biochem triacsin c
    ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM <t>triacsin</t> <t>C</t> (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).
    Triacsin C, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Enzo Biochem
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
    86/100 stars
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    86
    Santa Cruz Biotechnology triacsin c
    ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM <t>triacsin</t> <t>C</t> (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).
    Triacsin C, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
    86/100 stars
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    86
    Fermentek Ltd triacsin c
    ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM <t>triacsin</t> <t>C</t> (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).
    Triacsin C, supplied by Fermentek Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Fermentek Ltd
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
    86/100 stars
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    86
    Millipore triacsin c
    ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM <t>triacsin</t> <t>C</t> (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).
    Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
    86/100 stars
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    Image Search Results


    ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM triacsin C (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).

    Journal: Scientific Reports

    Article Title: Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases

    doi: 10.1038/srep25469

    Figure Lengend Snippet: ( a , b ) IEC-6 cells were incubated with 5 μM [ 3 H]DHS ( a ) or [ 3 H]palmitic acid (PAL) ( b ) for the indicated time period. Radioactivities associated with cells and medium were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. ( c , d ) IEC-6 cells were labeled with 5 μM [ 3 H]DHS ( c ) or 5 μM [ 3 H] palmitic acid (PAL; d ) in the presence of cold palmitic acid or DHS, respectively, at the indicated concentration at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01). ( e , f ) BY4741 (wild-type; WT) or AOY13 ( faa1 Δ faa4 Δ; ΔΔ) cells harboring the pAKNF316 (vector; vec), pAO15 ( 3xFLAG-ACSL1 ; L1), pAO16 ( 3xFLAG-ACSL3 ; L3), pAO17 ( 3xFLAG-ACSL4 ; L4), pAO19 ( 3xFLAG-ACSL5 ; L5), or pAO21 ( 3xFLAG-ACSL6 ; L6) plasmid were grown in SC-URA medium at 30 °C. ( e ) Cells were labeled with 20 μM [ 3 H]DHS at 30 °C for 5 min. Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments. Statistically significant differences with respect to the value of AOY13 ( faa1 Δ faa4 Δ) cells harboring vector are indicated ( t -test; ** p < 0.01). ( f ) Total cell lysates were prepared, separated by SDS-PAGE, and subjected to immunoblotting with anti-FLAG or, to demonstrate equal protein loading, anti-Pgk1 antibody. ( g , h ) IEC-6 cells were treated with DMSO or 20 μM triacsin C (Tri C) at 37 °C for 1 hr. Cells were then labeled with 5 μM [ 3 H] palmitic acid ( g ) or 5 μM [ 3 H]DHS ( h ) at 37 °C for 15 min. Transport activity was determined as in ( a ). Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated ( t -test; * p < 0.05; ** p < 0.01).

    Article Snippet: Triacsin C was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Incubation, Radioactivity, Labeling, Concentration Assay, Activity Assay, Plasmid Preparation, SDS Page, Western Blot