Journal: Frontiers in Pediatrics

Article Title: Prevalence of osteopathologies in a single center cohort of survivors of childhood primary brain tumor

doi: 10.3389/fped.2022.913343

Figure Lengend Snippet: Descriptive statistics of the cohort and parameters of bone health.

Article Snippet: Plasma levels of osteoprotegerin (pmol/l), receptor activator of nuclear factor kappa-B ligand (RANKL, pmol/l), and tartrate-resistant acid phosphatase 5b (TRAP5b, U/l) were measured using commercially available ELISA assays, according to the manufacturer’s instructions (Osteoprotegerin: OPG, Quidel ® ; RANKL: sRANKL, BIOMEDICA and TRAP 5b: TRAP5b, Quidel ® ; all TECOmedical, Germany).

Techniques:

Journal: Biomaterials

Article Title: Local pulsatile PTH delivery regenerates bone defect via enhanced bone remodeling in a cell-free scaffold

doi: 10.1016/j.biomaterials.2016.10.049

Figure Lengend Snippet: Analysis of the systemic effects of PTH release from the local delivery devices. (A) Representative μCT reconstructions of the tibiae from different treatment groups; (B) Trabecular bone volume; (C) Serum P1NP level and (D) TRAP5b level measured using ELISA kits. n=6~9 per group, *P<0.05, **P<0.005, #P<0.05, ##P<0.005, ###P<0.001.

Article Snippet: Serum procollagen I N-terminal propeptide (P1NP) (MyBioSource, Inc) and TRAP5b (Novatein Biosciences, MA) ELISA immunoassays were performed following the manufacturer’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Thyroid

Article Title: Biochemical Markers Reflecting Thyroid Function in Athyreotic Patients on Levothyroxine Monotherapy

doi: 10.1089/thy.2016.0426

Figure Lengend Snippet: Thyroid Hormone Levels and Bone Metabolic Markers Before and After Thyroidectomy in the Patients Whose Serum Intact PTH Levels Were >10 pg/mL One Day After Thyroidectomy

Article Snippet: Serum tartrate-resistant acid phosphatase (TRACP)-5b was measured as a marker of bone resorption using an enzyme-linked immunosorbent assay (Nittobo Medical, Tokyo, Japan).

Techniques:

Journal: PLoS ONE

Article Title: The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class–Effect Synergy in Experimental Arthritis

doi: 10.1371/journal.pone.0054299

Figure Lengend Snippet: Fasted rats were given a single dose of Dexamethasone (15 or 100 µg/kg i.p.) with or without 1.0 µg/kg elcatonin (eCT). Blood was collected by venepuncture and glucose was quantified: i) immediately prior to fasting, ii) prior to drug treatment, and iii) 5 h after drug treatment. Overnight fasting caused a fall in mean blood glucose from 6.19±0.10 to 4.80±0.13 mM. Blood glucose in the vehicle-treated group continued to drop, reaching 3.86±0.22 mM at the 5-hour time point. (A) Blood glucose data as measured 5 h post-treatment, and normalised for differing pre-fasting levels. (B) ACTH was assayed by EIA in blood collected by terminal cardiac puncture at 5 h post-treatment. ACTH suppression is expressed in relation to the levels quantified by ELISA in vehicle-treated animals (1.57±0.09 ng/ml). In both panels, data are mean ± SEM of 8 rats per group. Statistical analyses by one-way ANOVA (Kruskal-Wallis test with Dunn’s post-test); *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated group, or by Mann-Whitney test; † p>0.05 in the indicated comparison.

Article Snippet: The following markers were assayed using commercially available kits: MMP-2, CXCL5, CCL5, CCL20, CXCL7 ELISA from R&D Systems (Abingdon, UK); telopeptide of type-I collagen (CTX-I) EIA from Immunodiagnostic Systems (Tyne & Wear, UK); TRAP-5b and ACTH EIA from Biosupply UK Ltd. (Bradford, UK).

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Cell

Article Title: Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption

doi: 10.1016/j.cell.2021.02.002

Figure Lengend Snippet: Regulation of osteoclast recycling by RANKL signaling (A) Raw images of LYSM + BLIMP1 + osteoclasts from mice treated with vehicle (n = 9), sRANKL (n = 6), OPG:Fc (n = 8), or following OPG:W (n = 6). Scale bar, 60 μm. (B) Pseudocolor density plot of volume and sphericity for double-labeled cells that have taken up fluorescent bisphosphonate analyzed in (A). (C) Network analysis using FilamentTracer of cells in (A). Percentage of cells in each quadrant are shown. (D) Sum of network lengths and the proportion of networks >1 mm in length from (C). Each data point represents one mouse. Mean ± SEM are shown. (E) Longitudinal changes in serum TRAP 5b in mice treated with vehicle (n = 9) and following OPG:W (n = 8). Mean ± SEM are shown. (F) Longitudinal changes in BMD in mice treated with vehicle and OPG:W. Mean ± SEM are shown. (G) Micro-computed tomography (CT) images of cancellous bone in the femur of mice treated with vehicle (n = 9), sRANKL (n = 7), OPG:Fc (n = 7), and OPG:W at 5 (n = 6), 8 (n = 8), 12 (n = 6), and 17 (n = 7) weeks (w). Scale bar, 200 μm. (H) Trabecular bone volume/tissue volume (BV/TV) calculated from (G). (I) Histological images of the femur stained for TRAP (red) showing osteoclasts (arrow heads) in mice treated with vehicle (n = 8), sRANKL (n = 5), OPG:Fc (n = 6), and OPG:W at 5 (n = 7), 8 (n = 7), 12 (n = 6), and 17 (n = 7) w. BM, bone marrow; GP, growth plate. Scale bar, 50 μm. (J) Number of TRAP + osteoclasts per mm of bone surface. Each data point represents one mouse. Mean ± SEM are shown. See also Figure S2 .

Article Snippet: Serum TRAP 5b ELISA – Mouse TRAPTM , Abacus ALS Pty Ltd , Cat#: IDSBTR103.

Techniques: Labeling, Micro-CT, Staining

Journal: Cell

Article Title: Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption

doi: 10.1016/j.cell.2021.02.002

Figure Lengend Snippet:

Article Snippet: Serum TRAP 5b ELISA – Mouse TRAPTM , Abacus ALS Pty Ltd , Cat#: IDSBTR103.

Techniques: Recombinant, Imaging, Construct, Enzyme-linked Immunosorbent Assay, DNA Library Preparation, Sequencing, Software

Journal: International Journal of Molecular Sciences

Article Title: Immobilization of Denosumab on Titanium Affects Osteoclastogenesis of Human Peripheral Blood Monocytes

doi: 10.3390/ijms20051002

Figure Lengend Snippet: TRAP5b activity in PBMC -M-CSF/RANKL (-CTRL), PBMC +M-CSF/RANKL (+CTRL), and PBMC +M-CSF/RANKL + DMAB (cDMAB), all cultured on titanium.

Article Snippet: Secretion of the tartrate-resistant acid phosphatase isoform 5b into the cell supernatant was measured using the TRAP5b ELISA kit (BlueGene Biotech, Shanghai, China) after 28 days of culture, following the manufacturer’s instructions.

Techniques: Activity Assay, Cell Culture