transit 2020 Search Results


transit 2020  (Mirus Bio)
97
Mirus Bio transit 2020
Transit 2020, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transit®-2020 transfection reagent  (Corning Life Sciences)
90
Corning Life Sciences transit®-2020 transfection reagent
Transit® 2020 Transfection Reagent, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transit®-2020 transfection reagent - by Bioz Stars, 2026-10
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treibhausgasneutralität in deutschland bis 2050: politikpapier zu rescue-studie  (Umweltbundesamt GmbH)
90
Umweltbundesamt GmbH treibhausgasneutralität in deutschland bis 2050: politikpapier zu rescue-studie
Treibhausgasneutralität In Deutschland Bis 2050: Politikpapier Zu Rescue Studie, supplied by Umweltbundesamt GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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treibhausgasneutralität in deutschland bis 2050: politikpapier zu rescue-studie - by Bioz Stars, 2026-10
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translt®-2020 transfection reagent  (BrainBits LLC)
90
BrainBits LLC translt®-2020 transfection reagent
Translt® 2020 Transfection Reagent, supplied by BrainBits LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
translt®-2020 transfection reagent - by Bioz Stars, 2026-10
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transit-2020  (US Biological Life Sciences)
90
US Biological Life Sciences transit-2020
Transit 2020, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/transit+2020/pmc04912856-105-4-19
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transit-2020 - by Bioz Stars, 2026-10
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transit-2020  (GENEFLOW LIMITED)
90
GENEFLOW LIMITED transit-2020
Transit 2020, supplied by GENEFLOW LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/transit+2020/pmc11222581-50-23-24
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transit- 2020  (PEQLAB)
90
PEQLAB transit- 2020
Transit 2020, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transit- 2020 - by Bioz Stars, 2026-10
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transit®-2020 transfection reagent  (GENEFLOW LIMITED)
90
GENEFLOW LIMITED transit®-2020 transfection reagent
Pi inhibits PP2A and induces an increase in Src phosphorylation. ( A ) Direct inhibition by Pi of PP2A catalytic activity after pulling-down endogenous PP2A-C from EA.hy926 ECs lysates and subjecting this to phosphatase catalytic activity assay in the presence of exogenous Pi at the stated concentration using PP2A phosphatase substrate (Millipore Ref 17-313). n = 3, *** p = 0.0002 versus control without Pi. ( B ) Representative immunoblot demonstrating Pi-induced inhibitory phosphorylation of PP2A in intact EA.hy926 cells. Effect of 1.5 h Pi-loading hyperphosphatemia on expression of PP2AC and p-PP2AC (Tyr307). ( C ) Densitometry analysis of ( B ), n = 3, * p = 0.037. ( D ) Representative p-Src immunoblots probed using anti-p-Src (Tyr419) antibody and ( E ) corresponding densitometry analysis of ( D ) n = 3, * p = 0.023. ( F ) Representative immunoblots and ( G ) quantitative analysis by densitometry of the effect of siRNA silencing of PP2A-C on Src phosphorylation during 1.5 h incubations of cells with 1 or 2.5 mM Pi. n = 3, * p = 0.0337. ( H ) Relative mRNA levels of PP2A-C in EA.hy926 cells transfected with scrambled siRNA and PP2A-C silencing siRNA for 1.5 h. After removal of the <t>transfection</t> medium and allowing an additional 24-h recovery period in growth medium, cultures were treated with 1 or 2.5 mM Pi, and RNA was extracted from the cells, reverse transcribed, and subjected to quantitative RT-PCR. n = 3, **** p < 0.0001.
Transit® 2020 Transfection Reagent, supplied by GENEFLOW LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/transit++2020+transfection+reagent/pmc07583816-98-14-19
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transit®-2020 transfection reagent - by Bioz Stars, 2026-10
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mirus transit-2020  (PEQLAB)
90
PEQLAB mirus transit-2020
Pi inhibits PP2A and induces an increase in Src phosphorylation. ( A ) Direct inhibition by Pi of PP2A catalytic activity after pulling-down endogenous PP2A-C from EA.hy926 ECs lysates and subjecting this to phosphatase catalytic activity assay in the presence of exogenous Pi at the stated concentration using PP2A phosphatase substrate (Millipore Ref 17-313). n = 3, *** p = 0.0002 versus control without Pi. ( B ) Representative immunoblot demonstrating Pi-induced inhibitory phosphorylation of PP2A in intact EA.hy926 cells. Effect of 1.5 h Pi-loading hyperphosphatemia on expression of PP2AC and p-PP2AC (Tyr307). ( C ) Densitometry analysis of ( B ), n = 3, * p = 0.037. ( D ) Representative p-Src immunoblots probed using anti-p-Src (Tyr419) antibody and ( E ) corresponding densitometry analysis of ( D ) n = 3, * p = 0.023. ( F ) Representative immunoblots and ( G ) quantitative analysis by densitometry of the effect of siRNA silencing of PP2A-C on Src phosphorylation during 1.5 h incubations of cells with 1 or 2.5 mM Pi. n = 3, * p = 0.0337. ( H ) Relative mRNA levels of PP2A-C in EA.hy926 cells transfected with scrambled siRNA and PP2A-C silencing siRNA for 1.5 h. After removal of the <t>transfection</t> medium and allowing an additional 24-h recovery period in growth medium, cultures were treated with 1 or 2.5 mM Pi, and RNA was extracted from the cells, reverse transcribed, and subjected to quantitative RT-PCR. n = 3, **** p < 0.0001.
Mirus Transit 2020, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/mirus+transit+2020/bio_rxiv__2023__09__11__557127-190-54-55
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mirus transit-2020 - by Bioz Stars, 2026-10
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transit 2020 kit transfection kit  (Muris Inc)
86
Muris Inc transit 2020 kit transfection kit
Pi inhibits PP2A and induces an increase in Src phosphorylation. ( A ) Direct inhibition by Pi of PP2A catalytic activity after pulling-down endogenous PP2A-C from EA.hy926 ECs lysates and subjecting this to phosphatase catalytic activity assay in the presence of exogenous Pi at the stated concentration using PP2A phosphatase substrate (Millipore Ref 17-313). n = 3, *** p = 0.0002 versus control without Pi. ( B ) Representative immunoblot demonstrating Pi-induced inhibitory phosphorylation of PP2A in intact EA.hy926 cells. Effect of 1.5 h Pi-loading hyperphosphatemia on expression of PP2AC and p-PP2AC (Tyr307). ( C ) Densitometry analysis of ( B ), n = 3, * p = 0.037. ( D ) Representative p-Src immunoblots probed using anti-p-Src (Tyr419) antibody and ( E ) corresponding densitometry analysis of ( D ) n = 3, * p = 0.023. ( F ) Representative immunoblots and ( G ) quantitative analysis by densitometry of the effect of siRNA silencing of PP2A-C on Src phosphorylation during 1.5 h incubations of cells with 1 or 2.5 mM Pi. n = 3, * p = 0.0337. ( H ) Relative mRNA levels of PP2A-C in EA.hy926 cells transfected with scrambled siRNA and PP2A-C silencing siRNA for 1.5 h. After removal of the <t>transfection</t> medium and allowing an additional 24-h recovery period in growth medium, cultures were treated with 1 or 2.5 mM Pi, and RNA was extracted from the cells, reverse transcribed, and subjected to quantitative RT-PCR. n = 3, **** p < 0.0001.
Transit 2020 Kit Transfection Kit, supplied by Muris Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/2020+kit+kit+transfection+transit/pulos_holmes_mia_christina__2020__non_canonical_roles_and_variants_of_core_translation_initiation_factors-696-12-11
Average 86 stars, based on 1 article reviews
transit 2020 kit transfection kit - by Bioz Stars, 2026-10
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transit 2020  (Merck & Co)
86
Merck & Co transit 2020
Pi inhibits PP2A and induces an increase in Src phosphorylation. ( A ) Direct inhibition by Pi of PP2A catalytic activity after pulling-down endogenous PP2A-C from EA.hy926 ECs lysates and subjecting this to phosphatase catalytic activity assay in the presence of exogenous Pi at the stated concentration using PP2A phosphatase substrate (Millipore Ref 17-313). n = 3, *** p = 0.0002 versus control without Pi. ( B ) Representative immunoblot demonstrating Pi-induced inhibitory phosphorylation of PP2A in intact EA.hy926 cells. Effect of 1.5 h Pi-loading hyperphosphatemia on expression of PP2AC and p-PP2AC (Tyr307). ( C ) Densitometry analysis of ( B ), n = 3, * p = 0.037. ( D ) Representative p-Src immunoblots probed using anti-p-Src (Tyr419) antibody and ( E ) corresponding densitometry analysis of ( D ) n = 3, * p = 0.023. ( F ) Representative immunoblots and ( G ) quantitative analysis by densitometry of the effect of siRNA silencing of PP2A-C on Src phosphorylation during 1.5 h incubations of cells with 1 or 2.5 mM Pi. n = 3, * p = 0.0337. ( H ) Relative mRNA levels of PP2A-C in EA.hy926 cells transfected with scrambled siRNA and PP2A-C silencing siRNA for 1.5 h. After removal of the <t>transfection</t> medium and allowing an additional 24-h recovery period in growth medium, cultures were treated with 1 or 2.5 mM Pi, and RNA was extracted from the cells, reverse transcribed, and subjected to quantitative RT-PCR. n = 3, **** p < 0.0001.
Transit 2020, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/2020+transit/bio_rxiv__2025__09__22__677701-122-5-9
Average 86 stars, based on 1 article reviews
transit 2020 - by Bioz Stars, 2026-10
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Image Search Results


Pi inhibits PP2A and induces an increase in Src phosphorylation. ( A ) Direct inhibition by Pi of PP2A catalytic activity after pulling-down endogenous PP2A-C from EA.hy926 ECs lysates and subjecting this to phosphatase catalytic activity assay in the presence of exogenous Pi at the stated concentration using PP2A phosphatase substrate (Millipore Ref 17-313). n = 3, *** p = 0.0002 versus control without Pi. ( B ) Representative immunoblot demonstrating Pi-induced inhibitory phosphorylation of PP2A in intact EA.hy926 cells. Effect of 1.5 h Pi-loading hyperphosphatemia on expression of PP2AC and p-PP2AC (Tyr307). ( C ) Densitometry analysis of ( B ), n = 3, * p = 0.037. ( D ) Representative p-Src immunoblots probed using anti-p-Src (Tyr419) antibody and ( E ) corresponding densitometry analysis of ( D ) n = 3, * p = 0.023. ( F ) Representative immunoblots and ( G ) quantitative analysis by densitometry of the effect of siRNA silencing of PP2A-C on Src phosphorylation during 1.5 h incubations of cells with 1 or 2.5 mM Pi. n = 3, * p = 0.0337. ( H ) Relative mRNA levels of PP2A-C in EA.hy926 cells transfected with scrambled siRNA and PP2A-C silencing siRNA for 1.5 h. After removal of the transfection medium and allowing an additional 24-h recovery period in growth medium, cultures were treated with 1 or 2.5 mM Pi, and RNA was extracted from the cells, reverse transcribed, and subjected to quantitative RT-PCR. n = 3, **** p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: Inorganic Phosphate (Pi) Signaling in Endothelial Cells: A Molecular Basis for Generation of Endothelial Microvesicles in Uraemic Cardiovascular Disease

doi: 10.3390/ijms21196993

Figure Lengend Snippet: Pi inhibits PP2A and induces an increase in Src phosphorylation. ( A ) Direct inhibition by Pi of PP2A catalytic activity after pulling-down endogenous PP2A-C from EA.hy926 ECs lysates and subjecting this to phosphatase catalytic activity assay in the presence of exogenous Pi at the stated concentration using PP2A phosphatase substrate (Millipore Ref 17-313). n = 3, *** p = 0.0002 versus control without Pi. ( B ) Representative immunoblot demonstrating Pi-induced inhibitory phosphorylation of PP2A in intact EA.hy926 cells. Effect of 1.5 h Pi-loading hyperphosphatemia on expression of PP2AC and p-PP2AC (Tyr307). ( C ) Densitometry analysis of ( B ), n = 3, * p = 0.037. ( D ) Representative p-Src immunoblots probed using anti-p-Src (Tyr419) antibody and ( E ) corresponding densitometry analysis of ( D ) n = 3, * p = 0.023. ( F ) Representative immunoblots and ( G ) quantitative analysis by densitometry of the effect of siRNA silencing of PP2A-C on Src phosphorylation during 1.5 h incubations of cells with 1 or 2.5 mM Pi. n = 3, * p = 0.0337. ( H ) Relative mRNA levels of PP2A-C in EA.hy926 cells transfected with scrambled siRNA and PP2A-C silencing siRNA for 1.5 h. After removal of the transfection medium and allowing an additional 24-h recovery period in growth medium, cultures were treated with 1 or 2.5 mM Pi, and RNA was extracted from the cells, reverse transcribed, and subjected to quantitative RT-PCR. n = 3, **** p < 0.0001.

Article Snippet: Plasmid transfection (1μg DNA per 35 mm culture) in EA.hy926 cells was performed using TransIT ® -2020 Transfection Reagent (Geneflow, Staffordshire, UK).

Techniques: Phospho-proteomics, Inhibition, Activity Assay, Concentration Assay, Control, Western Blot, Expressing, Transfection, Reverse Transcription, Quantitative RT-PCR