transient%20transfections Search Results


tcf lef activity assay wnt β catenin signaling activity  (BPS Bioscience)
93
BPS Bioscience tcf lef activity assay wnt β catenin signaling activity
Tcf Lef Activity Assay Wnt β Catenin Signaling Activity, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transfection collection nfκb transient pack  (BPS Bioscience)
88
BPS Bioscience transfection collection nfκb transient pack
Transfection Collection Nfκb Transient Pack, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transient transfection  (Promega)
90
Promega transient transfection
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Transient Transfection, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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membranes prepared from cos7 cells transiently transfected with human mutated 5ht2c receptor (ap-1)  (Tripos Inc)
90
Tripos Inc membranes prepared from cos7 cells transiently transfected with human mutated 5ht2c receptor (ap-1)
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Membranes Prepared From Cos7 Cells Transiently Transfected With Human Mutated 5ht2c Receptor (Ap 1), supplied by Tripos Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transient transfection  (Novartis)
90
Novartis transient transfection
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Transient Transfection, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transient transfections  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies transient transfections
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Transient Transfections, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transient transformation (i.e., transfection) of plants  (Fraunhofer USA)
90
Fraunhofer USA transient transformation (i.e., transfection) of plants
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Transient Transformation (I.E., Transfection) Of Plants, supplied by Fraunhofer USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard operating procedure for transient transfection  (LakePharma)
90
LakePharma standard operating procedure for transient transfection
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Standard Operating Procedure For Transient Transfection, supplied by LakePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atelogene kit for transient transfections  (KOKEN CO)
90
KOKEN CO atelogene kit for transient transfections
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Atelogene Kit For Transient Transfections, supplied by KOKEN CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transient transfection reagents attractene transfection reagent  (Qiagen)
90
Qiagen transient transfection reagents attractene transfection reagent
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Transient Transfection Reagents Attractene Transfection Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast-forward transfection protocol for adherent cells  (Qiagen)
90
Qiagen fast-forward transfection protocol for adherent cells
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Fast Forward Transfection Protocol For Adherent Cells, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectors for making the cells by transient or stable transfection  (Promega)
90
Promega vectors for making the cells by transient or stable transfection
Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, <t>transfection</t> of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.
Vectors For Making The Cells By Transient Or Stable Transfection, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, transfection of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.

Journal:

Article Title: The Glomerulosclerosis of Aging in Females

doi:

Figure Lengend Snippet: Increased NF-κB transcriptional activity in mesangial cells isolated from 28-month-old mice. Two separate mesangial cell lines were cultured from 5-month-old (5-month MC) or 28-month-old (28-month MC) female B6 mice. NF-κB transcriptional activity was measured by transfecting cells with a luciferase NF-κB reporter construct and a β-galactosidase plasmid. Basal NF-κB transcriptional activity in 5-month MC were arbitrarily defined as 1. Data shown are from six measurements of each of four independent experiments. A: Basal NF-κB transcriptional activity (base) was 2.6-fold higher in 28-month MC than in 5-month MC. The introduction of an exogenous IKK expression vector to 5-month MC or 28-month MC increased cell NF-κB transcriptional activity. In contrast, transfection of 5-month or 28-month MC with an IκB-α or dominant-negative IKK (dn) expression vector decreased cell NF-κB transcriptional activity. B: Increased sensitivity to TNF-α induced NF-κB activation in 28-month MC. Five ng/ml of TNF-α increased NF-κB transcriptional activity in 28-month MC but not in 5-month MC. An increase in TNF-α levels to 10 ng/ml was required to stimulate NF-κB activity in 5-month MC.

Article Snippet: Transfection and NF-κB Reporter Gene Assay Transient transfection was performed in mesangial cells from 5- and 28-month-old mice using Transfast according to the manufacturer’s protocol (Promega, Madison, WI).

Techniques: Activity Assay, Isolation, Cell Culture, Luciferase, Construct, Plasmid Preparation, Expressing, Transfection, Dominant Negative Mutation, Activation Assay