transfection Search Results


polyethyleneimine  (Kyfora Bio)
97
Kyfora Bio polyethyleneimine
Polyethyleneimine, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jetpei transfection reagent  (Sartorius AG)
95
Sartorius AG jetpei transfection reagent
Jetpei Transfection Reagent, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transporter 5 transfection reagent  (Kyfora Bio)
96
Kyfora Bio transporter 5 transfection reagent
Transporter 5 Transfection Reagent, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jetprime buffer  (Sartorius AG)
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Sartorius AG jetprime buffer
Jetprime Buffer, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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631312 calphos mammalian transfection kit  (TaKaRa)
97
TaKaRa 631312 calphos mammalian transfection kit
631312 Calphos Mammalian Transfection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transfections  (TaKaRa)
97
TaKaRa transfections
Transfections, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna transfection  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology sirna transfection
FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no <t>siRNA</t> (mock <t>transfection),</t> GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.
Sirna Transfection, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blu002a100uc pei max mw 40 000 polysciences  (Kyfora Bio)
98
Kyfora Bio blu002a100uc pei max mw 40 000 polysciences
FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no <t>siRNA</t> (mock <t>transfection),</t> GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.
Blu002a100uc Pei Max Mw 40 000 Polysciences, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pei transfection reagents  (MedChemExpress)
95
MedChemExpress pei transfection reagents
FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no <t>siRNA</t> (mock <t>transfection),</t> GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.
Pei Transfection Reagents, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna transfection  (TaKaRa)
95
TaKaRa sirna transfection
FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no <t>siRNA</t> (mock <t>transfection),</t> GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.
Sirna Transfection, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sitran 2 0 sirna transfection reagent  (OriGene)
95
OriGene sitran 2 0 sirna transfection reagent
FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no <t>siRNA</t> (mock <t>transfection),</t> GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.
Sitran 2 0 Sirna Transfection Reagent, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cells by interferin  (Sartorius AG)
94
Sartorius AG cells by interferin
FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no <t>siRNA</t> (mock <t>transfection),</t> GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.
Cells By Interferin, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no siRNA (mock transfection), GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.

Journal: Frontiers in cellular neuroscience

Article Title: CB 1 Cannabinoid Receptors Stimulate Gβγ-GRK2-Mediated FAK Phosphorylation at Tyrosine 925 to Regulate ERK Activation Involving Neuronal Focal Adhesions.

doi: 10.3389/fncel.2020.00176

Figure Lengend Snippet: FIGURE 5 | CB1-stimulated FAK phosphorylation at tyrosine 925 and ERK2 phosphorylation at tyrosine 204 are mediated by Gβγ and GRK2 in N18TG2 cells. (A–D) Cells were pretreated with the Gi/o inhibitor pertussis toxin (100 ng/mL, 20 h), Gβγ inhibitor gallein (10 µM, 15 min), or GRK2 inhibitor (1 µM, 15 min) before treatment with 0.01 µM WIN55212-2 (WIN) for 1 or 2 min (m) at 37◦C. Cell lysates were analyzed using western blots and representative blot images are shown. Data are reported as mean ± SEM of (B) % of vehicle-treated FAK 925 Tyr-P (normalized to total FAK levels) at the same time point, (C) % of vehicle-treated ERK2 204 Tyr-P (normalized to total ERK2 levels) at the same time point, and (D) % of basal/time 0 ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test [*p < 0.01, #p < 0.05 indicates significantly different from vehicle-treated (at the same time point); **p < 0.05 indicates significantly different from basal/time 0]. (E–H) Cells (2 × 105) were transfected with no siRNA (mock transfection), GRK2-specific siRNA (100 nM), or negative control siRNA (100 nM) before treatment with 0.01 µM WIN for 2 min at 37◦C. Immunoblot analysis was performed and data are reported as mean ± SEM of the % change over basal (G) FAK 925 Tyr-P (normalized to total FAK levels) and (H) ERK2 204 Tyr-P (normalized to total ERK2 levels). Significance was assessed using One Way ANOVA followed by Dunnett’s multiple comparisons posthoc test (*p < 0.01, #p < 0.05 indicates significantly different from GRK2 siRNA at the same time point). For each dataset, cells were cultured and experiments were completed on at least three separate occasions.

Article Snippet: N18TG2 cells were transfected with 100 nM GRK2-specific siRNA (mouse) or negative control siRNA-A using siRNA transfection reagent according to the manufacturer’s protocol (Santa Cruz Biotechnology).

Techniques: Phospho-proteomics, Western Blot, Transfection, Negative Control, Cell Culture