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Image Search Results
Journal: Avicenna Journal of Phytomedicine
Article Title: Carob (Ceratonia siliqua L.) fruit hydro-alcoholic extract alleviates reproductive toxicity of lead in male mice: Evidence on sperm parameters, sex hormones, oxidative stress biomarkers and expression of Nrf2 and iNOS
doi:
Figure Lengend Snippet: Primer specificity and cDNA synthesis products. A) Nrf2 (263 bp), B) and iNOS (170 bp) gene primer; Lane M: 100 bp DNA ladder, Lane 1, Control group; Lane 2, Pb group; Lane 3, Sham group; Lane 4, CFHAE 500 group; Lane 5, CFHAE 1000 group; Lane 6, Pb+CFHAE 500 group; Lane 7, Pb+CFHAE 1000 group
Article Snippet: ND-2000 spectrophotometer (Nanodrop Technologies, Thermo Fisher Scientific, USA) was used to determine concentration and purity of the total RNA and then the total RNA was stored at -70°C until cDNA synthesis. cDNA synthesis Using random hexamers primers (Primer+dNTP Mix+Nuclease-free water), first strand cDNA was synthesized from 10 μg of total RNA and
Techniques: