transconjugants Search Results


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  • 99
    Millipore transconjugants
    Phylogenetic tree showing the identified OTUs that contributed more than 0.01% in the transconjugant pools. (A) Showing <t>transconjugants</t> on D5. (B) Showing transconjugants on D75. The 16S rRNA gene sequence of Pseudomonas putida (donor strain) was shown in red letters and Nitrosotalea devanaterra (distant relative species to most of the transconjugants) was imported as the reference. The blue gradient circle at the periphery of the tree represents log of relative abundance of the OTU in the transconjugant pools.
    Transconjugants, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    ATCC transconjugants
    Phylogenetic tree showing the identified OTUs that contributed more than 0.01% in the transconjugant pools. (A) Showing <t>transconjugants</t> on D5. (B) Showing transconjugants on D75. The 16S rRNA gene sequence of Pseudomonas putida (donor strain) was shown in red letters and Nitrosotalea devanaterra (distant relative species to most of the transconjugants) was imported as the reference. The blue gradient circle at the periphery of the tree represents log of relative abundance of the OTU in the transconjugant pools.
    Transconjugants, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    INCF transconjugants
    Plasmid profiles for strain 33676 and its transformant or transconjugant derivatives. Strain 33676 carries three plasmids of about 150, 120 and 5 kb, corresponding to IncA/C, IncF and ColE1-like plasmids, respectively (lanes 1 and 7). E.coli DH5α derivatives were obtained by conjugation or transformation. Transformants were obtained with chloramphenicol (Tf-cm, lane 2), ceftriaxone (Tf-CRO, lane 3), and tetracycline (Tf-tc, lane 4); <t>transconjugants</t> were obtained with tetracycline (Tc-tc, lane 5), and ceftriaxone (Tc-CRO, lane 6)
    Transconjugants, supplied by INCF, used in various techniques. Bioz Stars score: 99/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher transconjugants
    Conjugation experiment showing the growth of <t>transconjugants</t> confirming the ability of transferring genetic material carrying drug resistance genes to competent
    Transconjugants, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Pro-Lab nonhemolytic transconjugants
    Southern hybridization analysis of Hin dIII restriction digests of genomic DNA probed with tetM . (A) Hemolytic wild-type T18P (lane 1) does not hybridize with the tetM probe. <t>Nonhemolytic</t> <t>transconjugants</t> SB1-4, SB2-9, SB30-2, SB1-9, SB5-9, SB6-9, SB7-9, SB1-1, and SB8-2 (lanes 2 to 10, respectively) all contain at least one copy of Tn 916 and hybridize with the tetM probe. Isolates in lanes 2, 7, and 9 possess more than one Tn 916 insertion. All lanes possess two bands hybridizing with the tetM probe corresponding to approximately 6.5 and 14 kb. The Tn 916 donor strain CG110 (lane 11) contains several copies of Tn 916 . (B) Hemolytic wild-type MGAS166s (lane 1) does not hybridize with the tetM probe. The nonhemolytic transconjugants SBNH1, SBNH3, SBNH4, SBNH5, SBNH6, SBNH7, and SBNH8 (lanes 2 to 8, respectively) all possess at least one copy of Tn 916 . Isolates in lanes 3, 4, 7, and 8 possess more than one Tn 916 insertion. Isolates in all lanes possess two bands of similar size, approximately 14 and 7.5 kb. The migration of molecular size standards (1-kb ladder) is indicated on the left.
    Nonhemolytic Transconjugants, supplied by Pro-Lab, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    INCF transconjugants tr59 incf
    Southern hybridization analysis of Hin dIII restriction digests of genomic DNA probed with tetM . (A) Hemolytic wild-type T18P (lane 1) does not hybridize with the tetM probe. <t>Nonhemolytic</t> <t>transconjugants</t> SB1-4, SB2-9, SB30-2, SB1-9, SB5-9, SB6-9, SB7-9, SB1-1, and SB8-2 (lanes 2 to 10, respectively) all contain at least one copy of Tn 916 and hybridize with the tetM probe. Isolates in lanes 2, 7, and 9 possess more than one Tn 916 insertion. All lanes possess two bands hybridizing with the tetM probe corresponding to approximately 6.5 and 14 kb. The Tn 916 donor strain CG110 (lane 11) contains several copies of Tn 916 . (B) Hemolytic wild-type MGAS166s (lane 1) does not hybridize with the tetM probe. The nonhemolytic transconjugants SBNH1, SBNH3, SBNH4, SBNH5, SBNH6, SBNH7, and SBNH8 (lanes 2 to 8, respectively) all possess at least one copy of Tn 916 . Isolates in lanes 3, 4, 7, and 8 possess more than one Tn 916 insertion. Isolates in all lanes possess two bands of similar size, approximately 14 and 7.5 kb. The migration of molecular size standards (1-kb ladder) is indicated on the left.
    Transconjugants Tr59 Incf, supplied by INCF, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Agencourt Bioscience Corporation transconjugant
    Southern hybridization analysis of Hin dIII restriction digests of genomic DNA probed with tetM . (A) Hemolytic wild-type T18P (lane 1) does not hybridize with the tetM probe. <t>Nonhemolytic</t> <t>transconjugants</t> SB1-4, SB2-9, SB30-2, SB1-9, SB5-9, SB6-9, SB7-9, SB1-1, and SB8-2 (lanes 2 to 10, respectively) all contain at least one copy of Tn 916 and hybridize with the tetM probe. Isolates in lanes 2, 7, and 9 possess more than one Tn 916 insertion. All lanes possess two bands hybridizing with the tetM probe corresponding to approximately 6.5 and 14 kb. The Tn 916 donor strain CG110 (lane 11) contains several copies of Tn 916 . (B) Hemolytic wild-type MGAS166s (lane 1) does not hybridize with the tetM probe. The nonhemolytic transconjugants SBNH1, SBNH3, SBNH4, SBNH5, SBNH6, SBNH7, and SBNH8 (lanes 2 to 8, respectively) all possess at least one copy of Tn 916 . Isolates in lanes 3, 4, 7, and 8 possess more than one Tn 916 insertion. Isolates in all lanes possess two bands of similar size, approximately 14 and 7.5 kb. The migration of molecular size standards (1-kb ladder) is indicated on the left.
    Transconjugant, supplied by Agencourt Bioscience Corporation, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Becton Dickinson transconjugants
    Antimicrobial susceptibility profiles done on the 22 conjugative isolates (parents) and their <t>transconjugants</t> for the antimicrobial agents, ceftazidime (CAZ), cefotaxime (CTX), cefpodoxime (CPD) and ciprofloxacin (CIP) .
    Transconjugants, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 98/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Clinical and Laboratory Standards Institute transconjugants
    A heat-map showing the comparison of ESBL-positive E. coli donors and the resultant <t>transconjugants,</t> characterized on the basis of their plasmid profiles; ESBL-markers identified by PCR; antimicrobial resistance profile; and plasmid replicon type(s). The MIC for cefotaxime in each pairwise combination is also shown along with the fold increase in this value. (T) in the first column typifies the transconjugant. Black and white squares denote the presence and absence, respectively of a particular feature. The symbol “–” signifies no fold-change in the MIC for the transconjugant. Antimicrobial compounds are abbreviated as follows: AM, ampicillin; AMC, amoxicillin–clavulanic acid; C, chloramphenicol; CPD, cefpodoxime; CIP, ciprofloxacin; CTX, cefotaxime; KF, cephalothin; NA, nalidixic acid; S, streptomycin; SXT, trimethoprim-sulfamethoxazole; TE, tetracycline; W, trimethoprim.
    Transconjugants, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Difco transconjugants
    Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the <t>transconjugants</t> that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p
    Transconjugants, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc transconjugants
    Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the <t>transconjugants</t> that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p
    Transconjugants, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche transconjugants
    Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the <t>transconjugants</t> that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p
    Transconjugants, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Pacific Biosciences transconjugants
    Crossover regions. (A) Cartoon representation of crossover regions in all individual <t>transconjugants.</t> The area of the crossover is measured from the last SNV corresponding to the donor strain, C68, to the first SNV corresponding to the recipient strain, D344RRF. Transconjugants are organized by groups with shared left crossover regions. Notice the additional crossover regions in TC-D and TC-M (light gray). The amount of chromosomal DNA integrated in addition to Tn 5382 is measured from the left or right end of the element up to the last SNV between D344RRF and each transconjugant. The amount of integrated DNA is shown in kilobases. (B) Plot of GC and AT contents of C68 (donor) and D344RRF (recipient) in the chromosomal region where crossovers occurred. Blue, GC content; green, AT content. The high-GC-content area in C68 corresponding to Tn 5382 carrying vanB is highlighted between red lines.
    Transconjugants, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    INCF transconjugant nm4 carried incf
    Crossover regions. (A) Cartoon representation of crossover regions in all individual <t>transconjugants.</t> The area of the crossover is measured from the last SNV corresponding to the donor strain, C68, to the first SNV corresponding to the recipient strain, D344RRF. Transconjugants are organized by groups with shared left crossover regions. Notice the additional crossover regions in TC-D and TC-M (light gray). The amount of chromosomal DNA integrated in addition to Tn 5382 is measured from the left or right end of the element up to the last SNV between D344RRF and each transconjugant. The amount of integrated DNA is shown in kilobases. (B) Plot of GC and AT contents of C68 (donor) and D344RRF (recipient) in the chromosomal region where crossovers occurred. Blue, GC content; green, AT content. The high-GC-content area in C68 corresponding to Tn 5382 carrying vanB is highlighted between red lines.
    Transconjugant Nm4 Carried Incf, supplied by INCF, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Eiken Chemical transconjugant selective macconkey agar
    Crossover regions. (A) Cartoon representation of crossover regions in all individual <t>transconjugants.</t> The area of the crossover is measured from the last SNV corresponding to the donor strain, C68, to the first SNV corresponding to the recipient strain, D344RRF. Transconjugants are organized by groups with shared left crossover regions. Notice the additional crossover regions in TC-D and TC-M (light gray). The amount of chromosomal DNA integrated in addition to Tn 5382 is measured from the left or right end of the element up to the last SNV between D344RRF and each transconjugant. The amount of integrated DNA is shown in kilobases. (B) Plot of GC and AT contents of C68 (donor) and D344RRF (recipient) in the chromosomal region where crossovers occurred. Blue, GC content; green, AT content. The high-GC-content area in C68 corresponding to Tn 5382 carrying vanB is highlighted between red lines.
    Transconjugant Selective Macconkey Agar, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher transconjugant px1
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Transconjugant Px1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    INCF transconjugants transformants
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Transconjugants Transformants, supplied by INCF, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    INCF tr65 incf transconjugant
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Tr65 Incf Transconjugant, supplied by INCF, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    INCF tr102 incf transconjugants
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Tr102 Incf Transconjugants, supplied by INCF, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    INCF tet resistant transconjugants
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Tet Resistant Transconjugants, supplied by INCF, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Illumina Inc transconjugant l lactis nizo3921
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Transconjugant L Lactis Nizo3921, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC salmonella species transconjugants
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Salmonella Species Transconjugants, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC transconjugant atcc 11845
    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 <t>pX1</t> (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C <t>transconjugant</t> plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.
    Transconjugant Atcc 11845, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC transconjugate strain
    Expression of the TCS in strains W83 and ATCC 33277. A. Quantitative RT-PCR of the HK and RR genes. Results were obtained from five independent cultures of strains W83 and ATCC 33277 grown to OD550 nm 0.5 using 1 mg RNA from each sample. B. Western blot of RR production in strain W83 (lane 1) and ATC C33277 (lane 2). C. Western blot of PGN_0753 response regulator production in ATCC 33277 parent (lane1) and <t>transconjugate</t> TR719 (lane 2). Each lane contains 10 µg of total protein. Blots were probed with rabbit anti-PG0720 primary- and HRP-conjugated goat anti-rabbit secondary antibodies.
    Transconjugate Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    GATC Biotech p fluorescens pf0 1 transconjugants
    Conversion of β -valine to β -valinyl-CoA by cell-free extracts of cells grown on medium supplemented with β -valine and ammonium sulfate as nitrogen sources. Relative β -valinyl-CoA levels in the samples were analyzed after 0, 2, and 4 h of incubation using HPLC, where β -valinyl-CoA levels in SBV1 cell extracts after 2 h incubation was set at 100 %. Pf-WT β -valine growth-deficient strain Pseudomonas <t>fluorescens</t> <t>Pf0-1,</t> Pf-Comp P. fluorescens Pf0-1 containing the bvaA gene on the complementing plasmid p4-D1, SBV1 β -valine-degrading organism described in this study
    P Fluorescens Pf0 1 Transconjugants, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    INCF transconjugants harboring incf plasmids
    Conversion of β -valine to β -valinyl-CoA by cell-free extracts of cells grown on medium supplemented with β -valine and ammonium sulfate as nitrogen sources. Relative β -valinyl-CoA levels in the samples were analyzed after 0, 2, and 4 h of incubation using HPLC, where β -valinyl-CoA levels in SBV1 cell extracts after 2 h incubation was set at 100 %. Pf-WT β -valine growth-deficient strain Pseudomonas <t>fluorescens</t> <t>Pf0-1,</t> Pf-Comp P. fluorescens Pf0-1 containing the bvaA gene on the complementing plasmid p4-D1, SBV1 β -valine-degrading organism described in this study
    Transconjugants Harboring Incf Plasmids, supplied by INCF, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC atcc 700685 transconjugants
    Conversion of β -valine to β -valinyl-CoA by cell-free extracts of cells grown on medium supplemented with β -valine and ammonium sulfate as nitrogen sources. Relative β -valinyl-CoA levels in the samples were analyzed after 0, 2, and 4 h of incubation using HPLC, where β -valinyl-CoA levels in SBV1 cell extracts after 2 h incubation was set at 100 %. Pf-WT β -valine growth-deficient strain Pseudomonas <t>fluorescens</t> <t>Pf0-1,</t> Pf-Comp P. fluorescens Pf0-1 containing the bvaA gene on the complementing plasmid p4-D1, SBV1 β -valine-degrading organism described in this study
    Atcc 700685 Transconjugants, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC m haemolytica atcc 33396 transconjugants
    Identification of tRNA-leu UUG as the putative attB insertion site of ICE Mh1 -like elements and predicted host range. (A) Schematic for ICE orientation and random PCR mapping of ICE Mh1 PM22 junctions in 18 Mannheimia <t>haemolytica</t> and 18 Pasteurella multocida <t>transconjugants.</t> ICE Mh1 PM22 insertions in every transconjugant were identical and aligned to a specific tRNA-leu in the genomes of P. multocida strain 36950 and M. haemolytica M42548. (B) Sequence logo of left and right MUSCLE-aligned junctions identified in 41 Pasteurellaceae harboring ICE Mh1 -like sequences. The left and right ends of ICE Mh1 -like variants contain a conserved direct repeat (DR; 5′-GATTCAAAATC-3′) attL conserves the tRNA TψC loop’s imperfect palindrome (5′-CGGTTCGAGTCCG-3′). The attL and attR sites are designated with respect to ICE Pmu1 in Michael et al. (2011b) . (C) Neighbor-joining tree of all tRNA-leu from 41 Pasteurellaceae harboring ICE Mh1 -like sequences. All ICE Mh1 -like insertions were associated with tRNA-leu UUG . (D) Predicted structure of tRNA-leu UUG showing presumptive ICE Mh1 attB attachment site (also encoding the tRNA anticodon) and palindrome. (E) Frequencies of tRNA-leu codon types in 41 Pasteurellaceae harboring ICE Mh1 -like sequences. (F) Predicted host range of ICE Mh1 -like elements based on tRNA-leu UUG alignment by BLAST and strict (100% identity) conservation of direct repeat and palindrome. Colors are arbitrary.
    M Haemolytica Atcc 33396 Transconjugants, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    GATC Biotech pai positive e faecalis transconjugant strain og1rfxuw3114 t 12
    Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the E. <t>faecalis</t> <t>PAI</t> from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the <t>transconjugant</t> strains compared to the corresponding recipient (p
    Pai Positive E Faecalis Transconjugant Strain Og1rfxuw3114 T 12, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC p aeruginosa atcc 27853 transconjugants
    In vitro antibacterial kinetics of AN3365. (A) The viability of E. coli ATCC 25922 over 24 h in MHB treated with AN3365 at 4-fold and 10-fold its MIC and the control ciprofloxacin at 4-fold its MIC. (B) The viability of P. <t>aeruginosa</t> <t>ATCC</t> 27853 over 24
    P Aeruginosa Atcc 27853 Transconjugants, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Phylogenetic tree showing the identified OTUs that contributed more than 0.01% in the transconjugant pools. (A) Showing transconjugants on D5. (B) Showing transconjugants on D75. The 16S rRNA gene sequence of Pseudomonas putida (donor strain) was shown in red letters and Nitrosotalea devanaterra (distant relative species to most of the transconjugants) was imported as the reference. The blue gradient circle at the periphery of the tree represents log of relative abundance of the OTU in the transconjugant pools.

    Journal: Frontiers in Microbiology

    Article Title: Fate of Antibiotic Resistant Pseudomonas putida and Broad Host Range Plasmid in Natural Soil Microcosms

    doi: 10.3389/fmicb.2019.00194

    Figure Lengend Snippet: Phylogenetic tree showing the identified OTUs that contributed more than 0.01% in the transconjugant pools. (A) Showing transconjugants on D5. (B) Showing transconjugants on D75. The 16S rRNA gene sequence of Pseudomonas putida (donor strain) was shown in red letters and Nitrosotalea devanaterra (distant relative species to most of the transconjugants) was imported as the reference. The blue gradient circle at the periphery of the tree represents log of relative abundance of the OTU in the transconjugant pools.

    Article Snippet: Bacterial cells including indigenous soil bacteria (no fluorescence), transconjugants (green fluorescence) and donor strains (red fluorescence) were scaled by FlowSight Imaging Flow Cytometer (Amnis Millipore, United States).

    Techniques: Sequencing

    Plasmid profiles for strain 33676 and its transformant or transconjugant derivatives. Strain 33676 carries three plasmids of about 150, 120 and 5 kb, corresponding to IncA/C, IncF and ColE1-like plasmids, respectively (lanes 1 and 7). E.coli DH5α derivatives were obtained by conjugation or transformation. Transformants were obtained with chloramphenicol (Tf-cm, lane 2), ceftriaxone (Tf-CRO, lane 3), and tetracycline (Tf-tc, lane 4); transconjugants were obtained with tetracycline (Tc-tc, lane 5), and ceftriaxone (Tc-CRO, lane 6)

    Journal: BMC Microbiology

    Article Title: A multi-drug resistant Salmonella Typhimurium ST213 human-invasive strain (33676) containing the blaCMY-2 gene on an IncF plasmid is attenuated for virulence in BALB/c mice

    doi: 10.1186/s12866-016-0633-7

    Figure Lengend Snippet: Plasmid profiles for strain 33676 and its transformant or transconjugant derivatives. Strain 33676 carries three plasmids of about 150, 120 and 5 kb, corresponding to IncA/C, IncF and ColE1-like plasmids, respectively (lanes 1 and 7). E.coli DH5α derivatives were obtained by conjugation or transformation. Transformants were obtained with chloramphenicol (Tf-cm, lane 2), ceftriaxone (Tf-CRO, lane 3), and tetracycline (Tf-tc, lane 4); transconjugants were obtained with tetracycline (Tc-tc, lane 5), and ceftriaxone (Tc-CRO, lane 6)

    Article Snippet: Transconjugants were positive for the amplification with the IncA/C primers; however, they were also positive for amplification with the IncF primers, indicating that both plasmids were transferred.

    Techniques: Plasmid Preparation, Conjugation Assay, Transformation Assay

    Conjugation experiment showing the growth of transconjugants confirming the ability of transferring genetic material carrying drug resistance genes to competent

    Journal: Bioinformation

    Article Title: Studies on New Delhi Metallo-Beta-Lactamse-1 producing Acinetobacter baumannii isolated from donor swab in a tertiary eye care centre, India and structural analysis of its antibiotic binding interactions

    doi: 10.6026/97320630008445

    Figure Lengend Snippet: Conjugation experiment showing the growth of transconjugants confirming the ability of transferring genetic material carrying drug resistance genes to competent

    Article Snippet: Mixtures were incubated without shaking at 37°C for 18 h. Transconjugants were selected by their ability to resist streptomycin 65 µg/ml (Oxoid), tetracycline 100 µg/ml (Merck, India) and cefotaxime 2 µg/ml (Himedia, India) incorporated in LB agar [ , ].

    Techniques: Conjugation Assay, Transferring

    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 pX1 (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C transconjugant plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.

    Journal: BMC Microbiology

    Article Title: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2 gene through genetic re-arrangements with an IncX1 plasmid

    doi: 10.1186/1471-2180-13-264

    Figure Lengend Snippet: Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 pX1 (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C transconjugant plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.

    Article Snippet: The E. coli DH5α transformants carrying wild-type or transconjugant pA/C were digested with 15 U of Pst I (Invitrogen) at 37°C for 6 hours, whereas DH5α transformants carrying wild-type or transconjugant pX1 were simultaneously digested with 10 U of Bam HI and Nco I (Fermentas) at 37°C for 3 hours.

    Techniques: Generated

    Examples of pA/C transconjugants recovered in SO1 pSTV ::Km and DH5α. Panel A) shows the plasmid profiles of four different transconjugants in SO1 marked within dotted rectangles. The donor YU39 pA/C and the recipient SO1pSTV ::Km strains are in the first and last lanes, respectively. Within each dotted rectangle, in the first lane are the SO1 transconjugants; in the second and third lanes the DH5α transformants for the pA/C and pSTV of each transconjugant are shown. Panel B) displays examples of Pst I restriction profiles of pA/C transconjugants of SO1 and DH5α compared wit h wild-type YU39 pA/C (DH5α-pA/C).

    Journal: BMC Microbiology

    Article Title: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2 gene through genetic re-arrangements with an IncX1 plasmid

    doi: 10.1186/1471-2180-13-264

    Figure Lengend Snippet: Examples of pA/C transconjugants recovered in SO1 pSTV ::Km and DH5α. Panel A) shows the plasmid profiles of four different transconjugants in SO1 marked within dotted rectangles. The donor YU39 pA/C and the recipient SO1pSTV ::Km strains are in the first and last lanes, respectively. Within each dotted rectangle, in the first lane are the SO1 transconjugants; in the second and third lanes the DH5α transformants for the pA/C and pSTV of each transconjugant are shown. Panel B) displays examples of Pst I restriction profiles of pA/C transconjugants of SO1 and DH5α compared wit h wild-type YU39 pA/C (DH5α-pA/C).

    Article Snippet: The E. coli DH5α transformants carrying wild-type or transconjugant pA/C were digested with 15 U of Pst I (Invitrogen) at 37°C for 6 hours, whereas DH5α transformants carrying wild-type or transconjugant pX1 were simultaneously digested with 10 U of Bam HI and Nco I (Fermentas) at 37°C for 3 hours.

    Techniques: Plasmid Preparation

    Schematic diagram of the CMY regions of Typhimurium strain YU39 and pX1 :: CMY transconjugants. Panel A) shows a schematic diagram of the CMY region in the pA/C plasmid of strain YU39 [ 5 ]. Panel B) depicts a large CMY region inserted into the intergenic region between 046 and 047 genes for IC2 transconjugant. Panel C) shows a short CMY region inserted into stbE gene for IIIC10 transconjugant. The PCR amplifications designed to assess the extension of the CMY regions are indicated by double arrowheads under the diagrams. The PCRs used to determine the pX1 CMY junctions are indicated by bars with circles.

    Journal: BMC Microbiology

    Article Title: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2 gene through genetic re-arrangements with an IncX1 plasmid

    doi: 10.1186/1471-2180-13-264

    Figure Lengend Snippet: Schematic diagram of the CMY regions of Typhimurium strain YU39 and pX1 :: CMY transconjugants. Panel A) shows a schematic diagram of the CMY region in the pA/C plasmid of strain YU39 [ 5 ]. Panel B) depicts a large CMY region inserted into the intergenic region between 046 and 047 genes for IC2 transconjugant. Panel C) shows a short CMY region inserted into stbE gene for IIIC10 transconjugant. The PCR amplifications designed to assess the extension of the CMY regions are indicated by double arrowheads under the diagrams. The PCRs used to determine the pX1 CMY junctions are indicated by bars with circles.

    Article Snippet: The E. coli DH5α transformants carrying wild-type or transconjugant pA/C were digested with 15 U of Pst I (Invitrogen) at 37°C for 6 hours, whereas DH5α transformants carrying wild-type or transconjugant pX1 were simultaneously digested with 10 U of Bam HI and Nco I (Fermentas) at 37°C for 3 hours.

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    Southern hybridization analysis of Hin dIII restriction digests of genomic DNA probed with tetM . (A) Hemolytic wild-type T18P (lane 1) does not hybridize with the tetM probe. Nonhemolytic transconjugants SB1-4, SB2-9, SB30-2, SB1-9, SB5-9, SB6-9, SB7-9, SB1-1, and SB8-2 (lanes 2 to 10, respectively) all contain at least one copy of Tn 916 and hybridize with the tetM probe. Isolates in lanes 2, 7, and 9 possess more than one Tn 916 insertion. All lanes possess two bands hybridizing with the tetM probe corresponding to approximately 6.5 and 14 kb. The Tn 916 donor strain CG110 (lane 11) contains several copies of Tn 916 . (B) Hemolytic wild-type MGAS166s (lane 1) does not hybridize with the tetM probe. The nonhemolytic transconjugants SBNH1, SBNH3, SBNH4, SBNH5, SBNH6, SBNH7, and SBNH8 (lanes 2 to 8, respectively) all possess at least one copy of Tn 916 . Isolates in lanes 3, 4, 7, and 8 possess more than one Tn 916 insertion. Isolates in all lanes possess two bands of similar size, approximately 14 and 7.5 kb. The migration of molecular size standards (1-kb ladder) is indicated on the left.

    Journal: Infection and Immunity

    Article Title: Reduced Virulence of Group A Streptococcal Tn916 Mutants That Do Not Produce Streptolysin S

    doi:

    Figure Lengend Snippet: Southern hybridization analysis of Hin dIII restriction digests of genomic DNA probed with tetM . (A) Hemolytic wild-type T18P (lane 1) does not hybridize with the tetM probe. Nonhemolytic transconjugants SB1-4, SB2-9, SB30-2, SB1-9, SB5-9, SB6-9, SB7-9, SB1-1, and SB8-2 (lanes 2 to 10, respectively) all contain at least one copy of Tn 916 and hybridize with the tetM probe. Isolates in lanes 2, 7, and 9 possess more than one Tn 916 insertion. All lanes possess two bands hybridizing with the tetM probe corresponding to approximately 6.5 and 14 kb. The Tn 916 donor strain CG110 (lane 11) contains several copies of Tn 916 . (B) Hemolytic wild-type MGAS166s (lane 1) does not hybridize with the tetM probe. The nonhemolytic transconjugants SBNH1, SBNH3, SBNH4, SBNH5, SBNH6, SBNH7, and SBNH8 (lanes 2 to 8, respectively) all possess at least one copy of Tn 916 . Isolates in lanes 3, 4, 7, and 8 possess more than one Tn 916 insertion. Isolates in all lanes possess two bands of similar size, approximately 14 and 7.5 kb. The migration of molecular size standards (1-kb ladder) is indicated on the left.

    Article Snippet: M typing of nonhemolytic transconjugants confirmed that M protein was produced, and both SB30-2 and SBNH5 had the same M-protein phenotypes as their M18 and M1 parent strains, respectively.

    Techniques: Hybridization, Migration

    Antimicrobial susceptibility profiles done on the 22 conjugative isolates (parents) and their transconjugants for the antimicrobial agents, ceftazidime (CAZ), cefotaxime (CTX), cefpodoxime (CPD) and ciprofloxacin (CIP) .

    Journal: Annals of Clinical Microbiology and Antimicrobials

    Article Title: Frequency of conjugative transfer of plasmid-encoded ISEcp1 - blaCTX-M-15 and aac(6')-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon - role of transferases

    doi: 10.1186/1476-0711-9-19

    Figure Lengend Snippet: Antimicrobial susceptibility profiles done on the 22 conjugative isolates (parents) and their transconjugants for the antimicrobial agents, ceftazidime (CAZ), cefotaxime (CTX), cefpodoxime (CPD) and ciprofloxacin (CIP) .

    Article Snippet: The transconjugants were selected using Mueller-Hinton II (Becton, Dickinson and company-BBL® ) agar plates containing 100 mg/l sodium azide plus 2 mg/l cefotaxime[ ].

    Techniques:

    A heat-map showing the comparison of ESBL-positive E. coli donors and the resultant transconjugants, characterized on the basis of their plasmid profiles; ESBL-markers identified by PCR; antimicrobial resistance profile; and plasmid replicon type(s). The MIC for cefotaxime in each pairwise combination is also shown along with the fold increase in this value. (T) in the first column typifies the transconjugant. Black and white squares denote the presence and absence, respectively of a particular feature. The symbol “–” signifies no fold-change in the MIC for the transconjugant. Antimicrobial compounds are abbreviated as follows: AM, ampicillin; AMC, amoxicillin–clavulanic acid; C, chloramphenicol; CPD, cefpodoxime; CIP, ciprofloxacin; CTX, cefotaxime; KF, cephalothin; NA, nalidixic acid; S, streptomycin; SXT, trimethoprim-sulfamethoxazole; TE, tetracycline; W, trimethoprim.

    Journal: Frontiers in Microbiology

    Article Title: Molecular characterization of blaESBL-harboring conjugative plasmids identified in multi-drug resistant Escherichia coli isolated from food-producing animals and healthy humans

    doi: 10.3389/fmicb.2013.00188

    Figure Lengend Snippet: A heat-map showing the comparison of ESBL-positive E. coli donors and the resultant transconjugants, characterized on the basis of their plasmid profiles; ESBL-markers identified by PCR; antimicrobial resistance profile; and plasmid replicon type(s). The MIC for cefotaxime in each pairwise combination is also shown along with the fold increase in this value. (T) in the first column typifies the transconjugant. Black and white squares denote the presence and absence, respectively of a particular feature. The symbol “–” signifies no fold-change in the MIC for the transconjugant. Antimicrobial compounds are abbreviated as follows: AM, ampicillin; AMC, amoxicillin–clavulanic acid; C, chloramphenicol; CPD, cefpodoxime; CIP, ciprofloxacin; CTX, cefotaxime; KF, cephalothin; NA, nalidixic acid; S, streptomycin; SXT, trimethoprim-sulfamethoxazole; TE, tetracycline; W, trimethoprim.

    Article Snippet: ANTIMICROBIAL SUSCEPTIBILITY TESTING Donor strains and the corresponding transconjugants were tested for their susceptibility to a panel of antimicrobial compounds by disk diffusion, following recommendations of the Clinical and Laboratory Standards Institute (CLSI).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p

    Article Snippet: Transconjugants were selected on Brain Hearth Infusion (BHI) agar (Difco Labs., Detroit, USA) containing the selective marker for the donor (Erythromycin 10mg/L) and those for the recipient (rifampicin 30mg/L and fusidic acid 20mg/L).

    Techniques: Expressing, End-sequence Profiling, Flow Cytometry, Cytometry, FACS

    Comparison of in vitro biofilm formation. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the plasmid pLG2 and E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the significant increase in Biofilm formation of PAI positive E. faecalis transconjugant. The mean values of three different experiments are shown. Error bars denote standard deviation. Experiments were performed four times, each time in triplicate.

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Comparison of in vitro biofilm formation. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the plasmid pLG2 and E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the significant increase in Biofilm formation of PAI positive E. faecalis transconjugant. The mean values of three different experiments are shown. Error bars denote standard deviation. Experiments were performed four times, each time in triplicate.

    Article Snippet: Transconjugants were selected on Brain Hearth Infusion (BHI) agar (Difco Labs., Detroit, USA) containing the selective marker for the donor (Erythromycin 10mg/L) and those for the recipient (rifampicin 30mg/L and fusidic acid 20mg/L).

    Techniques: In Vitro, Plasmid Preparation, Standard Deviation

    A. S1-nuclease analysis showing transfer of a ca. 66 kb conjugative plasmid into both transconjugants. B. Sma I restriction analysis and corresponding Southern hybridization using an esp probe. None of the plasmid bands hybridized to an esp probe (not shown). M, Marker S. aureus 8325 Sma I -digested; D, Donor UW3114; Efm-R, E. faecium recipient 64/3; Efm T-10, E. faecium transconjugant 64/3xUW3114 T-10; Efs-R, E. faecalis recipient OG1RF; Efs T-12, E. faecalis transconjugant OG1RFxUW3114 T-12. White arrows indicate the fragment size shift due to PAI acquisition in E. faecalis ( ca. 206 kb larger) and E. faecium ( ca. 193 kb larger).

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: A. S1-nuclease analysis showing transfer of a ca. 66 kb conjugative plasmid into both transconjugants. B. Sma I restriction analysis and corresponding Southern hybridization using an esp probe. None of the plasmid bands hybridized to an esp probe (not shown). M, Marker S. aureus 8325 Sma I -digested; D, Donor UW3114; Efm-R, E. faecium recipient 64/3; Efm T-10, E. faecium transconjugant 64/3xUW3114 T-10; Efs-R, E. faecalis recipient OG1RF; Efs T-12, E. faecalis transconjugant OG1RFxUW3114 T-12. White arrows indicate the fragment size shift due to PAI acquisition in E. faecalis ( ca. 206 kb larger) and E. faecium ( ca. 193 kb larger).

    Article Snippet: Transconjugants were selected on Brain Hearth Infusion (BHI) agar (Difco Labs., Detroit, USA) containing the selective marker for the donor (Erythromycin 10mg/L) and those for the recipient (rifampicin 30mg/L and fusidic acid 20mg/L).

    Techniques: Plasmid Preparation, Hybridization, End-sequence Profiling, Marker

    Comparative in vitro growth of E. faecium (left) and E. faecalis (right) recipient and transconjugant strains. 64/3 and OG1RF are the recipient strains. 64/3 T-1 * (64/3xUW3114 T-1) and OG1RF T-1 * (OG1RFxUW3114 T-1) are transconjugants that acquired the erm (B) plasmid pLG2 but lack the PAI. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that carry pLG2 and the E. faecalis PAI; comparisson of their growth to that of the corresponding recipient strains indicated no significant differences both in E. faecium (p = 0.9909) as in E. faecalis (p = 0.9329). Error bars denote standard deviation. Each experiment was repeated three times.

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Comparative in vitro growth of E. faecium (left) and E. faecalis (right) recipient and transconjugant strains. 64/3 and OG1RF are the recipient strains. 64/3 T-1 * (64/3xUW3114 T-1) and OG1RF T-1 * (OG1RFxUW3114 T-1) are transconjugants that acquired the erm (B) plasmid pLG2 but lack the PAI. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that carry pLG2 and the E. faecalis PAI; comparisson of their growth to that of the corresponding recipient strains indicated no significant differences both in E. faecium (p = 0.9909) as in E. faecalis (p = 0.9329). Error bars denote standard deviation. Each experiment was repeated three times.

    Article Snippet: Transconjugants were selected on Brain Hearth Infusion (BHI) agar (Difco Labs., Detroit, USA) containing the selective marker for the donor (Erythromycin 10mg/L) and those for the recipient (rifampicin 30mg/L and fusidic acid 20mg/L).

    Techniques: In Vitro, Plasmid Preparation, Standard Deviation

    Esp expression shown by transmission electron microscopy of cells negatively stained and labelled with inmunogold (15 nm) using anti-Esp antiserum. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3xUW3114 T-10 and OG1RFxUW3114 T-12 are the transconjugants that acquired the plasmid pLG2 and the E. faecalis PAI from strain UW3114 (See also Table 2 ). Bar lenght = 200 nm.

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Esp expression shown by transmission electron microscopy of cells negatively stained and labelled with inmunogold (15 nm) using anti-Esp antiserum. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3xUW3114 T-10 and OG1RFxUW3114 T-12 are the transconjugants that acquired the plasmid pLG2 and the E. faecalis PAI from strain UW3114 (See also Table 2 ). Bar lenght = 200 nm.

    Article Snippet: Transconjugants were selected on Brain Hearth Infusion (BHI) agar (Difco Labs., Detroit, USA) containing the selective marker for the donor (Erythromycin 10mg/L) and those for the recipient (rifampicin 30mg/L and fusidic acid 20mg/L).

    Techniques: End-sequence Profiling, Expressing, Transmission Assay, Electron Microscopy, Staining, Plasmid Preparation

    Bacterial counts in the blood (left) and kidneys (right) 24 h after (above) intraperitoneal and (below) intravenous injection of eight female BALB/c mice (6–8-week-old) with E. faecalis strains. OG1RF is the recipient strain, OG1RF T-1* (OG1RFxUW3114 T-1) acquired the plasmid pLG2 but lacks the PAI, OG1RF T-12 (OG1RFxUW3114 T-12) is the transconjugant that acquired pLG2 and the E. faecalis PAI (See also Table 2 ). Data represent the individual bacterial counts and the media. No significant differences were observed between the recipient and transconjugants in any of the settings (p > 0.50). The inocula used for the bacteraemia model were OG1RF: 8.5× , OG1RFxUW3114 T-1: 5.5× , OG1RFxUW3114 T-12: 5.2× . The inocula used for the peritonitis model were OG1RF: 4.4× , OG1RFxUW3114 T-1: 5.3× , OG1RFxUW3114 T-12: 5.7× .

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Bacterial counts in the blood (left) and kidneys (right) 24 h after (above) intraperitoneal and (below) intravenous injection of eight female BALB/c mice (6–8-week-old) with E. faecalis strains. OG1RF is the recipient strain, OG1RF T-1* (OG1RFxUW3114 T-1) acquired the plasmid pLG2 but lacks the PAI, OG1RF T-12 (OG1RFxUW3114 T-12) is the transconjugant that acquired pLG2 and the E. faecalis PAI (See also Table 2 ). Data represent the individual bacterial counts and the media. No significant differences were observed between the recipient and transconjugants in any of the settings (p > 0.50). The inocula used for the bacteraemia model were OG1RF: 8.5× , OG1RFxUW3114 T-1: 5.5× , OG1RFxUW3114 T-12: 5.2× . The inocula used for the peritonitis model were OG1RF: 4.4× , OG1RFxUW3114 T-1: 5.3× , OG1RFxUW3114 T-12: 5.7× .

    Article Snippet: Transconjugants were selected on Brain Hearth Infusion (BHI) agar (Difco Labs., Detroit, USA) containing the selective marker for the donor (Erythromycin 10mg/L) and those for the recipient (rifampicin 30mg/L and fusidic acid 20mg/L).

    Techniques: Injection, Mouse Assay, Plasmid Preparation

    Crossover regions. (A) Cartoon representation of crossover regions in all individual transconjugants. The area of the crossover is measured from the last SNV corresponding to the donor strain, C68, to the first SNV corresponding to the recipient strain, D344RRF. Transconjugants are organized by groups with shared left crossover regions. Notice the additional crossover regions in TC-D and TC-M (light gray). The amount of chromosomal DNA integrated in addition to Tn 5382 is measured from the left or right end of the element up to the last SNV between D344RRF and each transconjugant. The amount of integrated DNA is shown in kilobases. (B) Plot of GC and AT contents of C68 (donor) and D344RRF (recipient) in the chromosomal region where crossovers occurred. Blue, GC content; green, AT content. The high-GC-content area in C68 corresponding to Tn 5382 carrying vanB is highlighted between red lines.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Homologous Recombination within Large Chromosomal Regions Facilitates Acquisition of β-Lactam and Vancomycin Resistance in Enterococcus faecium

    doi: 10.1128/AAC.00488-16

    Figure Lengend Snippet: Crossover regions. (A) Cartoon representation of crossover regions in all individual transconjugants. The area of the crossover is measured from the last SNV corresponding to the donor strain, C68, to the first SNV corresponding to the recipient strain, D344RRF. Transconjugants are organized by groups with shared left crossover regions. Notice the additional crossover regions in TC-D and TC-M (light gray). The amount of chromosomal DNA integrated in addition to Tn 5382 is measured from the left or right end of the element up to the last SNV between D344RRF and each transconjugant. The amount of integrated DNA is shown in kilobases. (B) Plot of GC and AT contents of C68 (donor) and D344RRF (recipient) in the chromosomal region where crossovers occurred. Blue, GC content; green, AT content. The high-GC-content area in C68 corresponding to Tn 5382 carrying vanB is highlighted between red lines.

    Article Snippet: After the identification of the canonical pLRM23 sequence using the PacBio assemblies, we searched for the presence of genes of plasmid origin in the transconjugants by mapping the Fastq reads from D344RRF and the transconjugants versus the PacBio assembly of C68 (see Table S5C in the supplemental material) and then by comparing the regions that were unique to C68 and the transconjugants but not to D344RRF.

    Techniques:

    Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 pX1 (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C transconjugant plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.

    Journal: BMC Microbiology

    Article Title: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2 gene through genetic re-arrangements with an IncX1 plasmid

    doi: 10.1186/1471-2180-13-264

    Figure Lengend Snippet: Representative restriction profiles for pA/C transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 pX1 (DH5α-pX1) and pA/C (DH5α-pA/C) and representative pA/C transconjugant plasmids. The nomenclature of the transconjugants is shown in Table 4 . White stars at the right side of the bands indicate positive hybridizations signals with the pX1 probe.

    Article Snippet: The E. coli DH5α transformants carrying wild-type or transconjugant pA/C were digested with 15 U of Pst I (Invitrogen) at 37°C for 6 hours, whereas DH5α transformants carrying wild-type or transconjugant pX1 were simultaneously digested with 10 U of Bam HI and Nco I (Fermentas) at 37°C for 3 hours.

    Techniques: Generated

    Representative restriction profiles for pX1 + CMY transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 pX1 (DH5α-pX1) and representative transconjugant plasmids. The nomenclature of the transconjugants is shown in Table 3 .

    Journal: BMC Microbiology

    Article Title: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2 gene through genetic re-arrangements with an IncX1 plasmid

    doi: 10.1186/1471-2180-13-264

    Figure Lengend Snippet: Representative restriction profiles for pX1 + CMY transconjugants. Double digestions with BamH I- Nco I were generated for the wild-type YU39 pX1 (DH5α-pX1) and representative transconjugant plasmids. The nomenclature of the transconjugants is shown in Table 3 .

    Article Snippet: The E. coli DH5α transformants carrying wild-type or transconjugant pA/C were digested with 15 U of Pst I (Invitrogen) at 37°C for 6 hours, whereas DH5α transformants carrying wild-type or transconjugant pX1 were simultaneously digested with 10 U of Bam HI and Nco I (Fermentas) at 37°C for 3 hours.

    Techniques: Generated

    Schematic diagram of the CMY regions of Typhimurium strain YU39 and pX1 :: CMY transconjugants. Panel A) shows a schematic diagram of the CMY region in the pA/C plasmid of strain YU39 [ 5 ]. Panel B) depicts a large CMY region inserted into the intergenic region between 046 and 047 genes for IC2 transconjugant. Panel C) shows a short CMY region inserted into stbE gene for IIIC10 transconjugant. The PCR amplifications designed to assess the extension of the CMY regions are indicated by double arrowheads under the diagrams. The PCRs used to determine the pX1 CMY junctions are indicated by bars with circles.

    Journal: BMC Microbiology

    Article Title: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2 gene through genetic re-arrangements with an IncX1 plasmid

    doi: 10.1186/1471-2180-13-264

    Figure Lengend Snippet: Schematic diagram of the CMY regions of Typhimurium strain YU39 and pX1 :: CMY transconjugants. Panel A) shows a schematic diagram of the CMY region in the pA/C plasmid of strain YU39 [ 5 ]. Panel B) depicts a large CMY region inserted into the intergenic region between 046 and 047 genes for IC2 transconjugant. Panel C) shows a short CMY region inserted into stbE gene for IIIC10 transconjugant. The PCR amplifications designed to assess the extension of the CMY regions are indicated by double arrowheads under the diagrams. The PCRs used to determine the pX1 CMY junctions are indicated by bars with circles.

    Article Snippet: The E. coli DH5α transformants carrying wild-type or transconjugant pA/C were digested with 15 U of Pst I (Invitrogen) at 37°C for 6 hours, whereas DH5α transformants carrying wild-type or transconjugant pX1 were simultaneously digested with 10 U of Bam HI and Nco I (Fermentas) at 37°C for 3 hours.

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    Expression of the TCS in strains W83 and ATCC 33277. A. Quantitative RT-PCR of the HK and RR genes. Results were obtained from five independent cultures of strains W83 and ATCC 33277 grown to OD550 nm 0.5 using 1 mg RNA from each sample. B. Western blot of RR production in strain W83 (lane 1) and ATC C33277 (lane 2). C. Western blot of PGN_0753 response regulator production in ATCC 33277 parent (lane1) and transconjugate TR719 (lane 2). Each lane contains 10 µg of total protein. Blots were probed with rabbit anti-PG0720 primary- and HRP-conjugated goat anti-rabbit secondary antibodies.

    Journal: PLoS ONE

    Article Title: A Two-Component System Regulates Hemin Acquisition in Porphyromonas gingivalis

    doi: 10.1371/journal.pone.0073351

    Figure Lengend Snippet: Expression of the TCS in strains W83 and ATCC 33277. A. Quantitative RT-PCR of the HK and RR genes. Results were obtained from five independent cultures of strains W83 and ATCC 33277 grown to OD550 nm 0.5 using 1 mg RNA from each sample. B. Western blot of RR production in strain W83 (lane 1) and ATC C33277 (lane 2). C. Western blot of PGN_0753 response regulator production in ATCC 33277 parent (lane1) and transconjugate TR719 (lane 2). Each lane contains 10 µg of total protein. Blots were probed with rabbit anti-PG0720 primary- and HRP-conjugated goat anti-rabbit secondary antibodies.

    Article Snippet: We constructed a transconjugate strain (TR719) of ATCC 33277 that carried the functional HK (PG0719) from W83 on pT-COW and tested whether production of RR PGN_0753 was restored.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    The functional HK from strain W83 restores growth defects of ATCC 33277. A. Growth of strains under hemin-deplete and replete conditions (0, 0.001, and 0.5 µg/ml, respectively: n = 3). B. Expression of RR PGN_0753 in ATCC 33277/pTCOW and TR719 in hemin-deplete, -limited, and -replete conditions measured by QRT-PCR. C. Expression of genes involved in iron/hemin transport in ATCC 33277/pTCOW and transconjugant TR719 grown under hemin- depleted and replete conditions. Data were obtained by QRT-PCR. PGN_1681: ATP-transporter ATP-binding protein.

    Journal: PLoS ONE

    Article Title: A Two-Component System Regulates Hemin Acquisition in Porphyromonas gingivalis

    doi: 10.1371/journal.pone.0073351

    Figure Lengend Snippet: The functional HK from strain W83 restores growth defects of ATCC 33277. A. Growth of strains under hemin-deplete and replete conditions (0, 0.001, and 0.5 µg/ml, respectively: n = 3). B. Expression of RR PGN_0753 in ATCC 33277/pTCOW and TR719 in hemin-deplete, -limited, and -replete conditions measured by QRT-PCR. C. Expression of genes involved in iron/hemin transport in ATCC 33277/pTCOW and transconjugant TR719 grown under hemin- depleted and replete conditions. Data were obtained by QRT-PCR. PGN_1681: ATP-transporter ATP-binding protein.

    Article Snippet: Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719).

    Techniques: Functional Assay, Expressing, Quantitative RT-PCR, Binding Assay

    Venn diagram depicting numbers of genes up- or -down regulated in ATCC 33277 during growth in hemin- depleted, -limited and replete media. Gene expression was quantified by microarray and fold changes were calculated by dividing values for transconjugant TR719 by those for ATCC 33277 containing pTCOW empty vector. For up-regulated genes the cutoff was at least a two-fold increase and for down regulated a decrease of at least 0.62.

    Journal: PLoS ONE

    Article Title: A Two-Component System Regulates Hemin Acquisition in Porphyromonas gingivalis

    doi: 10.1371/journal.pone.0073351

    Figure Lengend Snippet: Venn diagram depicting numbers of genes up- or -down regulated in ATCC 33277 during growth in hemin- depleted, -limited and replete media. Gene expression was quantified by microarray and fold changes were calculated by dividing values for transconjugant TR719 by those for ATCC 33277 containing pTCOW empty vector. For up-regulated genes the cutoff was at least a two-fold increase and for down regulated a decrease of at least 0.62.

    Article Snippet: Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719).

    Techniques: Expressing, Microarray, Plasmid Preparation

    Conversion of β -valine to β -valinyl-CoA by cell-free extracts of cells grown on medium supplemented with β -valine and ammonium sulfate as nitrogen sources. Relative β -valinyl-CoA levels in the samples were analyzed after 0, 2, and 4 h of incubation using HPLC, where β -valinyl-CoA levels in SBV1 cell extracts after 2 h incubation was set at 100 %. Pf-WT β -valine growth-deficient strain Pseudomonas fluorescens Pf0-1, Pf-Comp P. fluorescens Pf0-1 containing the bvaA gene on the complementing plasmid p4-D1, SBV1 β -valine-degrading organism described in this study

    Journal: Applied Microbiology and Biotechnology

    Article Title: Metabolism of β-valine via a CoA-dependent ammonia lyase pathway

    doi: 10.1007/s00253-015-6551-z

    Figure Lengend Snippet: Conversion of β -valine to β -valinyl-CoA by cell-free extracts of cells grown on medium supplemented with β -valine and ammonium sulfate as nitrogen sources. Relative β -valinyl-CoA levels in the samples were analyzed after 0, 2, and 4 h of incubation using HPLC, where β -valinyl-CoA levels in SBV1 cell extracts after 2 h incubation was set at 100 %. Pf-WT β -valine growth-deficient strain Pseudomonas fluorescens Pf0-1, Pf-Comp P. fluorescens Pf0-1 containing the bvaA gene on the complementing plasmid p4-D1, SBV1 β -valine-degrading organism described in this study

    Article Snippet: Sequencing of pLAFR3 inserts from specific P. fluorescens Pf0-1 transconjugants was done at GATC Biotech, using various primers.

    Techniques: Incubation, High Performance Liquid Chromatography, Plasmid Preparation

    Identification of tRNA-leu UUG as the putative attB insertion site of ICE Mh1 -like elements and predicted host range. (A) Schematic for ICE orientation and random PCR mapping of ICE Mh1 PM22 junctions in 18 Mannheimia haemolytica and 18 Pasteurella multocida transconjugants. ICE Mh1 PM22 insertions in every transconjugant were identical and aligned to a specific tRNA-leu in the genomes of P. multocida strain 36950 and M. haemolytica M42548. (B) Sequence logo of left and right MUSCLE-aligned junctions identified in 41 Pasteurellaceae harboring ICE Mh1 -like sequences. The left and right ends of ICE Mh1 -like variants contain a conserved direct repeat (DR; 5′-GATTCAAAATC-3′) attL conserves the tRNA TψC loop’s imperfect palindrome (5′-CGGTTCGAGTCCG-3′). The attL and attR sites are designated with respect to ICE Pmu1 in Michael et al. (2011b) . (C) Neighbor-joining tree of all tRNA-leu from 41 Pasteurellaceae harboring ICE Mh1 -like sequences. All ICE Mh1 -like insertions were associated with tRNA-leu UUG . (D) Predicted structure of tRNA-leu UUG showing presumptive ICE Mh1 attB attachment site (also encoding the tRNA anticodon) and palindrome. (E) Frequencies of tRNA-leu codon types in 41 Pasteurellaceae harboring ICE Mh1 -like sequences. (F) Predicted host range of ICE Mh1 -like elements based on tRNA-leu UUG alignment by BLAST and strict (100% identity) conservation of direct repeat and palindrome. Colors are arbitrary.

    Journal: Frontiers in Microbiology

    Article Title: Emerging Variants of the Integrative and Conjugant Element ICEMh1 in Livestock Pathogens: Structural Insights, Potential Host Range, and Implications for Bacterial Fitness and Antimicrobial Therapy

    doi: 10.3389/fmicb.2019.02608

    Figure Lengend Snippet: Identification of tRNA-leu UUG as the putative attB insertion site of ICE Mh1 -like elements and predicted host range. (A) Schematic for ICE orientation and random PCR mapping of ICE Mh1 PM22 junctions in 18 Mannheimia haemolytica and 18 Pasteurella multocida transconjugants. ICE Mh1 PM22 insertions in every transconjugant were identical and aligned to a specific tRNA-leu in the genomes of P. multocida strain 36950 and M. haemolytica M42548. (B) Sequence logo of left and right MUSCLE-aligned junctions identified in 41 Pasteurellaceae harboring ICE Mh1 -like sequences. The left and right ends of ICE Mh1 -like variants contain a conserved direct repeat (DR; 5′-GATTCAAAATC-3′) attL conserves the tRNA TψC loop’s imperfect palindrome (5′-CGGTTCGAGTCCG-3′). The attL and attR sites are designated with respect to ICE Pmu1 in Michael et al. (2011b) . (C) Neighbor-joining tree of all tRNA-leu from 41 Pasteurellaceae harboring ICE Mh1 -like sequences. All ICE Mh1 -like insertions were associated with tRNA-leu UUG . (D) Predicted structure of tRNA-leu UUG showing presumptive ICE Mh1 attB attachment site (also encoding the tRNA anticodon) and palindrome. (E) Frequencies of tRNA-leu codon types in 41 Pasteurellaceae harboring ICE Mh1 -like sequences. (F) Predicted host range of ICE Mh1 -like elements based on tRNA-leu UUG alignment by BLAST and strict (100% identity) conservation of direct repeat and palindrome. Colors are arbitrary.

    Article Snippet: MIC Determination and Checkerboard Assay Antimicrobial Synergy Screening Minimum inhibitory concentrations (MICs) were determined for the RifR and isogenic ICEMh1 PM22 P. multocida CCUG 17976 and M. haemolytica ATCC 33396 transconjugants according to the CLSI approved standard M07 - Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically ( ).

    Techniques: Polymerase Chain Reaction, Sequencing

    ICE Mh1 PM22 fitness costs, addiction, and consequences for antimicrobial resistance in transconjugants. (A) Growth curves of P. multocida CCUG 17976 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (B) Growth curves of M. haemolytica ATCC 33396 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (C) Luciferase-based competition index from co-cultures of ICE Mh1 PM22 transconjugants and E. coli DH5α harboring the luciferase expression plasmid pAKlux2. Mean of 4 biological replicates with SEM; t-test, ∗ P ≤ 0.05. (D) Long-term repeated passage of ICE Mh1 PM22 transconjugants. Transconjugants were grown in MH (no drug) or MH + 0.5 MIC (subinhibitory; MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396 WT) for 150 days and plated on media with or without selective concentrations of indicated antimicrobials (i.e., 0.5 MIC for non-susceptible isogenic transconjugants). Mean of 3 biological replicates with SEM. (E) Upper panel: growth curves of co-cultures of E. coli DH5α pAKlux2 and E. coli DH5α, P. multocida CCUG 17976 ICE Mh1 PM22 , or M. haemolytica ATCC 33396 ICE Mh1 PM22 in subinhibitory (0.5 MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396) concentrations of oxytetracycline (left) or spectinomycin (right). Mean of 3 biological replicates with SEM. Lower panel: detection of E. coli luciferase production in co-cultures (as above) with either oxytetracycline (left) or spectinomycin (right). (F) Schematic of checkerboard synergy assay for drug interaction screening.

    Journal: Frontiers in Microbiology

    Article Title: Emerging Variants of the Integrative and Conjugant Element ICEMh1 in Livestock Pathogens: Structural Insights, Potential Host Range, and Implications for Bacterial Fitness and Antimicrobial Therapy

    doi: 10.3389/fmicb.2019.02608

    Figure Lengend Snippet: ICE Mh1 PM22 fitness costs, addiction, and consequences for antimicrobial resistance in transconjugants. (A) Growth curves of P. multocida CCUG 17976 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (B) Growth curves of M. haemolytica ATCC 33396 (spontaneous rifampin-resistant mutant) and isogenic ICE Mh1 PM22 transconjugant. Mean of 4 biological replicates with SEM. (C) Luciferase-based competition index from co-cultures of ICE Mh1 PM22 transconjugants and E. coli DH5α harboring the luciferase expression plasmid pAKlux2. Mean of 4 biological replicates with SEM; t-test, ∗ P ≤ 0.05. (D) Long-term repeated passage of ICE Mh1 PM22 transconjugants. Transconjugants were grown in MH (no drug) or MH + 0.5 MIC (subinhibitory; MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396 WT) for 150 days and plated on media with or without selective concentrations of indicated antimicrobials (i.e., 0.5 MIC for non-susceptible isogenic transconjugants). Mean of 3 biological replicates with SEM. (E) Upper panel: growth curves of co-cultures of E. coli DH5α pAKlux2 and E. coli DH5α, P. multocida CCUG 17976 ICE Mh1 PM22 , or M. haemolytica ATCC 33396 ICE Mh1 PM22 in subinhibitory (0.5 MIC for susceptible P. multocida CCUG 17976 or M. haemolytica ATCC 33396) concentrations of oxytetracycline (left) or spectinomycin (right). Mean of 3 biological replicates with SEM. Lower panel: detection of E. coli luciferase production in co-cultures (as above) with either oxytetracycline (left) or spectinomycin (right). (F) Schematic of checkerboard synergy assay for drug interaction screening.

    Article Snippet: MIC Determination and Checkerboard Assay Antimicrobial Synergy Screening Minimum inhibitory concentrations (MICs) were determined for the RifR and isogenic ICEMh1 PM22 P. multocida CCUG 17976 and M. haemolytica ATCC 33396 transconjugants according to the CLSI approved standard M07 - Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically ( ).

    Techniques: Mutagenesis, Luciferase, Expressing, Plasmid Preparation

    Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Differential expression of cell wall-associated Esp at 37°C and 21°C measured by flow cytometry (FACS) of anti-Esp labelled cells. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the enhancement of esp expression in the transconjugant strains compared to the corresponding recipient (p

    Article Snippet: 5 g) from PAI-positive E. faecalis transconjugant strain OG1RFxUW3114 T-12 was subjected to 454 sequencing at GATC Biotech (Konstanz, Germany).

    Techniques: Expressing, End-sequence Profiling, Flow Cytometry, Cytometry, FACS

    Comparison of in vitro biofilm formation. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the plasmid pLG2 and E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the significant increase in Biofilm formation of PAI positive E. faecalis transconjugant. The mean values of three different experiments are shown. Error bars denote standard deviation. Experiments were performed four times, each time in triplicate.

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Comparison of in vitro biofilm formation. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that acquired the plasmid pLG2 and E. faecalis PAI from strain UW3114 (See also Table 2 ). Notice the significant increase in Biofilm formation of PAI positive E. faecalis transconjugant. The mean values of three different experiments are shown. Error bars denote standard deviation. Experiments were performed four times, each time in triplicate.

    Article Snippet: 5 g) from PAI-positive E. faecalis transconjugant strain OG1RFxUW3114 T-12 was subjected to 454 sequencing at GATC Biotech (Konstanz, Germany).

    Techniques: In Vitro, Plasmid Preparation, Standard Deviation

    A. S1-nuclease analysis showing transfer of a ca. 66 kb conjugative plasmid into both transconjugants. B. Sma I restriction analysis and corresponding Southern hybridization using an esp probe. None of the plasmid bands hybridized to an esp probe (not shown). M, Marker S. aureus 8325 Sma I -digested; D, Donor UW3114; Efm-R, E. faecium recipient 64/3; Efm T-10, E. faecium transconjugant 64/3xUW3114 T-10; Efs-R, E. faecalis recipient OG1RF; Efs T-12, E. faecalis transconjugant OG1RFxUW3114 T-12. White arrows indicate the fragment size shift due to PAI acquisition in E. faecalis ( ca. 206 kb larger) and E. faecium ( ca. 193 kb larger).

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: A. S1-nuclease analysis showing transfer of a ca. 66 kb conjugative plasmid into both transconjugants. B. Sma I restriction analysis and corresponding Southern hybridization using an esp probe. None of the plasmid bands hybridized to an esp probe (not shown). M, Marker S. aureus 8325 Sma I -digested; D, Donor UW3114; Efm-R, E. faecium recipient 64/3; Efm T-10, E. faecium transconjugant 64/3xUW3114 T-10; Efs-R, E. faecalis recipient OG1RF; Efs T-12, E. faecalis transconjugant OG1RFxUW3114 T-12. White arrows indicate the fragment size shift due to PAI acquisition in E. faecalis ( ca. 206 kb larger) and E. faecium ( ca. 193 kb larger).

    Article Snippet: 5 g) from PAI-positive E. faecalis transconjugant strain OG1RFxUW3114 T-12 was subjected to 454 sequencing at GATC Biotech (Konstanz, Germany).

    Techniques: Plasmid Preparation, Hybridization, End-sequence Profiling, Marker

    Comparative in vitro growth of E. faecium (left) and E. faecalis (right) recipient and transconjugant strains. 64/3 and OG1RF are the recipient strains. 64/3 T-1 * (64/3xUW3114 T-1) and OG1RF T-1 * (OG1RFxUW3114 T-1) are transconjugants that acquired the erm (B) plasmid pLG2 but lack the PAI. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that carry pLG2 and the E. faecalis PAI; comparisson of their growth to that of the corresponding recipient strains indicated no significant differences both in E. faecium (p = 0.9909) as in E. faecalis (p = 0.9329). Error bars denote standard deviation. Each experiment was repeated three times.

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Comparative in vitro growth of E. faecium (left) and E. faecalis (right) recipient and transconjugant strains. 64/3 and OG1RF are the recipient strains. 64/3 T-1 * (64/3xUW3114 T-1) and OG1RF T-1 * (OG1RFxUW3114 T-1) are transconjugants that acquired the erm (B) plasmid pLG2 but lack the PAI. Strains 64/3 T-10 (64/3xUW3114 T-10) and OG1RF T-12 (OG1RFxUW3114 T-12) are the transconjugants that carry pLG2 and the E. faecalis PAI; comparisson of their growth to that of the corresponding recipient strains indicated no significant differences both in E. faecium (p = 0.9909) as in E. faecalis (p = 0.9329). Error bars denote standard deviation. Each experiment was repeated three times.

    Article Snippet: 5 g) from PAI-positive E. faecalis transconjugant strain OG1RFxUW3114 T-12 was subjected to 454 sequencing at GATC Biotech (Konstanz, Germany).

    Techniques: In Vitro, Plasmid Preparation, Standard Deviation

    Esp expression shown by transmission electron microscopy of cells negatively stained and labelled with inmunogold (15 nm) using anti-Esp antiserum. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3xUW3114 T-10 and OG1RFxUW3114 T-12 are the transconjugants that acquired the plasmid pLG2 and the E. faecalis PAI from strain UW3114 (See also Table 2 ). Bar lenght = 200 nm.

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Esp expression shown by transmission electron microscopy of cells negatively stained and labelled with inmunogold (15 nm) using anti-Esp antiserum. Strains UW3114 (donor) and MMH594 (reference) are shown for comparison. 64/3 and OG1RF are the recipient strains. Strains 64/3xUW3114 T-10 and OG1RFxUW3114 T-12 are the transconjugants that acquired the plasmid pLG2 and the E. faecalis PAI from strain UW3114 (See also Table 2 ). Bar lenght = 200 nm.

    Article Snippet: 5 g) from PAI-positive E. faecalis transconjugant strain OG1RFxUW3114 T-12 was subjected to 454 sequencing at GATC Biotech (Konstanz, Germany).

    Techniques: End-sequence Profiling, Expressing, Transmission Assay, Electron Microscopy, Staining, Plasmid Preparation

    Bacterial counts in the blood (left) and kidneys (right) 24 h after (above) intraperitoneal and (below) intravenous injection of eight female BALB/c mice (6–8-week-old) with E. faecalis strains. OG1RF is the recipient strain, OG1RF T-1* (OG1RFxUW3114 T-1) acquired the plasmid pLG2 but lacks the PAI, OG1RF T-12 (OG1RFxUW3114 T-12) is the transconjugant that acquired pLG2 and the E. faecalis PAI (See also Table 2 ). Data represent the individual bacterial counts and the media. No significant differences were observed between the recipient and transconjugants in any of the settings (p > 0.50). The inocula used for the bacteraemia model were OG1RF: 8.5× , OG1RFxUW3114 T-1: 5.5× , OG1RFxUW3114 T-12: 5.2× . The inocula used for the peritonitis model were OG1RF: 4.4× , OG1RFxUW3114 T-1: 5.3× , OG1RFxUW3114 T-12: 5.7× .

    Journal: PLoS ONE

    Article Title: Intra- and Interspecies Genomic Transfer of the Enterococcus faecalis Pathogenicity Island

    doi: 10.1371/journal.pone.0016720

    Figure Lengend Snippet: Bacterial counts in the blood (left) and kidneys (right) 24 h after (above) intraperitoneal and (below) intravenous injection of eight female BALB/c mice (6–8-week-old) with E. faecalis strains. OG1RF is the recipient strain, OG1RF T-1* (OG1RFxUW3114 T-1) acquired the plasmid pLG2 but lacks the PAI, OG1RF T-12 (OG1RFxUW3114 T-12) is the transconjugant that acquired pLG2 and the E. faecalis PAI (See also Table 2 ). Data represent the individual bacterial counts and the media. No significant differences were observed between the recipient and transconjugants in any of the settings (p > 0.50). The inocula used for the bacteraemia model were OG1RF: 8.5× , OG1RFxUW3114 T-1: 5.5× , OG1RFxUW3114 T-12: 5.2× . The inocula used for the peritonitis model were OG1RF: 4.4× , OG1RFxUW3114 T-1: 5.3× , OG1RFxUW3114 T-12: 5.7× .

    Article Snippet: 5 g) from PAI-positive E. faecalis transconjugant strain OG1RFxUW3114 T-12 was subjected to 454 sequencing at GATC Biotech (Konstanz, Germany).

    Techniques: Injection, Mouse Assay, Plasmid Preparation

    In vitro antibacterial kinetics of AN3365. (A) The viability of E. coli ATCC 25922 over 24 h in MHB treated with AN3365 at 4-fold and 10-fold its MIC and the control ciprofloxacin at 4-fold its MIC. (B) The viability of P. aeruginosa ATCC 27853 over 24

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Discovery of a Novel Class of Boron-Based Antibacterials with Activity against Gram-Negative Bacteria

    doi: 10.1128/AAC.02058-12

    Figure Lengend Snippet: In vitro antibacterial kinetics of AN3365. (A) The viability of E. coli ATCC 25922 over 24 h in MHB treated with AN3365 at 4-fold and 10-fold its MIC and the control ciprofloxacin at 4-fold its MIC. (B) The viability of P. aeruginosa ATCC 27853 over 24

    Article Snippet: P. aeruginosa ATCC 27853 transconjugants were selected by their gentamicin and chloramphemicol resistance, and their sensitivity to 5% (wt/vol) sucrose was confirmed.

    Techniques: In Vitro