tract Search Results


rna binding protein multiple splice rbpms polypeptide  (ProSci Incorporated)
90
ProSci Incorporated rna binding protein multiple splice rbpms polypeptide
Figure 1. Expression of L-, P/Q-, N-type Ca channels in the proximal retinal. Ca channel a1 subunits co-express with the ganglion cell antibody <t>RBPMS</t> in vertical sections showing the inner plexiform layer (IPL), the ganglion cell layer (GCL) and the nerve fiber layer (NFL). (Top row) Double immunostaining with a1C subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). RGC axons (arrowhead) as well as putative Mu¨ller and neuronal cell processes in the IPL showed immunostaining. Control preadsorption of a1C antibody with the immunization peptide showed no staining. (Second row) Double immunostaining with a1D subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Numerous processes in the IPL as well as the RGC axons throughout the NFL (arrowhead) were also labelled. Control preadsorption of a1D antibody with the immunization peptide showed no staining. (Third row) Double immunostaining with a1B subunit and RBPMS antibodies shows colocalization in RGC somata (filled arrow). Processes in the IPL, putative displaced amacrine cells in the GCL (open arrows) and RGC axons throughout the NFL (arrowheads) showed immunostaining. Control preadsorption of a1B antibody with the immunization peptide showed no staining. (Bottom row) Double immunostaining with a1A subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Labelling in the IPL and in putative displaced amacrine cells in the GCL (open arrows) was also seen. Control preadsorption of a1A antibody with the immunization peptide showed no staining. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g001
Rna Binding Protein Multiple Splice Rbpms Polypeptide, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna binding protein multiple splice rbpms polypeptide/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
rna binding protein multiple splice rbpms polypeptide - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ptbp1  (Boster Bio)
92
Boster Bio ptbp1
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Ptbp1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptbp1/product/Boster Bio
Average 92 stars, based on 1 article reviews
ptbp1 - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
poly(a) tract kit  (Promega)
90
Promega poly(a) tract kit
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Poly(A) Tract Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly(a) tract kit/product/Promega
Average 90 stars, based on 1 article reviews
poly(a) tract kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
malignant digestive tract tumors  (Anhui Medical University)
90
Anhui Medical University malignant digestive tract tumors
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Malignant Digestive Tract Tumors, supplied by Anhui Medical University, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/malignant digestive tract tumors/product/Anhui Medical University
Average 90 stars, based on 1 article reviews
malignant digestive tract tumors - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
poly(a) tract isolation system  (Promega)
90
Promega poly(a) tract isolation system
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Poly(A) Tract Isolation System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly(a) tract isolation system/product/Promega
Average 90 stars, based on 1 article reviews
poly(a) tract isolation system - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
poly tract system 1000 mrna extraction kit  (Promega)
90
Promega poly tract system 1000 mrna extraction kit
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Poly Tract System 1000 Mrna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly tract system 1000 mrna extraction kit/product/Promega
Average 90 stars, based on 1 article reviews
poly tract system 1000 mrna extraction kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
data on census tract boundaries  (Esri inc)
90
Esri inc data on census tract boundaries
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Data On Census Tract Boundaries, supplied by Esri inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/data on census tract boundaries/product/Esri inc
Average 90 stars, based on 1 article reviews
data on census tract boundaries - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
gastrointestinal samples  (Blackwell Science Ltd)
90
Blackwell Science Ltd gastrointestinal samples
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Gastrointestinal Samples, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gastrointestinal samples/product/Blackwell Science Ltd
Average 90 stars, based on 1 article reviews
gastrointestinal samples - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
poly(a) tract system  (Promega)
90
Promega poly(a) tract system
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Poly(A) Tract System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly(a) tract system/product/Promega
Average 90 stars, based on 1 article reviews
poly(a) tract system - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
poly(a) tract system 1000  (Promega)
90
Promega poly(a) tract system 1000
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Poly(A) Tract System 1000, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly(a) tract system 1000/product/Promega
Average 90 stars, based on 1 article reviews
poly(a) tract system 1000 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
genital tract  (Maxion Technologies Inc)
90
Maxion Technologies Inc genital tract
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Genital Tract, supplied by Maxion Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genital tract/product/Maxion Technologies Inc
Average 90 stars, based on 1 article reviews
genital tract - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
biliary tract cancer cell line  (Hasegawa Co Ltd)
90
Hasegawa Co Ltd biliary tract cancer cell line
Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of <t>PTBP1</t> and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.
Biliary Tract Cancer Cell Line, supplied by Hasegawa Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biliary tract cancer cell line/product/Hasegawa Co Ltd
Average 90 stars, based on 1 article reviews
biliary tract cancer cell line - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Expression of L-, P/Q-, N-type Ca channels in the proximal retinal. Ca channel a1 subunits co-express with the ganglion cell antibody RBPMS in vertical sections showing the inner plexiform layer (IPL), the ganglion cell layer (GCL) and the nerve fiber layer (NFL). (Top row) Double immunostaining with a1C subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). RGC axons (arrowhead) as well as putative Mu¨ller and neuronal cell processes in the IPL showed immunostaining. Control preadsorption of a1C antibody with the immunization peptide showed no staining. (Second row) Double immunostaining with a1D subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Numerous processes in the IPL as well as the RGC axons throughout the NFL (arrowhead) were also labelled. Control preadsorption of a1D antibody with the immunization peptide showed no staining. (Third row) Double immunostaining with a1B subunit and RBPMS antibodies shows colocalization in RGC somata (filled arrow). Processes in the IPL, putative displaced amacrine cells in the GCL (open arrows) and RGC axons throughout the NFL (arrowheads) showed immunostaining. Control preadsorption of a1B antibody with the immunization peptide showed no staining. (Bottom row) Double immunostaining with a1A subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Labelling in the IPL and in putative displaced amacrine cells in the GCL (open arrows) was also seen. Control preadsorption of a1A antibody with the immunization peptide showed no staining. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g001

Journal: PloS one

Article Title: Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.

doi: 10.1371/journal.pone.0084507

Figure Lengend Snippet: Figure 1. Expression of L-, P/Q-, N-type Ca channels in the proximal retinal. Ca channel a1 subunits co-express with the ganglion cell antibody RBPMS in vertical sections showing the inner plexiform layer (IPL), the ganglion cell layer (GCL) and the nerve fiber layer (NFL). (Top row) Double immunostaining with a1C subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). RGC axons (arrowhead) as well as putative Mu¨ller and neuronal cell processes in the IPL showed immunostaining. Control preadsorption of a1C antibody with the immunization peptide showed no staining. (Second row) Double immunostaining with a1D subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Numerous processes in the IPL as well as the RGC axons throughout the NFL (arrowhead) were also labelled. Control preadsorption of a1D antibody with the immunization peptide showed no staining. (Third row) Double immunostaining with a1B subunit and RBPMS antibodies shows colocalization in RGC somata (filled arrow). Processes in the IPL, putative displaced amacrine cells in the GCL (open arrows) and RGC axons throughout the NFL (arrowheads) showed immunostaining. Control preadsorption of a1B antibody with the immunization peptide showed no staining. (Bottom row) Double immunostaining with a1A subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Labelling in the IPL and in putative displaced amacrine cells in the GCL (open arrows) was also seen. Control preadsorption of a1A antibody with the immunization peptide showed no staining. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g001

Article Snippet: A guinea pig polyclonal antibody was generated against the N-terminus of the RNA Binding Protein Multiple Splice (RBPMS) polypeptide (RBPMS4-24), GGKAEKENTPSEANLQEEEVR, by a commercial vendor (ProSci, Poway, CA).

Techniques: Expressing, Double Immunostaining, Immunostaining, Control, Staining

Figure 2. Localization of L-type Ca channels in rat retina. (Top row) Rat wholemount retina triple labelled with RBPMS (red), a1C VGCC subunit (green), and NF-M (white) antibodies. a1C colocalized with RBPMS, which labels RGC somata (filled arrow), and NF-M, which labels RGC axons (arrowhead), as well as putative Mu¨ller cell endfeet. Stack of five z-axis optical sections each of 0.3 mm thickness. (Lower row) Rat wholemount retina labelled with RBPMS (red), a1D VGCC subunit (green), and NF-M (white) antibodies. a1D was localized to RGC somata (RBPMS) (filled arrow), RGC axons (NF-M) (arrowhead) and putative displaced amacrine cells (open arrow). Stack of four optical sections each of 0.3 mm thickness. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g002

Journal: PloS one

Article Title: Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.

doi: 10.1371/journal.pone.0084507

Figure Lengend Snippet: Figure 2. Localization of L-type Ca channels in rat retina. (Top row) Rat wholemount retina triple labelled with RBPMS (red), a1C VGCC subunit (green), and NF-M (white) antibodies. a1C colocalized with RBPMS, which labels RGC somata (filled arrow), and NF-M, which labels RGC axons (arrowhead), as well as putative Mu¨ller cell endfeet. Stack of five z-axis optical sections each of 0.3 mm thickness. (Lower row) Rat wholemount retina labelled with RBPMS (red), a1D VGCC subunit (green), and NF-M (white) antibodies. a1D was localized to RGC somata (RBPMS) (filled arrow), RGC axons (NF-M) (arrowhead) and putative displaced amacrine cells (open arrow). Stack of four optical sections each of 0.3 mm thickness. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g002

Article Snippet: A guinea pig polyclonal antibody was generated against the N-terminus of the RNA Binding Protein Multiple Splice (RBPMS) polypeptide (RBPMS4-24), GGKAEKENTPSEANLQEEEVR, by a commercial vendor (ProSci, Poway, CA).

Techniques:

Figure 3. Localization of N-type Ca channels in ganglion cells in rat retina. Rat wholemount retina labelled with RBPMS (red), a1B VGCC subunit (green), and NF-M (white) antibodies. a1B colocalized with RBPMS in RGC somata (filled arrow), NF-M in RGC axons (arrowhead) and putative displaced amacrine cells (open arrow). Stack of six optical sections each of 0.3 mm thickness. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g003

Journal: PloS one

Article Title: Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.

doi: 10.1371/journal.pone.0084507

Figure Lengend Snippet: Figure 3. Localization of N-type Ca channels in ganglion cells in rat retina. Rat wholemount retina labelled with RBPMS (red), a1B VGCC subunit (green), and NF-M (white) antibodies. a1B colocalized with RBPMS in RGC somata (filled arrow), NF-M in RGC axons (arrowhead) and putative displaced amacrine cells (open arrow). Stack of six optical sections each of 0.3 mm thickness. Scale bar is 20 mm. doi:10.1371/journal.pone.0084507.g003

Article Snippet: A guinea pig polyclonal antibody was generated against the N-terminus of the RNA Binding Protein Multiple Splice (RBPMS) polypeptide (RBPMS4-24), GGKAEKENTPSEANLQEEEVR, by a commercial vendor (ProSci, Poway, CA).

Techniques:

Figure 4. P/Q-type Ca channel expression in ganglion cells in rat retina. Retina labelled with RBPMS (red) and a1A VGCC subunit (green) antibodies. a1A was colocalized with RBPMS in RGC somata (filled arrow). Additional cell bodies in the GCL staining for a1A and not RBPMS are likely to be displaced amacrine cells (open arrow). Scale bar is 20 mm. Stack of 10 optical sections each of 0.3 mm thickness. doi:10.1371/journal.pone.0084507.g004

Journal: PloS one

Article Title: Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.

doi: 10.1371/journal.pone.0084507

Figure Lengend Snippet: Figure 4. P/Q-type Ca channel expression in ganglion cells in rat retina. Retina labelled with RBPMS (red) and a1A VGCC subunit (green) antibodies. a1A was colocalized with RBPMS in RGC somata (filled arrow). Additional cell bodies in the GCL staining for a1A and not RBPMS are likely to be displaced amacrine cells (open arrow). Scale bar is 20 mm. Stack of 10 optical sections each of 0.3 mm thickness. doi:10.1371/journal.pone.0084507.g004

Article Snippet: A guinea pig polyclonal antibody was generated against the N-terminus of the RNA Binding Protein Multiple Splice (RBPMS) polypeptide (RBPMS4-24), GGKAEKENTPSEANLQEEEVR, by a commercial vendor (ProSci, Poway, CA).

Techniques: Expressing, Staining

Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of PTBP1 and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Cardioprotective efficacy of Xin-shu-bao tablet in heart failure with reduced ejection fraction by modulating THBD/ARRB1/FGF1/STIM1 signaling.

doi: 10.1016/j.biopha.2023.115119

Figure Lengend Snippet: Fig. 7. Effect of XSB on the expression of the target proteins in the MI-induced pro- and post- HFrEF mice. Western blot analysis of the proteins selected from differentially expressed proteins. The intensities of the bands from the Western blots were quantified and normalized to the corresponding intensity of the GAPDH band. (A) Expression levels of the THBD and FGF1 proteins after 8 weeks of XSB administration; expression levels of PTBP1 and STIM1 proteins after 6 weeks of XSB administration; expression levels of ARRB1 and HIP1 proteins after 4 weeks of XSB administration. (B) Expression levels of THBD, ARRB1, and FGF1 proteins after 8, 6, and 4 weeks of XSB administration. (C) Schematic representation of the differentially expressed proteins expressed after 4, 6, and 8 weeks of XSB administration to the MI-induced pro- and post- HFrEF mice. All data are expressed as the mean ± SD, n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: The membranes were blocked with 5 % skim milk powder and incubated with the primary antibodies against huntingtin interacting protein 1 (HIP1, BOSTER, BM5424, 1:1000), arrestin β 1 (ARRB1, ABclonal, A10742, 1:1000), fibroblast growth factor 1 (FGF1, ABclonal, A5900, 1:1000), thrombomodulin (THBD, ABclonal, A4155, 1:500), PTBP1 (BOSTER, A01798, 1:1000), Stromal Interaction Molecule 1 (STIM1, BOSTER, PB9406, 1:1000), GAPDH (Proteintech, 60004–1-Ig, 1:20000) for overnight at 4 °C.

Techniques: Expressing, Western Blot