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Image Search Results
Journal: Proteomics
Article Title: Proteome-wide changes induced by the Hsp90 inhibitor, geldanamycin in anaplastic large cell lymphoma cells.
doi: 10.1002/pmic.200700108
Figure Lengend Snippet: Figure 6. Immunoblot validation of differentially expressed pro- teins as identified by LC-MS/MS. Total cell lysate was extracted from control and GA-treated cells after 12 h and proteins sepa- rated by SDS-PAGE. Immunoblots were probed with anti-USP9 (1:1000), anti-GAP1 (1:300), anti-PCLN (1:300), anti-STCN (1:300), anti-tankyrase (1:1000), anti-SOCS-4 (1:1000), anti-NEMO (1:1000), anti-OPG (1:500), and anti-actin (1:500). Immunoblots were then incubated at room temperature for 1 h followed by probing with respective HRP-conjugated secondary antibodies and visualized by chemiluminescence. The left panels depict the overexpressed proteins as identified by LC-MS/MS in control and cells exposed to 10 mM GA. The right panels depict the under- expressed proteins as identified in the same samples. Both cICAT- and densitometry fold-change is listed below the 10 mM GA lanes. Actin served as a loading control.
Article Snippet: The following antibodies were used for immunoblot analysis: mouse polyclonal antibodies against PARP, p21, and p27; goat polyclonal antibodies against ras GTPase-activating protein 3 (RARS3), paracellin-1 (PCLN1), and stanniocalcin-1 (STCN1); and rabbit polyclonal antibody against actin from Santa Cruz Biotechnology (Santa Cruz, CA); mouse polyclonal antibodies against Hsp90 and NEMO (Becton Dickinson Pharmingen, San Diego); rabbit polyclonal antibodies against phospho-ALK and ALK (Cell Signaling, Beverly, MA); rabbit polyclonal antibody against ubiquitin-specific protease 9 (USP9) (Abgent, San Diego); mouse polyclonal antibodies against tankyrase (TNKS) and
Techniques: Western Blot, Biomarker Discovery, Liquid Chromatography with Mass Spectroscopy, Control, SDS Page, Incubation