Journal: Pain

Article Title: Neuroanatomical evidence and a mouse calcitonin gene–related peptide model in line with human functional magnetic resonance imaging data support the involvement of peptidergic Edinger–Westphal nucleus in migraine

doi: 10.1097/j.pain.0000000000003294

Figure Lengend Snippet: Antibodies used for immunostainings.

Article Snippet: TPH2 , Rabbit anti-TPH2 (Cat. No.: 348003, Synaptic Systems GmbH) 1:500 , Alexa 647-conjugated donkey antirabbit (Cat. No.: 711-605-152, Jackson) 1:500 or Alexa 488-conjugated donkey antirabbit (Cat. No.: 711-545-152, Jackson) 1:500.

Techniques:

Journal: Pain

Article Title: Neuroanatomical evidence and a mouse calcitonin gene–related peptide model in line with human functional magnetic resonance imaging data support the involvement of peptidergic Edinger–Westphal nucleus in migraine

doi: 10.1097/j.pain.0000000000003294

Figure Lengend Snippet: Summary of statistical results by Student t test analyses.

Article Snippet: TPH2 , Rabbit anti-TPH2 (Cat. No.: 348003, Synaptic Systems GmbH) 1:500 , Alexa 647-conjugated donkey antirabbit (Cat. No.: 711-605-152, Jackson) 1:500 or Alexa 488-conjugated donkey antirabbit (Cat. No.: 711-545-152, Jackson) 1:500.

Techniques:

Journal: Pain

Article Title: Neuroanatomical evidence and a mouse calcitonin gene–related peptide model in line with human functional magnetic resonance imaging data support the involvement of peptidergic Edinger–Westphal nucleus in migraine

doi: 10.1097/j.pain.0000000000003294

Figure Lengend Snippet: Calcitonin gene–related peptide receptor component ( Crcp ) mRNA expression. (A) Representative fluorescence images showing the Crcp mRNA transcripts (red) coexpressed with urocortin 1 peptide (UCN1, green) in the mouse centrally projecting Edinger–Westphal (EWcp) nucleus. (B) In the mouse dorsal raphe nucleus (DRN), the tryptophan hydroxylase-2 (TPH2, green) immunoreactive cells also contained Crcp mRNA transcripts (red). (C) Neurons (neuronal marker NeuN, green) of the mouse spinal trigeminal nucleus express also Crcp mRNA (red). (D) Representative fluorescence images showing the CRCP mRNA transcripts (red) coexpressed with UCN1 (green) in the human EWcp. (E) In the human DRN, the TPH2 (green) immunoreactive cells also contained CRCP mRNA transcripts (red). Nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole (DAPI, blue).

Article Snippet: TPH2 , Rabbit anti-TPH2 (Cat. No.: 348003, Synaptic Systems GmbH) 1:500 , Alexa 647-conjugated donkey antirabbit (Cat. No.: 711-605-152, Jackson) 1:500 or Alexa 488-conjugated donkey antirabbit (Cat. No.: 711-545-152, Jackson) 1:500.

Techniques: Expressing, Fluorescence, Marker

Journal: Pain

Article Title: Neuroanatomical evidence and a mouse calcitonin gene–related peptide model in line with human functional magnetic resonance imaging data support the involvement of peptidergic Edinger–Westphal nucleus in migraine

doi: 10.1097/j.pain.0000000000003294

Figure Lengend Snippet: Serotonin (5-HT) and tryptophan hydroxylase-2 (TPH2) immunoreactivity in the dorsal raphe nucleus (DRN). (A) Specific signal density (SSD) of 5-HT in the DRN (n = 6; * P = 0.03; Student t test). (A′ and A″) Representative fluorescence images showing the 5-HT (green) immunoreactivity in the DRN, 4 hours after saline (control) and calcitonin gene–related peptide (CGRP) injection. (B) SSD of the TPH2 in the DRN (n = 6; * P = 0.01; Student t test). (B′ and B″) Representative fluorescence images illustrate TPH2 (red) immunosignal in the DRN. a.u., arbitrary unit.

Article Snippet: TPH2 , Rabbit anti-TPH2 (Cat. No.: 348003, Synaptic Systems GmbH) 1:500 , Alexa 647-conjugated donkey antirabbit (Cat. No.: 711-605-152, Jackson) 1:500 or Alexa 488-conjugated donkey antirabbit (Cat. No.: 711-545-152, Jackson) 1:500.

Techniques: Fluorescence, Saline, Control, Injection

Journal: Foods

Article Title: Fermented Dendrobium officinale Ameliorates Sleep Deprivation-Induced Depressive-like Behaviors by Attenuating Neuroinflammation and Restoring 5-HT Synthesis via the Gut–Brain Axis

doi: 10.3390/foods15122237

Figure Lengend Snippet: Effects of FDO on hippocampal 5-HT signaling, synaptic markers, and stress-related indicators in SD mice. ( A ) The experimental design. ( B , C ) Quantification of protein levels of PSD-95, SYN, TPH 2 , 5-HT 1A and 5-HT 2A in hippocampal tissue. ( D ) Hippocampal 5-HT levels. ( E ) Hippocampal 5-HIAA levels. ( F ) 5-HIAA/5-HT ratio. ( G ) Hippocampal CORT levels. ( H ) Hippocampal BDNF levels. The expressions of 5-HT 1A ( I ) and 5-HT 2A ( J ) in hippocampus tissue were stained with immunohistochemistry. Scale bar = 100 µm and 50 µm. Data are expressed as mean ± SD ( n = 3–6). The grey dots indicate the values of each independent experiment. * p < 0.05, ** p < 0.01, and *** p < 0.001. ns: no significant difference compared with the SD group. NC: normal control; SD: sleep deprivation; DO: unfermented Dendrobium officinale ; FDO: fermented Dendrobium officinale .

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with primary antibodies against PSD-95 (Proteintech, 1:10,000, rabbit), SYN (Proteintech, 1:40,000, rabbit), TPH 2 (ABclonal, Woburn, MA, USA, 1:1000, rabbit), 5-HT 1A (HUABIO, 1:2000, rabbit), 5-HT 2A (HUABIO, 1:2000, rabbit), and β-actin (ZENBIO, Durham, NC, USA, 1:10,000, rabbit), which were diluted in blocking solution.

Techniques: Staining, Immunohistochemistry, Control