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Image Search Results
Journal: The Journal of Veterinary Medical Science
Article Title: The cytotoxicity and molecular mechanisms of the Clostridium perfringens NetB toxin
doi: 10.1292/jvms.20-0623
Figure Lengend Snippet: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of purified native necrotic enteritis toxin B-like (NetB) (approximately 33 kDa) stained with Coomassie Brilliant Blue in each step of purification of the toxin. Lane 1: ammonium sulphate precipitation of culture supernatant, 2: after anion chromatography, 3: after cation chromatography. CNEOP004 strain was inoculated into trypticase peptone glucose medium and cultured at 37°C for 36 hr, after which NetB was purified.
Article Snippet: NetB was purified from the supernatants of C. perfringens CNEOP004 cultured in 3 l of
Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Purification, Staining, Chromatography, Cell Culture