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  • 99
    Thermo Fisher four well chambered coverslides
    Four Well Chambered Coverslides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/four well chambered coverslides/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
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    four well chambered coverslides - by Bioz Stars, 2020-11
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    94
    Thermo Fisher optimem
    Optimem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 21004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optimem/product/Thermo Fisher
    Average 94 stars, based on 21004 article reviews
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    optimem - by Bioz Stars, 2020-11
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    93
    Horizon Discovery dharmafect 1
    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using <t>DharmaFECT</t> 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).
    Dharmafect 1, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 93/100, based on 2196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dharmafect 1/product/Horizon Discovery
    Average 93 stars, based on 2196 article reviews
    Price from $9.99 to $1999.99
    dharmafect 1 - by Bioz Stars, 2020-11
    93/100 stars
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    99
    Thermo Fisher hela cells
    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using <t>DharmaFECT</t> 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).
    Hela Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 48229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hela cells/product/Thermo Fisher
    Average 99 stars, based on 48229 article reviews
    Price from $9.99 to $1999.99
    hela cells - by Bioz Stars, 2020-11
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    89
    Horizon Discovery dhamafect 1
    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using <t>DharmaFECT</t> 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).
    Dhamafect 1, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 89/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dhamafect 1/product/Horizon Discovery
    Average 89 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    dhamafect 1 - by Bioz Stars, 2020-11
    89/100 stars
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    99
    Thermo Fisher dharmafect 1
    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using <t>DharmaFECT</t> 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).
    Dharmafect 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dharmafect 1/product/Thermo Fisher
    Average 99 stars, based on 1486 article reviews
    Price from $9.99 to $1999.99
    dharmafect 1 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher hypure molecular biology grade water
    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using <t>DharmaFECT</t> 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).
    Hypure Molecular Biology Grade Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hypure molecular biology grade water/product/Thermo Fisher
    Average 97 stars, based on 22 article reviews
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    Image Search Results


    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using DharmaFECT 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Peptide-substituted oligonucleotide synthesis and non-toxic, passive cell delivery

    doi: 10.1038/sigtrans.2016.19

    Figure Lengend Snippet: ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using DharmaFECT 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).

    Article Snippet: TP ODN and DharmaFECT 1 (Dharmacon) were mixed and allowed to form a complex at room temperature (20 min).

    Techniques: Stable Transfection, Transfection, Plasmid Preparation, Luciferase, Expressing, Activity Assay, IA, Negative Control

    Localization of fluorescein-labeled ODNs in HeLa cells. ( a ) Lipid transfection: 100 nM fluorescein-labeled 4K (left) and 6K (right) were transfected into HeLa cells using DharmaFECT 1 (Dharmacon) reagent for 18 h followed by washing. ( b ) Passive transfection: HeLa cells were incubated with 1 μM fluorescein-labeled 4K (left) and 6K (right) for 90 h followed by washing. Cells were stained with Hoechst 33258 to visualize the nuclei.

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Peptide-substituted oligonucleotide synthesis and non-toxic, passive cell delivery

    doi: 10.1038/sigtrans.2016.19

    Figure Lengend Snippet: Localization of fluorescein-labeled ODNs in HeLa cells. ( a ) Lipid transfection: 100 nM fluorescein-labeled 4K (left) and 6K (right) were transfected into HeLa cells using DharmaFECT 1 (Dharmacon) reagent for 18 h followed by washing. ( b ) Passive transfection: HeLa cells were incubated with 1 μM fluorescein-labeled 4K (left) and 6K (right) for 90 h followed by washing. Cells were stained with Hoechst 33258 to visualize the nuclei.

    Article Snippet: TP ODN and DharmaFECT 1 (Dharmacon) were mixed and allowed to form a complex at room temperature (20 min).

    Techniques: Labeling, Transfection, Incubation, Staining

    ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using DharmaFECT 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Peptide-substituted oligonucleotide synthesis and non-toxic, passive cell delivery

    doi: 10.1038/sigtrans.2016.19

    Figure Lengend Snippet: ( a ) A stably transfected vector in HeLa cells expresses two luciferase genes. Firefly luciferase is expressed constitutively, whereas expression of the renilla luciferase gene is under the control of miR-15b as part of the RNA-induced silencing complex (RISC). In the presence of an anti-miR-15b oligonucleotide (TP ODN in this figure), the RISC dissociates from the renilla gene. This leads to an increase in renilla luciferase activity. Comparison of the activity of renilla luciferase to the unregulated firefly luciferase yields an indication of the activity of an oligonucleotide as an anti-miR-15b oligonucleotide. ( a ) Adapted from the Integrated DNA Technologies Inc. (Coralville, IA, USA) website. The stably transfected HeLa cell line and assay protocols were developed by miRagen Therapeutics, Inc. ( b ) Lipid transfection using DharmaFECT 1 (Dharmacon). ( c ) Passive transfection using TP ODNs. Positive and negative controls were purchased from Dharmacon: miRIDIAN Hairpin Inhibitor (IH-300587-07) and miRIDIAN Hairpin Inhibitor Negative Control #1 (IN-001005-01).

    Article Snippet: In an eppendorf tube, 0.25 μl DharmaFECT 1 (purchased from Dharmacon/ThermoFisher, now GE Dharmacon, Lafayette, CO, USA) was added to 50 μl of the reduced serum medium and this solution was added to the TP ODN in the same medium.

    Techniques: Stable Transfection, Transfection, Plasmid Preparation, Luciferase, Expressing, Activity Assay, IA, Negative Control

    Localization of fluorescein-labeled ODNs in HeLa cells. ( a ) Lipid transfection: 100 nM fluorescein-labeled 4K (left) and 6K (right) were transfected into HeLa cells using DharmaFECT 1 (Dharmacon) reagent for 18 h followed by washing. ( b ) Passive transfection: HeLa cells were incubated with 1 μM fluorescein-labeled 4K (left) and 6K (right) for 90 h followed by washing. Cells were stained with Hoechst 33258 to visualize the nuclei.

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Peptide-substituted oligonucleotide synthesis and non-toxic, passive cell delivery

    doi: 10.1038/sigtrans.2016.19

    Figure Lengend Snippet: Localization of fluorescein-labeled ODNs in HeLa cells. ( a ) Lipid transfection: 100 nM fluorescein-labeled 4K (left) and 6K (right) were transfected into HeLa cells using DharmaFECT 1 (Dharmacon) reagent for 18 h followed by washing. ( b ) Passive transfection: HeLa cells were incubated with 1 μM fluorescein-labeled 4K (left) and 6K (right) for 90 h followed by washing. Cells were stained with Hoechst 33258 to visualize the nuclei.

    Article Snippet: In an eppendorf tube, 0.25 μl DharmaFECT 1 (purchased from Dharmacon/ThermoFisher, now GE Dharmacon, Lafayette, CO, USA) was added to 50 μl of the reduced serum medium and this solution was added to the TP ODN in the same medium.

    Techniques: Labeling, Transfection, Incubation, Staining