toxicity assay Search Results


toxic substances  (Disease Registry)
86
Disease Registry toxic substances
Toxic Substances, supplied by Disease Registry, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silicone elastomer  (World Precision Instruments)
96
World Precision Instruments silicone elastomer
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cell counting kit  (Rockland Immunochemicals)
92
Rockland Immunochemicals cell counting kit
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biofixlumi 10 luminometer  (MACHEREY NAGEL)
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concentrated hcl  (Chem Impex International)
95
Chem Impex International concentrated hcl
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death domain fadd  (Boster Bio)
91
Boster Bio death domain fadd
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fadd  (Boster Bio)
90
Boster Bio fadd
Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis <t>of</t> <t>caspase-9,</t> -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of <t>FADD</t> or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Fadd, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acetone  (Chem Impex International)
95
Chem Impex International acetone
Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis <t>of</t> <t>caspase-9,</t> -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of <t>FADD</t> or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Acetone, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fas  (Boster Bio)
90
Boster Bio fas
Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis <t>of</t> <t>caspase-9,</t> -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of <t>FADD</t> or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Fas, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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toxics campaigner greenpeace international  (Greenpeace Research Laboratories)
86
Greenpeace Research Laboratories toxics campaigner greenpeace international
Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis <t>of</t> <t>caspase-9,</t> -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of <t>FADD</t> or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Toxics Campaigner Greenpeace International, supplied by Greenpeace Research Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acute systemic toxicity testing  (Toxikon Inc)
90
Toxikon Inc acute systemic toxicity testing
Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis <t>of</t> <t>caspase-9,</t> -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of <t>FADD</t> or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Acute Systemic Toxicity Testing, supplied by Toxikon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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toxic pollutants  (Monsanto Technology LLC)
90
Monsanto Technology LLC toxic pollutants
Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis <t>of</t> <t>caspase-9,</t> -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of <t>FADD</t> or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Toxic Pollutants, supplied by Monsanto Technology LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis of caspase-9, -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of FADD or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Scutellarein selectively targets multiple myeloma cells by increasing mitochondrial superoxide production and activating intrinsic apoptosis pathway.

doi: 10.1016/j.biopha.2018.09.024

Figure Lengend Snippet: Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis of caspase-9, -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of FADD or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Antibodies for detecting FADD (PA1464, BosterBio, Pleasanton, USA), Apaf-1 (PA1249-1, BosterBio), active Caspase-3 (269518, Novus Biologicals, Littleton, USA) Bax (AF820, R& D Systems, Minneapolis, USA), Bcl-2 (RP1003, BosterBio) and GAPDH (HPA040067, Atlas Antibodies, Bromma, Sweden) were applied following manufacturer’s instructions.

Techniques: In Vitro, Activity Assay, Knockdown, shRNA, Negative Control, Transfection