total-smad3 Search Results


sept9  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc sept9
Figure 4. Dissociation of SEPT2 from Myosin II Alters Myosin II Activity and Increases the Number of Binucleated Cells (A) Representative western blots of CHO-K1 whole-cell lysates prepared after incubating cells with PBS, the TAT-CCT3-pt, or TAT-CCT5 for 1.5 hr. Each lysate was probed with antibodies against the indicated proteins. (B) Quantification of the western blots showing relative levels of myosin II, MYPT1, pMYPT1, SEPT2, <t>SEPT9,</t> MLC, pMLC, and ppMLC. Each exper- iment was done a minimum of three times, normalized to PBS transduction. The error bars reflect the standard deviation. *p < 0.05. (C) Representative western blots of CHO-K1 whole-cell lysates prepared after transfecting with nonspecific shRNAi (shRNAi Scramble) or shRNAi silencing SEPT2. Each lysate was probed with antibodies against the indicated proteins. (D) A representative recording of cell division shown over time depicting cells that were transduced with the TAT-CCT5 or TAT-CCT3-pt. Recordings were started at the beginning of karyokinesis. The number on each panel indicates the time elapsed from the starting point, expressed in minutes.
Sept9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total-smad3/PathScan+Total+Smad3+Sandwich+ELISA+Kit/pm17981136-265-5-27
Average 93 stars, based on 1 article reviews
sept9 - by Bioz Stars, 2026-09
93/100 stars
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total smad3  (US Biological Life Sciences)
90
US Biological Life Sciences total smad3
Figure 4. Dissociation of SEPT2 from Myosin II Alters Myosin II Activity and Increases the Number of Binucleated Cells (A) Representative western blots of CHO-K1 whole-cell lysates prepared after incubating cells with PBS, the TAT-CCT3-pt, or TAT-CCT5 for 1.5 hr. Each lysate was probed with antibodies against the indicated proteins. (B) Quantification of the western blots showing relative levels of myosin II, MYPT1, pMYPT1, SEPT2, <t>SEPT9,</t> MLC, pMLC, and ppMLC. Each exper- iment was done a minimum of three times, normalized to PBS transduction. The error bars reflect the standard deviation. *p < 0.05. (C) Representative western blots of CHO-K1 whole-cell lysates prepared after transfecting with nonspecific shRNAi (shRNAi Scramble) or shRNAi silencing SEPT2. Each lysate was probed with antibodies against the indicated proteins. (D) A representative recording of cell division shown over time depicting cells that were transduced with the TAT-CCT5 or TAT-CCT3-pt. Recordings were started at the beginning of karyokinesis. The number on each panel indicates the time elapsed from the starting point, expressed in minutes.
Total Smad3, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total-smad3/total+smad3+antibody/pmc04831072-44-63-66
Average 90 stars, based on 1 article reviews
total smad3 - by Bioz Stars, 2026-09
90/100 stars
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total smad3  (AnaSpec)
90
AnaSpec total smad3
Figure 4. Dissociation of SEPT2 from Myosin II Alters Myosin II Activity and Increases the Number of Binucleated Cells (A) Representative western blots of CHO-K1 whole-cell lysates prepared after incubating cells with PBS, the TAT-CCT3-pt, or TAT-CCT5 for 1.5 hr. Each lysate was probed with antibodies against the indicated proteins. (B) Quantification of the western blots showing relative levels of myosin II, MYPT1, pMYPT1, SEPT2, <t>SEPT9,</t> MLC, pMLC, and ppMLC. Each exper- iment was done a minimum of three times, normalized to PBS transduction. The error bars reflect the standard deviation. *p < 0.05. (C) Representative western blots of CHO-K1 whole-cell lysates prepared after transfecting with nonspecific shRNAi (shRNAi Scramble) or shRNAi silencing SEPT2. Each lysate was probed with antibodies against the indicated proteins. (D) A representative recording of cell division shown over time depicting cells that were transduced with the TAT-CCT5 or TAT-CCT3-pt. Recordings were started at the beginning of karyokinesis. The number on each panel indicates the time elapsed from the starting point, expressed in minutes.
Total Smad3, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total-smad3/total+smad3+antibody/pm17804483-83-35-37
Average 90 stars, based on 1 article reviews
total smad3 - by Bioz Stars, 2026-09
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alphalisa surefire ultra total smad3 assay kit -500 assay points  (PerkinElmer)
N/A
The AlphaLISA® SureFire® Ultra™ total SMAD3 assay is a sandwich immunoassay for quantitative detection of SMAD3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
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Image Search Results


Figure 4. Dissociation of SEPT2 from Myosin II Alters Myosin II Activity and Increases the Number of Binucleated Cells (A) Representative western blots of CHO-K1 whole-cell lysates prepared after incubating cells with PBS, the TAT-CCT3-pt, or TAT-CCT5 for 1.5 hr. Each lysate was probed with antibodies against the indicated proteins. (B) Quantification of the western blots showing relative levels of myosin II, MYPT1, pMYPT1, SEPT2, SEPT9, MLC, pMLC, and ppMLC. Each exper- iment was done a minimum of three times, normalized to PBS transduction. The error bars reflect the standard deviation. *p < 0.05. (C) Representative western blots of CHO-K1 whole-cell lysates prepared after transfecting with nonspecific shRNAi (shRNAi Scramble) or shRNAi silencing SEPT2. Each lysate was probed with antibodies against the indicated proteins. (D) A representative recording of cell division shown over time depicting cells that were transduced with the TAT-CCT5 or TAT-CCT3-pt. Recordings were started at the beginning of karyokinesis. The number on each panel indicates the time elapsed from the starting point, expressed in minutes.

Journal: Developmental cell

Article Title: Mammalian SEPT2 is required for scaffolding nonmuscle myosin II and its kinases.

doi: 10.1016/j.devcel.2007.09.001

Figure Lengend Snippet: Figure 4. Dissociation of SEPT2 from Myosin II Alters Myosin II Activity and Increases the Number of Binucleated Cells (A) Representative western blots of CHO-K1 whole-cell lysates prepared after incubating cells with PBS, the TAT-CCT3-pt, or TAT-CCT5 for 1.5 hr. Each lysate was probed with antibodies against the indicated proteins. (B) Quantification of the western blots showing relative levels of myosin II, MYPT1, pMYPT1, SEPT2, SEPT9, MLC, pMLC, and ppMLC. Each exper- iment was done a minimum of three times, normalized to PBS transduction. The error bars reflect the standard deviation. *p < 0.05. (C) Representative western blots of CHO-K1 whole-cell lysates prepared after transfecting with nonspecific shRNAi (shRNAi Scramble) or shRNAi silencing SEPT2. Each lysate was probed with antibodies against the indicated proteins. (D) A representative recording of cell division shown over time depicting cells that were transduced with the TAT-CCT5 or TAT-CCT3-pt. Recordings were started at the beginning of karyokinesis. The number on each panel indicates the time elapsed from the starting point, expressed in minutes.

Article Snippet: Collected samples were probed for SEPT9 (1:1000; Surka et al., 2002), SEPT2 (1:1000), myosin II (1:1000), MYPT1 or pMYPT1 (1:1000; Upstate Biotechnology), and pMLC or ppMLC (1:1000; Cell Signaling Technology).

Techniques: Activity Assay, Western Blot, Transduction, Standard Deviation