total ige Search Results


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  • 95
    Thermo Fisher total ige
    Impact of probiotic treatment on mast cell degranulation and BLG-specific antibody responses after an oral challenge with β-lactoglobulin (BLG). Mice received by gavage a 6-week probiotic treatment or PBS and 5 exposures (1 per week) to CT (diamonds, nonsensitized mice; n = 10) or whey protein (WP) with CT (circles, sensitized mice; n = 20 for each group). After BLG challenge, the levels of mouse mast cell protease-1 (mMCP-1) and BLG-specific <t>IgE,</t> <t>IgG1,</t> and IgG2a in plasma were determined by ELISA. Each point represents a mouse, and the central horizontal line represents the median of each group. Data were compared to WP-sensitized mice treated with PBS using the Mann-Whitney U test (**, P
    Total Ige, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Acris Antibodies total ige
    Levels of total immunoglobulin (Ig) E, ovalbumin (OVA)-specific <t>IgE,</t> IgG 1 , and IgG 2a as determined by enzyme-linked immunosorbent assay in the sera of each group ( * P
    Total Ige, supplied by Acris Antibodies, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche total ige
    Levels of total immunoglobulin (Ig) E, ovalbumin (OVA)-specific <t>IgE,</t> IgG 1 , and IgG 2a as determined by enzyme-linked immunosorbent assay in the sera of each group ( * P
    Total Ige, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson total ige
    The level of total <t>IgE</t> in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of <t>rebamipide</t> treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.
    Total Ige, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    EUROIMMUN total immunoglobulin e
    The level of total <t>IgE</t> in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of <t>rebamipide</t> treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.
    Total Immunoglobulin E, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    International Stem Cell Corporation total immunoglobulin e
    The level of total <t>IgE</t> in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of <t>rebamipide</t> treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.
    Total Immunoglobulin E, supplied by International Stem Cell Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher total ige levels
    The level of total <t>IgE</t> in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of <t>rebamipide</t> treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.
    Total Ige Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson murine total ige kit
    The level of total <t>IgE</t> in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of <t>rebamipide</t> treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.
    Murine Total Ige Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher immunecap total ige assay
    The level of total <t>IgE</t> in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of <t>rebamipide</t> treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.
    Immunecap Total Ige Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher ige antibodies
    A , <t>IgE</t> sensitization to inhalant and food allergen extracts in 6-10 year olds (n = 616) as measured by <t>ImmunoCAP.</t> B , Comparison of IgE results from microchip assay with results from the extract assay. C , Comparison of food-specific components with ImmunoCAP singleplex and ISAC. Class sensitization (on x-axis ) was determined by extract testing and statistics reflect the χ 2 test with Yates correction.
    Ige Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pharmingen total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Siemens Healthineers total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Kamiya total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Kamiya, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Shin Jin Medics total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Shin Jin Medics, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bethyl total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Diagnostic Products Corp total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Diagnostic Products Corp, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific total ige
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Ige, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    College of American Pathologists total serum ige data
    Skin inflammation induces polyclonal ‘natural <t>IgE’</t> with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for <t>phosphorylcholine</t> (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p
    Total Serum Ige Data, supplied by College of American Pathologists, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson total ige elisa kit
    Effects of C 60 derivatives on B cell responses. C 60 -P, C 60 (OH) 36 , or N -acetylcysteine (NAC) was added to purified B cells 30 min before the addition of IL-4 and anti-CD40. After incubation of the cells for 10 days, the amount of total <t>IgE</t> in the supernatants was measured by <t>ELISA.</t> Data are means ± SDs for three to six independent cultures ( n = 3 to 6). * P
    Total Ige Elisa Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bethyl iga total ige
    Concentrations of (A) faecal <t>IgA</t> (µg IgA/µg TP), (B) serum IgA (g/l) and (c) serum <t>IgE</t> (g/l) in puppies at 7 weeks, 12–13 months and 15–18 months of age and their mothers at 7 weeks pp. Mean and sds are indicated in blue. pp, postpartum; TP, total protein
    Iga Total Ige, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher ova specific ige total ige
    Concentrations of (A) faecal <t>IgA</t> (µg IgA/µg TP), (B) serum IgA (g/l) and (c) serum <t>IgE</t> (g/l) in puppies at 7 weeks, 12–13 months and 15–18 months of age and their mothers at 7 weeks pp. Mean and sds are indicated in blue. pp, postpartum; TP, total protein
    Ova Specific Ige Total Ige, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of probiotic treatment on mast cell degranulation and BLG-specific antibody responses after an oral challenge with β-lactoglobulin (BLG). Mice received by gavage a 6-week probiotic treatment or PBS and 5 exposures (1 per week) to CT (diamonds, nonsensitized mice; n = 10) or whey protein (WP) with CT (circles, sensitized mice; n = 20 for each group). After BLG challenge, the levels of mouse mast cell protease-1 (mMCP-1) and BLG-specific IgE, IgG1, and IgG2a in plasma were determined by ELISA. Each point represents a mouse, and the central horizontal line represents the median of each group. Data were compared to WP-sensitized mice treated with PBS using the Mann-Whitney U test (**, P

    Journal: Applied and Environmental Microbiology

    Article Title: Three Novel Candidate Probiotic Strains with Prophylactic Properties in a Murine Model of Cow's Milk Allergy

    doi: 10.1128/AEM.03440-15

    Figure Lengend Snippet: Impact of probiotic treatment on mast cell degranulation and BLG-specific antibody responses after an oral challenge with β-lactoglobulin (BLG). Mice received by gavage a 6-week probiotic treatment or PBS and 5 exposures (1 per week) to CT (diamonds, nonsensitized mice; n = 10) or whey protein (WP) with CT (circles, sensitized mice; n = 20 for each group). After BLG challenge, the levels of mouse mast cell protease-1 (mMCP-1) and BLG-specific IgE, IgG1, and IgG2a in plasma were determined by ELISA. Each point represents a mouse, and the central horizontal line represents the median of each group. Data were compared to WP-sensitized mice treated with PBS using the Mann-Whitney U test (**, P

    Article Snippet: Total IgE, IgG1, and IgG2a were quantified using mouse ELISA Ready-SET-Go! kits (eBioscience, San Diego, CA) according to the manufacturer's instructions.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Levels of total immunoglobulin (Ig) E, ovalbumin (OVA)-specific IgE, IgG 1 , and IgG 2a as determined by enzyme-linked immunosorbent assay in the sera of each group ( * P

    Journal: Korean Journal of Pediatrics

    Article Title: Hu.4-1BB-Fc fusion protein inhibits allergic inflammation and airway hyperresponsiveness in a murine model of asthma

    doi: 10.3345/kjp.2011.54.9.373

    Figure Lengend Snippet: Levels of total immunoglobulin (Ig) E, ovalbumin (OVA)-specific IgE, IgG 1 , and IgG 2a as determined by enzyme-linked immunosorbent assay in the sera of each group ( * P

    Article Snippet: Total IgE and OVA-specific IgE levels, however, were not significantly decreased in Hu.4-1BB-Fc treated mice though they were slightly decreased.

    Techniques: Enzyme-linked Immunosorbent Assay

    The level of total IgE in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of rebamipide treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.

    Journal: Clinical and Translational Allergy

    Article Title: Downregulation of IL-8, ECP, and total IgE in the tears of patients with atopic keratoconjunctivitis treated with rebamipide eyedrops

    doi: 10.1186/2045-7022-4-40

    Figure Lengend Snippet: The level of total IgE in the tears of each eye and the statistical analysis of the change. a . The level of total IgE in the tears of each eye at before- and 2 and 4–6 weeks after the start of rebamipide treatment. b . The statistical analysis of the change in the tear level of total IgE in the six eyes. Pretreatment levels are recorded as 1 on the y axis. The length of treatment is indicated on the x axis. Data are the mean (95% confidence limits) ± SD.

    Article Snippet: In patient 4, whose left eye was treated with rebamipide and right eye with tacrolimus, an immunosuppressant, rebamipide reduced the tear level of IL-8, ECP, and total IgE and ameliorated her subjective symptoms in the left eye although amelioration was weaker than in the right eye treated with the immunosuppressant tacrolimus eyedrops (Additional file : Figure S3a).

    Techniques:

    A , IgE sensitization to inhalant and food allergen extracts in 6-10 year olds (n = 616) as measured by ImmunoCAP. B , Comparison of IgE results from microchip assay with results from the extract assay. C , Comparison of food-specific components with ImmunoCAP singleplex and ISAC. Class sensitization (on x-axis ) was determined by extract testing and statistics reflect the χ 2 test with Yates correction.

    Journal: The Journal of allergy and clinical immunology

    Article Title: Allergen sensitization in a birth cohort at mid-childhood: focus on food component IgE and IgG4 responses

    doi: 10.1016/j.jaci.2017.07.029

    Figure Lengend Snippet: A , IgE sensitization to inhalant and food allergen extracts in 6-10 year olds (n = 616) as measured by ImmunoCAP. B , Comparison of IgE results from microchip assay with results from the extract assay. C , Comparison of food-specific components with ImmunoCAP singleplex and ISAC. Class sensitization (on x-axis ) was determined by extract testing and statistics reflect the χ 2 test with Yates correction.

    Article Snippet: Allergen extract–specific IgE antibodies were measured by ImmunoCAP (Thermo Fisher Scientific/Phadia, Kalamazoo, MI) to 8 inhalants and 5 foods where values > 0.35 kU/L were considered positive.

    Techniques: MicroChIP Assay

    A , Representative results of samples that were tested for IgE and IgG4 to cow's milk and peanut components by ImmunoCAP, and for IgE to components by ISAC. B , Specific IgG4 levels from samples that were positive to the component-relevant food specific IgE by extract testing where threshold of detection of specific IgG4 was 0.07 μg/ml. Data represents geometric means where error bars are the 95% CI. Values less than the threshold of detection are shown below the dotted line and are not included in the geometric mean. C , Milk (n=20) and peanut (n=23)-specific components were plotted for IgE and IgG4. Where IgE and IgG4 were less than the detection limit they were depicted with the values 0.25 kU/L and 0.04 μg/mL, respectively.

    Journal: The Journal of allergy and clinical immunology

    Article Title: Allergen sensitization in a birth cohort at mid-childhood: focus on food component IgE and IgG4 responses

    doi: 10.1016/j.jaci.2017.07.029

    Figure Lengend Snippet: A , Representative results of samples that were tested for IgE and IgG4 to cow's milk and peanut components by ImmunoCAP, and for IgE to components by ISAC. B , Specific IgG4 levels from samples that were positive to the component-relevant food specific IgE by extract testing where threshold of detection of specific IgG4 was 0.07 μg/ml. Data represents geometric means where error bars are the 95% CI. Values less than the threshold of detection are shown below the dotted line and are not included in the geometric mean. C , Milk (n=20) and peanut (n=23)-specific components were plotted for IgE and IgG4. Where IgE and IgG4 were less than the detection limit they were depicted with the values 0.25 kU/L and 0.04 μg/mL, respectively.

    Article Snippet: Allergen extract–specific IgE antibodies were measured by ImmunoCAP (Thermo Fisher Scientific/Phadia, Kalamazoo, MI) to 8 inhalants and 5 foods where values > 0.35 kU/L were considered positive.

    Techniques:

    Skin inflammation induces polyclonal ‘natural IgE’ with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for phosphorylcholine (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p

    Journal: bioRxiv

    Article Title: Natural IgE promotes epithelial hyperplasia and inflammation-driven tumour growth

    doi: 10.1101/782805

    Figure Lengend Snippet: Skin inflammation induces polyclonal ‘natural IgE’ with similar characteristics to that in naïve animals (a-d) High-throughput sequencing and heavy-chain repertoire analysis of IgE in sorted FSC hi CD95 hi CD138 + plasma cells from skin draining LNs of WT mice 7 days after topical exposures to TPA (TPA 2x a week for 2 weeks) (n=6) and in whole spleen from UT naïve mice (n=5). (a) % of the total repertoire consisting of clone sizes ≤ 10. (b) Igh-V gene family usage expressed as mean of total repertoire ± SEM. V genes not used in the repertoire of any mouse removed for clarity. (b) Paired DJ repertoire shown as average Igh-D and Igh-J use of total repertoire in UT (left) and TPA-treated (right) mice. (d) Physicochemical properties of the CDRH3 regions; length (number of a.a.), % mutation compared to germline, aliphatic index and isoelectric point (pI) presented as mean ± SEM. (e) Level of IgE in serum specific for phosphorylcholine (PC) in UT WT (n=6), UT Tcrb −/− (n=5), 10x TPA-treated (2x/week) WT mice (n=6) and in WT mice undergone DMBA-TPA carcinogenesis (single low-dose DMBA + 20 weeks TPA, 2x/week) (n=14). Data presented as means ± SEM. Statistics by two-tailed Student’s t-test for unpaired data (a, b and d) and one-way ANOVA multiple comparison (e); *p

    Article Snippet: For detection of anti-phosphorylcholine (anti-PC) specific IgE; total IgE was captured with anti-mouse IgE (PharMingen), followed by addition of PC-BSA at 50 mg/ml (Sigma).

    Techniques: Next-Generation Sequencing, Mouse Assay, Mutagenesis, Two Tailed Test

    Effects of C 60 derivatives on B cell responses. C 60 -P, C 60 (OH) 36 , or N -acetylcysteine (NAC) was added to purified B cells 30 min before the addition of IL-4 and anti-CD40. After incubation of the cells for 10 days, the amount of total IgE in the supernatants was measured by ELISA. Data are means ± SDs for three to six independent cultures ( n = 3 to 6). * P

    Journal: Nanoscale Research Letters

    Article Title: Potential Suppressive Effects of Two C60 Fullerene Derivatives on Acquired Immunity

    doi: 10.1186/s11671-016-1663-7

    Figure Lengend Snippet: Effects of C 60 derivatives on B cell responses. C 60 -P, C 60 (OH) 36 , or N -acetylcysteine (NAC) was added to purified B cells 30 min before the addition of IL-4 and anti-CD40. After incubation of the cells for 10 days, the amount of total IgE in the supernatants was measured by ELISA. Data are means ± SDs for three to six independent cultures ( n = 3 to 6). * P

    Article Snippet: After incubation of the cells for 10 days at 37 °C (95 % room air, 5 % CO2 ), the amount of total IgE released into an aliquot of culture supernatant was measured with a total IgE ELISA kit (BD Biosciences) in accordance with the manufacturer’s instructions.

    Techniques: Purification, Incubation, Enzyme-linked Immunosorbent Assay

    Effects of C 60 -P on acquired immune response to ovalbumin (OVA) in vivo. C57BL/6 mice were treated weekly with OVA alone (10 μg/mouse) or with a mixture of C 60 -P at varying dose rates and OVA by intraperitoneal injection. Seven days after the last treatment, spleens and plasma were collected. a Levels of OVA-specific IgG1 and IgE in the plasma were evaluated by ELISA. b Splenocytes were re-stimulated with OVA (100 μg mL). After incubation of the cells for 72 h, levels of IL-4 in the supernatants were measured by ELISA. Data are means ± SEMs ( n = 4 or 5). * P

    Journal: Nanoscale Research Letters

    Article Title: Potential Suppressive Effects of Two C60 Fullerene Derivatives on Acquired Immunity

    doi: 10.1186/s11671-016-1663-7

    Figure Lengend Snippet: Effects of C 60 -P on acquired immune response to ovalbumin (OVA) in vivo. C57BL/6 mice were treated weekly with OVA alone (10 μg/mouse) or with a mixture of C 60 -P at varying dose rates and OVA by intraperitoneal injection. Seven days after the last treatment, spleens and plasma were collected. a Levels of OVA-specific IgG1 and IgE in the plasma were evaluated by ELISA. b Splenocytes were re-stimulated with OVA (100 μg mL). After incubation of the cells for 72 h, levels of IL-4 in the supernatants were measured by ELISA. Data are means ± SEMs ( n = 4 or 5). * P

    Article Snippet: After incubation of the cells for 10 days at 37 °C (95 % room air, 5 % CO2 ), the amount of total IgE released into an aliquot of culture supernatant was measured with a total IgE ELISA kit (BD Biosciences) in accordance with the manufacturer’s instructions.

    Techniques: In Vivo, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Incubation

    Concentrations of (A) faecal IgA (µg IgA/µg TP), (B) serum IgA (g/l) and (c) serum IgE (g/l) in puppies at 7 weeks, 12–13 months and 15–18 months of age and their mothers at 7 weeks pp. Mean and sds are indicated in blue. pp, postpartum; TP, total protein

    Journal: Veterinary Record Open

    Article Title: Immunoglobulins in dogs: correspondence and maturation in 15 litters of German shepherd dogs and their dams

    doi: 10.1136/vetreco-2016-000173

    Figure Lengend Snippet: Concentrations of (A) faecal IgA (µg IgA/µg TP), (B) serum IgA (g/l) and (c) serum IgE (g/l) in puppies at 7 weeks, 12–13 months and 15–18 months of age and their mothers at 7 weeks pp. Mean and sds are indicated in blue. pp, postpartum; TP, total protein

    Article Snippet: Serum were assayed for IgA total IgE (Bethyl Laboratories) and IgG against CDV (VMRD) within 45 months by ELISA (Bethyl Laboratories) as follows: a 96-well plate was coated overnight at 4°C with a 1:100 dilution of goat anti-canine IgA, IgE (Bethyl Laboratories) or CDV (VMRD) Affinity purified in 50 μl of borate buffer (6.2 g H3 BO3 /l, 9.54 g Na2 B4 O7 10 H2 O/l and 4.4 g NaCl/l, pH 7) and then washed with PBS-Tween-20.

    Techniques: