Journal: Biomedicines

Article Title: Obesity-Related Genes Expression in Testes and Sperm Parameters Respond to GLP-1 and Caloric Restriction

doi: 10.3390/biomedicines10102609

Figure Lengend Snippet: Fasting blood glucose and hormones levels. Fasting blood glucose and hormone measurements (insulin, ghrelin, active GLP-1, and leptin) were performed in rats from the CTR, GLP-1, and CR groups. Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) value was also calculated. Results are expressed as mean ± SEM (n = 5 for control and GLP-1 condition and n = 6 to CR condition). * indicates statistically significant differences as compared to the control group (* p < 0.05, ** p < 0.01 and *** p < 0.001). ↑ indicates statistically increase in comparison to the control, ↓ indicates statistically decrease in comparison to the control, and ↔ indicates no statistically significant difference in comparison to the control.

Article Snippet: Plasma total and active GLP-1 levels (EZGLP1T-36K and EGLP-35K; Merck Millipore, Billerica, MA, USA), insulin (EZRMI-13K; Merck Millipore), total ghrelin (EZRGRT-91K; Merck Millipore, Billerica, MA, USA), and leptin (EZRL-83K; Merck Millipore, Billerica, MA, USA) were determined with an enzyme-linked immunosorbent assay (ELISA) using specific commercial kits according to the manufacturer’s instructions.

Techniques:

Journal: Gut and Liver

Article Title: Serum Concentrations of Insulin, Ghrelin, Adiponectin, Leptin, Leptin Receptor and Lipocalin-2 in Children with Celiac Disease Who Do and Do Not Adhere to a Gluten-Free Diet

doi: 10.5009/gnl15404

Figure Lengend Snippet: A gluten-free diet (GFD) significantly decreased the total serum ghrelin concentration in prepubertal girls and boys (p<0.05) but did not have a significant effect in older children (p>0.05). The data consist of the median, Q1–Q3 (quartiles 1–3), and range.

Article Snippet: Total Ghrelin ELISA kit (sensitivity, 100 pg/mL; inter- and intra-assay CV, 1.9% and 7.8%) and Leptin (Sandwich) ELISA (sensitivity, 1.0 ng/mL; intra- and interassay CV, 6.9% and 11.5%) were from DRG International Inc., Springfield, NJ, USA.

Techniques: Concentration Assay

Journal: International Journal of Peptides

Article Title: The Avian Proghrelin System

doi: 10.1155/2010/749401

Figure Lengend Snippet: Expression of preproghrelin mRNA (a) in proventriculus and plasma total (acylated + des-acyl) ghrelin levels (b) in two groups of neonatal broiler chicks from hatch to 8 days posthatch. Feed was provided immediately after hatching (Fed) or was withheld for the first 48 hours after hatch (delayed feeding, DF). Values represent the mean ± SEM ( n = 6).

Article Snippet: The commercial assay used to measure total ghrelin (Linco Research, St. Charles, MO) detects both acylated and des-acyl ghrelin, whereas, Kayia et al. [ ] and Shousha et al. [ ] utilized a specific N-terminal directed radioimmunoassay (RIA) and sample purification (HPLC) to measure only the acylated form of ghrelin.

Techniques: Expressing