Journal:

Article Title: Green and black tea extracts inhibit HMG-CoA reductase and activate AMP-kinase to decrease cholesterol synthesis in hepatoma cells

doi: 10.1016/j.jnutbio.2008.07.011

Figure Lengend Snippet: Phosphorylation of AMP-kinase by tea extract. Phosphorylated AMP-kinase was measured by immunoquantitation with an antibody specific for the phosphorylated enzyme. A, Enzyme phosphorylation in hepatoma cells after a 3-h treatment with green (grey bars) or black (black bars) tea extract; each data set included an untreated sample and samples treated with the AMP-kinase activators AICAR or metformin (1 mM each). Values represent the mean and standard deviation of 2 experiments; values that are statistically different from untreated controls are indicated with asterisks. The immunoblots below the graph illustrate the increase in AMP-kinase phosphorylation with each treatment (GTE, green tea extract; BTE, black tea extract). B, Enzyme phosphorylation in hepatoma cells at various times after treatment with 15 μg/ml of green (circles) or black (boxes) tea extract. Values represent the mean and standard deviation of 4 experiments; closed symbols are statistically different from untreated controls. Statistical significance was determined by one-way analysis of variance with Dunnett's post-hoc text, p < 0.05.

Article Snippet: The membrane was blocked with 0.05% Tween-20 and 5% defatted milk, and then incubated in this same buffer with rabbit antibody to total AMP-kinase (Anti-AMPK α-pan, 1:2000; Upstate USA, Inc., Charlottesville, VA) or to phosphorylated AMP-kinase (Anti-phospho-AMPKα, 1:500; Upstate USA, Inc.) overnight at 4°C.

Techniques: Standard Deviation, Western Blot