Journal: iScience

Article Title: OsFKBP12 transduces the sucrose signal from OsNIN8 to the OsTOR pathway in a loosely binding manner for cell division

doi: 10.1016/j.isci.2024.111555

Figure Lengend Snippet:

Article Snippet: Torin2 , Immunoway , Cat# MC0120.

Techniques: Binding Assay, Virus, Recombinant, Magnetic Beads, SYBR Green Assay, Luciferase, Lysis, Mutagenesis, CRISPR, Knock-Out, Over Expression, Knockdown, Plasmid Preparation, Software, Real-time Polymerase Chain Reaction

Journal: Plant Physiology

Article Title: TARGET OF RAPAMYCIN is essential for asexual vegetative reproduction in Kalanchoë

doi: 10.1093/plphys/kiab589

Figure Lengend Snippet: Torin2, AZD-8055, and mock treatment of K. daigremontiana . A, B, Area and leaf number of plantlets planted on media containing mock solution or 100-µM Torin2. C–F, 100-µM Torin2 brushed directly onto the leaf margins reduced plantlet formation ( n = 25). G–J, 2-µM, 20-µM, and 40-µM AZD-8055 brushed directly onto the leaf margins reduced plantlet formation independent of leaf size (>3 cm, n = 32, <3 cm, n = 45). Error bars show SEM. Two-way ANOVA (repeated measures) with Sidak’s multiple comparisons tests (95% confidence limits: ns; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001) (A, B, E, and F) and least squares mean with Tukey’s P -value adjustment (95% confidence limits) (I and J).

Article Snippet: Torin2 solutions (100-μM Torin2, 0.5% [v/v] DMSO, and 0.5% [v/v] Tween-20 [AppliChem, USA]) or AZD-8055 solutions (2 μM, 20 μM, or 40 μM AZD-8055 [Selleckchem, USA], 0.5% [v/v] DMSO and 0.5% [v/v] Tween-20 [AppliChem, USA]) and mock solutions (0.5% [v/v] DMSO and 0.5% [v/v] Tween-20) were applied by brushing the margins of plastochron 2 (P 2 ) leaves.

Techniques: